FastQCFastQC Report
Thu 26 May 2016
SRR1545936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545936_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1319762
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136281.0326104252130308No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44490.33710623582130717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG650.044.0000041
GCGTAAG650.044.0000041
CTTCGAA302.5280042E-644.027
TGTCACG254.443201E-544.01
TAATACG302.5280042E-644.01
CTCACGT302.5280042E-644.044
AGTCACG207.856906E-444.01
TAGCGTC254.443201E-544.019
GGCGTAA207.856906E-444.08
TAGAACG254.443201E-544.01
ATTACCG254.443201E-544.01
ATTACAA750.044.012
GTGATAT302.5280042E-644.030
TCTAGCG600.044.01
ACGGGCC502.7284841E-1144.05
AGTATTA254.443201E-544.06
TAACCGA207.856906E-444.043
TACGCAC207.856906E-444.034
TACGCAA302.5280042E-644.01
TACGATG551.8189894E-1244.01
Produced by FastQC (version 0.11.5)