FastQCFastQC Report
Thu 26 May 2016
SRR1545933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545933_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1376762
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134630.977874171425417No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20410.14824639262269007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT254.443302E-544.016
TATGCGA254.443302E-544.013
TACGACG207.857025E-444.01
TACTACG207.857025E-444.01
GCGTAAG408.314601E-944.01
TAATCGC254.443302E-544.039
TTATCGG351.4463694E-743.9999962
CGTTTTT95950.043.0371
GCCGATT1250.042.249
CACGACG2100.041.9047626
TATAGCG750.041.0666661
TAATGCG750.041.0666661
CGGTCTA2150.040.93023331
ACGGGTA700.040.857145
ATGGTCG603.6379788E-1240.33333244
TACGGGA5100.040.117654
TAATACG557.8216544E-1140.01
CACATCG557.8216544E-1140.028
CGTAAAT557.8216544E-1140.016
TCGATCC501.3478711E-939.614
Produced by FastQC (version 0.11.5)