##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545931_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1844291
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558512187068093	31.0	31.0	33.0	30.0	34.0
2	31.94565499696089	31.0	31.0	34.0	30.0	34.0
3	31.964661216695195	33.0	31.0	34.0	30.0	34.0
4	35.73631059306801	37.0	35.0	37.0	35.0	37.0
5	35.679651421603204	37.0	35.0	37.0	33.0	37.0
6	35.7388926151025	37.0	35.0	37.0	33.0	37.0
7	36.01870258001584	37.0	35.0	37.0	35.0	37.0
8	36.018949829500876	37.0	35.0	37.0	35.0	37.0
9	37.848618791719964	39.0	38.0	39.0	35.0	39.0
10	37.35343392121959	39.0	37.0	39.0	34.0	39.0
11	37.006581390897644	39.0	37.0	39.0	33.0	39.0
12	35.948342750683054	37.0	35.0	39.0	32.0	39.0
13	35.49614458889622	37.0	35.0	39.0	31.0	39.0
14	36.352173816387975	38.0	35.0	41.0	31.0	41.0
15	36.6630390757207	38.0	35.0	41.0	32.0	41.0
16	36.91686886722323	38.0	35.0	41.0	32.0	41.0
17	36.883756955925065	38.0	35.0	41.0	32.0	41.0
18	36.77506152770902	38.0	35.0	40.0	32.0	41.0
19	36.58334069840389	37.0	35.0	40.0	32.0	41.0
20	36.295034243511466	36.0	35.0	40.0	32.0	41.0
21	36.13097933026838	35.0	35.0	40.0	31.0	41.0
22	36.01872697963608	35.0	35.0	40.0	31.0	41.0
23	36.00507349436721	35.0	35.0	40.0	31.0	41.0
24	36.01845370388946	35.0	35.0	40.0	31.0	41.0
25	35.97947070174934	35.0	35.0	40.0	31.0	41.0
26	35.73999222465435	35.0	35.0	40.0	31.0	41.0
27	35.716397249674806	35.0	35.0	40.0	31.0	41.0
28	35.74146596171645	36.0	35.0	40.0	31.0	41.0
29	35.6885198702374	36.0	35.0	40.0	31.0	41.0
30	35.57141850174403	36.0	35.0	40.0	30.0	41.0
31	35.255490050105976	35.0	34.0	40.0	30.0	41.0
32	34.92214623397284	35.0	34.0	40.0	29.0	41.0
33	34.5660684783475	35.0	34.0	40.0	26.0	41.0
34	34.263522947300615	35.0	34.0	40.0	23.0	41.0
35	33.97220232598869	35.0	33.0	40.0	23.0	41.0
36	33.732984111509516	35.0	33.0	40.0	21.0	41.0
37	33.618160040904606	35.0	33.0	40.0	21.0	41.0
38	33.55949955836687	35.0	33.0	40.0	21.0	41.0
39	33.50468770926063	35.0	33.0	40.0	20.0	41.0
40	33.26885670428365	35.0	33.0	40.0	18.0	41.0
41	33.25060416170767	35.0	33.0	40.0	18.0	41.0
42	33.205422571600685	35.0	33.0	40.0	18.0	41.0
43	33.12022451988325	35.0	33.0	40.0	18.0	41.0
44	32.9717848213758	35.0	33.0	39.0	18.0	41.0
45	32.862507055556854	35.0	32.0	39.0	18.0	41.0
46	32.861358104550746	35.0	33.0	39.0	18.0	41.0
47	32.815876128008	35.0	33.0	39.0	18.0	41.0
48	32.7496832115973	35.0	32.0	39.0	18.0	41.0
49	32.73969509150129	35.0	32.0	39.0	18.0	41.0
50	32.5468681460789	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	5.0
14	21.0
15	60.0
16	152.0
17	395.0
18	958.0
19	1884.0
20	3287.0
21	5445.0
22	8277.0
23	12393.0
24	18892.0
25	29266.0
26	42315.0
27	51339.0
28	50765.0
29	48136.0
30	50654.0
31	58316.0
32	71819.0
33	95283.0
34	186407.0
35	335344.0
36	105444.0
37	130156.0
38	197161.0
39	340113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.39689398256566	21.462556613896613	28.172343735343286	17.968205668194447
2	35.33108386908573	23.821566119446445	28.74155976470091	12.105790246766915
3	28.157975070094686	22.954132509457565	36.38471369214511	12.503178728302638
4	24.21890037960387	26.290211251911984	35.603708959161004	13.88717940932315
5	21.507018144099817	31.192745613354944	34.25023491412147	13.050001328423768
6	19.019070200960694	39.36255178819395	31.886237041768357	9.732140969077006
7	83.50770024903879	3.764861402023867	11.469502372456407	1.257935976480935
8	85.73424692741004	2.0214272042752475	11.119937146578279	1.1243887217364288
9	83.20189167544602	3.1087827246351036	11.978207343635033	1.7111182562838512
10	55.090763876199574	18.997435871020354	16.84533514505032	9.066465107729746
11	50.043078885056644	16.4462658007874	22.573173105545706	10.937482208610247
