##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545933_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1376762
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5264286783046	31.0	31.0	33.0	30.0	34.0
2	31.921703242826283	31.0	31.0	34.0	30.0	34.0
3	31.962187364264846	33.0	31.0	34.0	30.0	34.0
4	35.732705434926295	37.0	35.0	37.0	35.0	37.0
5	35.66198515066511	37.0	35.0	37.0	33.0	37.0
6	35.718123393876354	37.0	35.0	37.0	33.0	37.0
7	36.01071717551763	37.0	35.0	37.0	35.0	37.0
8	36.00875459956042	37.0	35.0	37.0	35.0	37.0
9	37.823084164147474	39.0	38.0	39.0	35.0	39.0
10	37.31523168129277	39.0	37.0	39.0	34.0	39.0
11	36.94364385420283	39.0	37.0	39.0	33.0	39.0
12	36.21552817407802	38.0	35.0	39.0	32.0	39.0
13	35.91123229723075	38.0	35.0	39.0	32.0	39.0
14	36.879425783105575	39.0	35.0	41.0	31.0	41.0
15	37.07792196472593	39.0	35.0	41.0	32.0	41.0
16	37.26132476056137	39.0	35.0	41.0	33.0	41.0
17	37.20635738057849	39.0	35.0	41.0	32.0	41.0
18	37.10869562059383	38.0	35.0	41.0	32.0	41.0
19	36.953092110328434	38.0	35.0	41.0	32.0	41.0
20	36.74391361760421	38.0	35.0	40.0	32.0	41.0
21	36.608593932720396	38.0	35.0	40.0	32.0	41.0
22	36.51896333571089	38.0	35.0	40.0	31.0	41.0
23	36.45383225277862	38.0	35.0	40.0	31.0	41.0
24	36.49416892680071	38.0	35.0	40.0	32.0	41.0
25	36.44253690906635	38.0	35.0	40.0	31.0	41.0
26	36.22327969540124	37.0	35.0	40.0	31.0	41.0
27	36.207279834858895	37.0	35.0	40.0	31.0	41.0
28	36.18732722140791	37.0	35.0	40.0	31.0	41.0
29	36.14130910062887	37.0	35.0	40.0	31.0	41.0
30	36.009420655131386	37.0	35.0	40.0	30.0	41.0
31	35.786796846513774	37.0	35.0	40.0	30.0	41.0
32	35.52621586011235	37.0	34.0	40.0	29.0	41.0
33	35.25515593835391	37.0	34.0	40.0	27.0	41.0
34	34.9767243721137	37.0	34.0	40.0	26.0	41.0
35	34.73516337609551	37.0	34.0	40.0	24.0	41.0
36	34.536694069127414	37.0	34.0	40.0	23.0	41.0
37	34.42790620310555	36.0	34.0	40.0	23.0	41.0
38	34.32570698494003	36.0	33.0	40.0	23.0	41.0
39	34.23113217825593	36.0	33.0	40.0	23.0	41.0
40	34.06456163084106	36.0	33.0	40.0	22.0	41.0
41	34.03759110143947	36.0	33.0	40.0	22.0	41.0
42	34.00824979190303	36.0	33.0	40.0	22.0	41.0
43	33.91577193443747	35.0	33.0	40.0	22.0	41.0
44	33.800225456542236	35.0	33.0	40.0	21.0	41.0
45	33.69781777823618	35.0	33.0	40.0	21.0	41.0
46	33.66743562068099	35.0	33.0	40.0	21.0	41.0
47	33.61021803332748	35.0	33.0	40.0	21.0	41.0
48	33.49114153354029	35.0	33.0	40.0	20.0	41.0
49	33.43650609182996	35.0	33.0	40.0	20.0	41.0
50	33.297160293500255	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	7.0
14	9.0
15	38.0
16	99.0
17	262.0
18	572.0
19	1210.0
20	2293.0
21	3640.0
22	5513.0
23	8208.0
24	12242.0
25	18617.0
26	27272.0
27	32513.0
28	33181.0
29	33099.0
30	36168.0
31	40925.0
32	50647.0
33	66078.0
34	120363.0
35	199150.0
36	89245.0
37	112739.0
38	173975.0
39	308691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.176540317062795	22.701745109176457	27.617046374028337	16.504668199732418
2	34.32132786930494	23.84987383440275	29.625309240086523	12.203489056205793
3	27.683070857562893	24.53212683092648	35.31837746829154	12.46642484321909
4	24.750537856216255	26.10799833231888	34.82344806146596	14.31801574999891
5	21.99232692360771	31.440655683407883	33.31759592435004	13.249421468634376
6	19.3586836359516	40.280309886530866	30.574928709537307	9.786077767980233
7	86.08495876556732	3.0225994035279884	9.69898936780649	1.193452463098197
8	87.76956365733511	1.9891600726923027	9.149366411914333	1.0919098580582554
9	84.14206667528592	3.674346038022549	10.306429143163452	1.877158143528075
10	48.7419757372734	26.086716513093766	15.509652358214417	9.661655391418416
