FastQCFastQC Report
Thu 26 May 2016
SRR1545932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545932_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1520347
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124160.8166556713697596No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25610.1684483871116265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1550.044.028
CCTAACG351.4464968E-744.01
GTATCGA351.4464968E-744.012
ATACCCG254.4435204E-544.018
ACGACTT254.4435204E-544.017
AATCGAC351.4464968E-744.035
CTTACGA351.4464968E-744.044
GCGATAT1050.043.9999969
CGTTTTT91050.042.9368481
CGTATGG2100.041.904762
ACGGGTG900.041.5555575
ACGGGTA1500.041.0666665
CACGGGT1100.040.04
CGGGTAT1000.039.6000026
CCGTTAT452.3517714E-839.1111118
TTACGGG4250.038.823533
TCTAGCG404.1257044E-738.51
TACGGGA4550.038.19784
TGGGCGA6400.038.156256
GCGATAA750.038.133339
Produced by FastQC (version 0.11.5)