FastQCFastQC Report
Thu 26 May 2016
SRR1545935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545935_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2004103
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194210.9690619693698378No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33550.16740656543101826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC207.857884E-444.00000441
ACTACGT207.857884E-444.00000439
GATATCG408.3182385E-944.00000411
TCGTATC207.857884E-444.00000437
CTATACG502.7284841E-1144.01
TACGCAC254.444029E-544.017
CGTACTT351.446806E-744.019
CGTTTTT150350.043.4146961
CGGTCTA2350.042.1276631
CTCACGA2400.041.2524
TGCGACG650.040.6153831
ATCGCAC557.8216544E-1140.010
ACGGGAT10600.039.6415065
GGTACGC501.3496901E-939.6000028
AACGGGA7700.038.8571434
TACGGGA6800.038.1764684
CATACGA4800.038.04166418
AGGGCGC4750.037.9789476
GTTTTTT178600.037.7917142
CACGGTA357.292916E-637.71428735
Produced by FastQC (version 0.11.5)