11	40.969753668390034	20.220560997470876	25.888570428294795	12.921114905844291
12	37.44401719396671	21.00326708610493	28.411591836497518	13.141123883430833
13	22.138539558761792	34.51722229404937	28.831998558937567	14.512239588251274
14	16.485638040561838	36.48706167078986	31.95897330112249	15.068326987525804
15	15.336492436601242	22.158513962471364	47.107197903486586	15.397795697440808
16	18.08569672899165	18.180774890649218	44.67119226126229	19.062336119096837
17	18.08032179853889	18.942562331034704	31.82075042745224	31.156365442974167
18	22.633904770759216	22.45398986898244	35.564026316821646	19.3480790434367
19	26.930435325786156	22.80009180962287	29.645356277991404	20.624116586599573
20	28.324648704714395	22.775105646437073	28.705687693297754	20.194557955550778
21	22.599403527988134	24.936263493617634	32.451505779502924	20.012827198891312
22	23.161737468059112	22.12088944930206	30.568391631959628	24.1489814506792
23	19.582978031061288	26.991230147258566	30.1510355457225	23.274756275957646
24	19.904602247883076	22.872362833953872	39.4725450005157	17.75048991764735
25	18.406812506446286	23.682379380023562	36.8120270605958	21.09878105293435
26	17.817676548306824	30.36799388710612	30.372933012387037	21.44139655220002
27	18.223992236857207	29.484544169580506	32.762452769614505	19.529010823947786
28	16.130819996484504	25.941811293455224	39.03005748270217	18.897311227358106
29	17.31068986506019	23.369834437615218	37.785034740935615	21.534440956388977
30	20.104564187564737	26.76671784956296	34.35118052357634	18.777537439295973
31	27.60186582720906	24.390272247490852	28.75958226621595	19.24827965908414
32	27.712197169881215	24.723300032975924	29.789244618895637	17.77525817824722
33	26.524410174017003	25.452184182887095	28.591579372469607	19.431826270626296
34	20.236322617852615	25.901571949254848	31.51125612124681	22.35084931164573
35	21.319080567302116	25.663477057036726	31.476028536522655	21.5414138391385
36	28.72704214671817	24.538300737527617	27.69817877018686	19.036478345567353
37	21.59959382957984	29.710799687963497	29.430431694076393	19.259174788380275
38	21.782268830778303	30.309087554711706	26.336578144951705	21.572065469558282
39	21.88751577977893	28.95002912631232	28.179380314099312	20.98307477980944
40	23.495418961301954	26.608956377354982	26.691541457419653	23.20408320392341
41	20.030767845132274	24.03269410399183	28.82829421497688	27.108243835899014
42	22.586983080590546	25.00228797715219	26.631908783072166	25.778820159185102
43	22.473601101715474	26.340064586326466	27.766164376994716	23.42016993496334
44	21.150641868383932	28.373023078789217	28.41195500747406	22.06438004535279
45	18.94982575056546	33.02749494829172	25.370688615752034	22.651990685390793
46	23.119391732194817	29.652111258155006	27.168239681223046	20.060257328427138
47	22.166721626541115	26.72393630852682	29.371234824900743	21.738107240031322
48	23.06498871990947	24.678702637057096	31.04196658536479	21.214342057668645
49	22.21792873423293	24.00610998850927	31.55200390481434	22.223957372443458
50	20.557801566283786	29.85802920185188	28.647217166075183	20.93695206578915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	425.0
1	1387.0
2	2349.0
3	13157.5
4	23966.0
5	17268.0
6	10570.0
7	10740.5
8	10911.0
9	11098.0
10	11285.0
11	11063.0
12	10841.0
13	10225.5
14	9610.0
15	8865.0
16	8120.0
17	7353.0
18	6586.0
19	6265.5
20	5945.0
21	5609.0
22	5273.0
23	5631.5
24	5990.0
25	7009.0
26	8028.0
27	9702.5
28	11377.0
29	14062.0
30	16747.0
31	19715.0
32	22683.0
33	27071.0
34	31459.0
35	34666.5
36	37874.0
37	39708.0
38	41542.0
39	51363.5
40	61185.0
41	81706.0
42	102227.0