12	44.18115145603379	18.60752993968956	25.808562748503356	11.402755855773302
13	20.93964564160428	40.49198309811196	26.002024626265595	12.566346634018167
14	14.30462980082861	41.91589071355876	31.14589834250669	12.63358114310594
15	12.160391174711583	20.47144404001321	54.27489479697076	13.09326998830445
16	14.815503627139101	14.31194968689865	52.34710791301372	18.525438772948522
17	15.694974383109825	15.0971294660116	32.22062028172344	36.98727586915514
18	21.444717780437035	20.35616938975465	38.226125920475674	19.972986909332636
19	28.985664409792168	21.569914942923866	29.373455707369388	20.070964939914578
20	30.93340476096234	20.986655576587427	27.9811591554695	20.09878050698073
21	21.80279576270773	25.858446416536218	32.3691868582561	19.96957096249995
22	21.41728176301896	21.376398843783328	29.70848960386403	27.49782978933368
23	18.10750038903839	28.06466007804625	28.702791479218842	25.125048053696514
24	18.9807899078833	21.227994931385556	43.84340649062431	15.947808670106834
25	16.36596393953015	21.70172711356288	40.777729761734996	21.154579185171972
26	15.46399131156634	31.4114204320251	32.17756850735594	20.947019749052618
27	16.158241839275906	32.543779696371125	33.4272086129575	17.87076985139547
28	14.071098324505188	25.608594305345523	42.98909445418321	17.33121291596608
29	14.751197072479345	22.170199822045436	42.236067952400134	20.842535153075083
30	18.209707687127466	28.089873018954165	35.809207982905086	17.891211311013283
31	29.43998533853931	23.40172998729593	28.9325816804398	18.22570299372496
32	30.83016725668563	23.621868783180094	29.430062826311033	16.117901133823242
33	28.671722629454898	25.19732515096587	27.776798780669644	18.354153438909588
34	18.981061014774784	25.559469736608808	31.14904318244789	24.310426066168517
35	19.717983767203766	24.942593115728485	33.924798201585325	21.414624915482428
36	32.3278159466158	21.953476972993958	27.797023354774275	17.92168372561597
37	20.503814202856276	31.67775584221796	29.738474026062043	18.07995592886372
38	21.418040862315113	31.409360019649828	25.77570459325562	21.39689452477944
39	21.25228610886243	30.459835242919908	27.40597877449925	20.88189987371841
40	25.180733409207118	24.78925505790572	26.76893180089259	23.26107973199457
41	18.246903552638926	22.97598372491109	28.43716094694384	30.33995177550614
42	22.940143393857042	23.934509250438243	25.474125287169976	27.651222068534736
43	23.198182933170525	24.830409084032834	26.313363780444625	25.658044202352016
44	20.337951006647	28.99970774677098	29.352634698103497	21.30970654847852
45	17.50493821202836	36.5948757544227	23.67061380226873	22.229572231280205
46	22.10350752674063	31.482450437593634	27.0996822085018	19.314359827163933
47	21.544159788232985	26.507584757503018	28.644666161684896	23.3035892925791
48	22.99382255837067	23.433612157734327	31.828165945612703	21.7443993382823
49	21.296584974930745	22.74163892791322	33.70422563467479	22.257550462481245
50	19.05973623468314	32.47990691273774	27.77880497166662	20.6815518809125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	736.0
1	1465.0
2	2194.0
3	19392.0
4	36590.0
5	26403.0
6	16216.0
7	16908.5
8	17601.0
9	18505.5
10	19410.0
11	19134.0
12	18858.0
13	17728.0
14	16598.0
15	15224.5
16	13851.0
17	12634.5
18	11418.0
19	10276.0
20	9134.0
21	8435.0
22	7736.0
23	8411.5
24	9087.0
25	8727.5
26	8368.0
27	9515.0
28	10662.0
29	12206.0
30	13750.0
31	18512.5
32	23275.0
33	26539.5
34	29804.0
35	32384.0
36	34964.0
37	38177.5
38	41391.0
39	56274.0
40	71157.0
41	109465.0
42	147773.0
43	172538.0
44	197303.0
45	189503.0
46	181703.0
47	171719.5
48	161736.0
49	154734.0
50	147732.0
51	134189.0
