FastQCFastQC Report
Thu 26 May 2016
SRR1545934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545934_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1384123
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136900.9890739479078087No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50450.3644907280639076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC207.8570406E-444.041
AGATCGC207.8570406E-444.019
CGTATAT302.5280933E-644.014
CGACGAA207.8570406E-444.034
CGCATTA207.8570406E-444.032
CGCATGA207.8570406E-444.036
CCGCTAT254.4433138E-544.011
CCGTAAG254.4433138E-544.037
TCTAACG502.7284841E-1144.01
GGTATGA207.8570406E-444.02
GCGATAT1000.044.09
TCGTACA254.4433138E-544.034
CTATTCG302.5280933E-644.040
CGTAAAT454.802132E-1044.016
TACTAAG454.802132E-1044.01
CGGTCAT254.4433138E-544.023
ACTTTCG254.4433138E-544.027
CCACGTT254.4433138E-544.023
CGTCAAA254.4433138E-544.038
TCGGTTA207.8570406E-444.024
Produced by FastQC (version 0.11.5)