43	111986.0
44	121745.0
45	124618.5
46	127492.0
47	120531.0
48	113570.0
49	110017.0
50	106464.0
51	100933.5
52	95403.0
53	85377.0
54	75351.0
55	69486.0
56	63621.0
57	59663.0
58	55705.0
59	50358.5
60	45012.0
61	40783.0
62	36554.0
63	32798.0
64	29042.0
65	25045.0
66	21048.0
67	17468.0
68	13888.0
69	12407.0
70	10926.0
71	9101.0
72	7276.0
73	5731.5
74	4187.0
75	3263.0
76	2339.0
77	1708.0
78	1077.0
79	793.5
80	510.0
81	421.0
82	332.0
83	229.0
84	126.0
85	91.0
86	56.0
87	41.5
88	27.0
89	20.5
90	14.0
91	8.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1376762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.95786510666044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.972663899985	16.34140572022858
2	6.901820403122032	2.892948419980567
3	2.671665092570918	1.6797718986082435
4	1.5679836291516585	1.314463575568494
5	1.0934681349778506	1.1458378835648682
6	0.7749054263268781	0.9744218037226745
7	0.6075598432447874	0.8913210067263613
8	0.5082709921937519	0.8521819913620092
9	0.41201372768991384	0.7771435314315788
>10	5.022890983541893	24.999628075653025
>50	1.705542799893578	25.640565388741894
>100	0.7594611155338408	21.221166551002284
>500	0.0010523710607858838	0.12931845947096138
>1k	3.507903535952945E-4	0.15005058316101885
>5k	0.0	0.0
>10k+	3.507903535952945E-4	0.9897751107774605
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13463	0.977874171425417	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2041	0.14824639262269007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035009682138234494	0.0
2	0.0	0.0	0.0	0.10328582572732252	0.0
3	0.0	0.0	0.0	0.14752005066961466	0.0
4	0.0	0.0	0.0	0.21274555805578596	0.0
5	0.0	0.0	0.0	0.3787873285288234	0.0
6	0.0	0.0	0.0	0.5309559676981207	0.0
7	0.0	0.0	0.0	0.6304648152694511	0.0
8	0.0	0.0	0.0	0.8750967850652474	0.0
9	0.0	0.0	0.0	0.9822322231438695	0.0
10	0.0	0.0	0.0	1.177327671739923	0.0
11	0.0	0.0	0.0	1.3636343827037642	0.0
12	0.0	0.0	0.0	1.5210326839352044	0.0
13	0.0	0.0	0.0	1.588291948789987	0.0
14	0.0	0.0	0.0	1.6155297720303146	0.0
15	0.0	0.0	0.0	1.6558417504259995	0.0
16	0.0	0.0	0.0	1.7525904985756435	0.0
17	0.0	0.0	0.0	1.8703305291691665	0.0
18	0.0	0.0	0.0	2.034048005392363	0.0
19	0.0	0.0	0.0	2.105374785184367	0.0
20	0.0	0.0	0.0	2.1905746962801125	0.0
21	0.0	0.0	0.0	2.3035208699833376	0.0
22	0.0	0.0	0.0	2.4159586043194103	0.0
23	0.0	0.0	0.0	2.541543128006148	0.0
24	0.0	0.0	0.0	2.6373476316167936	0.0
25	0.0	0.0	0.0	2.7208043220251574	0.0
26	0.0	0.0	0.0	2.8039704756522914	0.0
27	0.0	0.0	0.0	2.902680347075239	0.0
28	0.0	0.0	0.0	2.990712991787978	0.0
29	0.0	0.0	0.0	3.099301113772751	0.0
30	0.0	0.0	0.0	3.2302605679122465	0.0
31	0.0	0.0	0.0	3.342480399662396	0.0
32	0.0	0.0	0.0	3.4448219808507208	0.0
33	0.0	0.0	0.0	3.550650003413807	0.0
34	0.0	0.0	0.0	3.6677363262495626	0.0
35	0.0	0.0	0.0	3.8045791502089688	0.0
36	0.0	0.0	0.0	3.9186874710371145	0.0
37	0.0	0.0	0.0	4.0418750662787035	0.0
38	0.0	0.0	0.0	4.177773645699111	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	25	4.443302E-5	44.0	16
TATGCGA	25	4.443302E-5	44.0	13
TACGACG	20	7.857025E-4	44.0	1
TACTACG	20	7.857025E-4	44.0	1
GCGTAAG	40	8.314601E-9	44.0	1
TAATCGC	25	4.443302E-5	44.0	39
TTATCGG	35	1.4463694E-7	43.999996	2
CGTTTTT	9595	0.0	43.037	1
GCCGATT	125	0.0	42.24	9
CACGACG	210	0.0	41.90476	26
TATAGCG	75	0.0	41.066666	1
TAATGCG	75	0.0	41.066666	1
CGGTCTA	215	0.0	40.930233	31
ACGGGTA	70	0.0	40.85714	5
ATGGTCG	60	3.6379788E-12	40.333332	44
TACGGGA	510	0.0	40.11765	4
TAATACG	55	7.8216544E-11	40.0	1
CACATCG	55	7.8216544E-11	40.0	28
CGTAAAT	55	7.8216544E-11	40.0	16
TCGATCC	50	1.3478711E-9	39.6	14
>>END_MODULE