52	120646.0
53	110726.5
54	100807.0
55	90318.5
56	79830.0
57	75390.0
58	70950.0
59	64604.5
60	58259.0
61	53506.0
62	48753.0
63	43408.0
64	38063.0
65	32929.0
66	27795.0
67	23700.0
68	19605.0
69	15187.5
70	10770.0
71	9669.5
72	8569.0
73	7125.5
74	5682.0
75	4145.5
76	2609.0
77	2130.5
78	1652.0
79	1146.5
80	641.0
81	519.0
82	397.0
83	257.5
84	118.0
85	79.5
86	41.0
87	36.0
88	31.0
89	23.0
90	15.0
91	11.5
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1844291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.95021077763028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80974261724586	14.505519128656156
2	7.2139743141174275	2.589847189656373
3	2.4621264962520857	1.3258706870673982
4	1.2766940139422382	0.9166770659520811
5	0.8531912811136009	0.7657481664812772
6	0.606548790347432	0.6532607180191848
7	0.46938761911888743	0.5897924689715849
8	0.37809546882054906	0.5429514687516634
9	0.29491468231991264	0.4764402638154274
>10	2.99773532832772	12.086626341835661
>50	1.0668455915638306	14.025046835695129
>100	1.562483165253881	49.116238103880264
>500	0.0073427836236691245	0.8586514884647517
>1k	6.118986353057604E-4	0.3088619105293101
>5k	0.0	0.0
>10k+	3.059493176528802E-4	1.238468162223768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22551	1.2227463019664466	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4289	0.23255549151408322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020224574104628825	0.0
2	0.0	0.0	0.0	0.09136302242975756	0.0
3	0.0	0.0	0.0	0.1311072927211595	0.0
4	0.0	0.0	0.0	0.19319077087075737	0.0
5	0.0	0.0	0.0	0.32999130831305906	0.0
6	0.0	0.0	0.0	0.4634843416792686	0.0
7	0.0	0.0	0.0	0.5528953944903489	0.0
8	0.0	0.0	0.0	0.8084407504021871	0.0
9	0.0	0.0	0.0	0.9239322861739281	0.0
10	0.0	0.0	0.0	1.128075775460597	0.0
11	0.0	0.0	0.0	1.2943185213179482	0.0
12	0.0	0.0	0.0	1.4599106106357402	0.0
13	0.0	0.0	0.0	1.5218314246504483	0.0
14	0.0	0.0	0.0	1.5447128462916102	0.0
15	0.0	0.0	0.0	1.5768661236214891	0.0
16	0.0	0.0	0.0	1.6593368400106057	0.0
17	0.0	0.0	0.0	1.756176221648319	0.0
18	0.0	0.0	0.0	1.9281122122268124	0.0
19	0.0	0.0	0.0	1.9942622937486547	0.0
20	0.0	0.0	0.0	2.0676238185839435	0.0
21	0.0	0.0	0.0	2.161806352685124	0.0
22	0.0	0.0	0.0	2.254416466815703	0.0
23	0.0	0.0	0.0	2.362642337895701	0.0
24	0.0	0.0	0.0	2.4368714047837354	0.0
25	0.0	0.0	0.0	2.4992259898248164	0.0
26	0.0	0.0	0.0	2.5675991478568188	0.0
27	0.0	0.0	0.0	2.6518591697297227	0.0
28	0.0	0.0	0.0	2.7192563429523866	0.0
29	0.0	0.0	0.0	2.804709235147816	0.0
30	0.0	0.0	0.0	2.914561747576711	0.0
31	0.0	0.0	0.0	3.010370923026789	0.0
32	5.4221378296592024E-5	0.0	0.0	3.0928416394159055	0.0
33	1.0844275659318405E-4	0.0	0.0	3.176884775775623	0.0
34	1.0844275659318405E-4	0.0	0.0	3.273181943630371	0.0
35	1.0844275659318405E-4	0.0	0.0	3.401144396410328	0.0
36	1.0844275659318405E-4	0.0	0.0	3.5007490683411673	0.0
37	1.0844275659318405E-4	0.0	0.0	3.6066976415327083	0.0
38	1.0844275659318405E-4	0.0	0.0	3.720074543550882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	30	2.5285535E-6	44.0	38
ACAACGT	20	7.857721E-4	44.0	28
CGGAATA	20	7.857721E-4	44.0	20
AACCGTA	20	7.857721E-4	44.0	20
GTCGATC	20	7.857721E-4	44.0	23
GTTTACG	50	2.7284841E-11	44.0	1
TCTTACG	25	4.4438915E-5	44.0	1
ACTTACG	30	2.5285535E-6	44.0	1
GTAATCG	25	4.4438915E-5	44.0	1
TACGCTA	20	7.857721E-4	44.0	1
GTTCGTA	25	4.4438915E-5	44.0	30
CATACGT	20	7.857721E-4	44.0	38
TTAACGC	20	7.857721E-4	44.0	29
TCCGTCG	35	1.446715E-7	44.0	4
CGTACCC	35	1.446715E-7	44.0	12
ACGTCGG	30	2.5285535E-6	44.0	2
CGTCGGA	35	1.446715E-7	44.0	38
ATACGTC	20	7.857721E-4	44.0	39
TATGTCG	20	7.857721E-4	44.0	22
CGATACA	30	2.5285535E-6	44.0	20
>>END_MODULE