FastQCFastQC Report
Thu 26 May 2016
SRR1545931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545931_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1844291
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225511.2227463019664466No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42890.23255549151408322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC302.5285535E-644.038
ACAACGT207.857721E-444.028
CGGAATA207.857721E-444.020
AACCGTA207.857721E-444.020
GTCGATC207.857721E-444.023
GTTTACG502.7284841E-1144.01
TCTTACG254.4438915E-544.01
ACTTACG302.5285535E-644.01
GTAATCG254.4438915E-544.01
TACGCTA207.857721E-444.01
GTTCGTA254.4438915E-544.030
CATACGT207.857721E-444.038
TTAACGC207.857721E-444.029
TCCGTCG351.446715E-744.04
CGTACCC351.446715E-744.012
ACGTCGG302.5285535E-644.02
CGTCGGA351.446715E-744.038
ATACGTC207.857721E-444.039
TATGTCG207.857721E-444.022
CGATACA302.5285535E-644.020
Produced by FastQC (version 0.11.5)