FastQCFastQC Report
Thu 26 May 2016
SRR1545930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545930_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1410599
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139580.9895087122562827No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43350.3073162535915593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA207.85709E-444.00000442
ATCTCGC408.314601E-944.00000411
TCGCAGA207.85709E-444.00000412
CGACGGA207.85709E-444.0000043
CGACCCA207.85709E-444.00000420
GTTAGTA207.85709E-444.00000418
ATCACGC207.85709E-444.00000424
ATATCGC207.85709E-444.00000436
AGTGCCG207.85709E-444.0000041
GTTCGGC207.85709E-444.00000432
GTTCGCT207.85709E-444.00000417
CTACGCG207.85709E-444.0000041
CGCTAGC207.85709E-444.00000420
ACGTGAC207.85709E-444.00000431
CGTACGA207.85709E-444.00000440
TGCTCGA207.85709E-444.00000442
CTCTACG207.85709E-444.00000440
TCGGTTA408.314601E-944.00000425
AATACGT207.85709E-444.00000433
CGCCAGT408.314601E-944.00000426
Produced by FastQC (version 0.11.5)