##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545930_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1410599
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59694215010786	31.0	31.0	33.0	30.0	34.0
2	31.987236627843917	31.0	31.0	34.0	30.0	34.0
3	31.974805029636347	33.0	31.0	34.0	30.0	34.0
4	35.755764749585104	37.0	35.0	37.0	35.0	37.0
5	35.69977222442381	37.0	35.0	37.0	33.0	37.0
6	35.77203088900531	37.0	35.0	37.0	33.0	37.0
7	36.06960659974947	37.0	35.0	37.0	35.0	37.0
8	36.0764086746127	37.0	35.0	37.0	35.0	37.0
9	37.91056423547727	39.0	38.0	39.0	35.0	39.0
10	37.43730996548275	39.0	37.0	39.0	35.0	39.0
11	37.01823835122526	39.0	37.0	39.0	33.0	39.0
12	35.96996453279777	37.0	35.0	39.0	32.0	39.0
13	35.50827698020486	37.0	35.0	39.0	31.0	39.0
14	36.426946283103845	38.0	35.0	41.0	31.0	41.0
15	36.740402481499	38.0	35.0	41.0	32.0	41.0
16	36.991471708118326	38.0	35.0	41.0	33.0	41.0
17	36.94361544280125	38.0	35.0	41.0	33.0	41.0
18	36.80715851918227	38.0	35.0	40.0	32.0	41.0
19	36.604441800965404	37.0	35.0	40.0	32.0	41.0
20	36.31852638489039	36.0	35.0	40.0	32.0	41.0
21	36.15310020778407	35.0	35.0	40.0	32.0	41.0
22	36.036254810899486	35.0	35.0	40.0	31.0	41.0
23	36.00613143777927	35.0	35.0	40.0	32.0	41.0
24	36.01021693620937	35.0	35.0	40.0	32.0	41.0
25	35.961312180144745	35.0	35.0	40.0	32.0	41.0
26	35.732345620548436	35.0	35.0	40.0	31.0	41.0
27	35.68939507258973	35.0	35.0	40.0	31.0	41.0
28	35.743087156590924	36.0	35.0	40.0	31.0	41.0
29	35.73767952479762	36.0	35.0	40.0	31.0	41.0
30	35.61723920121877	36.0	35.0	40.0	31.0	41.0
31	35.27163424899635	35.0	35.0	40.0	30.0	41.0
32	34.88406910822991	35.0	34.0	40.0	29.0	41.0
33	34.484409814553956	35.0	34.0	40.0	25.0	41.0
34	34.15918698368566	35.0	34.0	40.0	23.0	41.0
35	33.84750095526794	35.0	34.0	40.0	22.0	41.0
36	33.61129633581195	35.0	33.0	40.0	20.0	41.0
37	33.49910002771872	35.0	33.0	40.0	19.0	41.0
38	33.47812241466214	35.0	33.0	40.0	20.0	41.0
39	33.41191791572233	35.0	33.0	40.0	18.0	41.0
40	33.23950747164857	35.0	33.0	40.0	18.0	41.0
41	33.229296915707444	35.0	33.0	40.0	18.0	41.0
42	33.16642646138272	35.0	33.0	40.0	18.0	41.0
43	33.07528574740235	35.0	33.0	40.0	18.0	41.0
44	32.947255031373196	35.0	33.0	40.0	17.0	41.0
45	32.84677218685112	35.0	33.0	40.0	17.0	41.0
46	32.808602586560745	35.0	33.0	39.0	17.0	41.0
47	32.76800352190807	35.0	33.0	39.0	17.0	41.0
48	32.708663482676506	35.0	33.0	39.0	17.0	41.0
49	32.69941776507711	35.0	33.0	39.0	15.0	41.0
50	32.48205407773577	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	4.0
14	8.0
15	25.0
16	82.0
17	240.0
18	582.0
19	1244.0
20	2342.0
21	3916.0
22	6229.0
23	9551.0
24	14697.0
25	24159.0
26	34920.0
27	40777.0
28	39499.0
29	36165.0
30	37576.0
31	42683.0
32	52719.0
33	70146.0
34	140898.0
35	269908.0
36	74282.0
37	89750.0
38	144297.0
39	273897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.161016702833336	21.088133480882945	28.7493469086537	20.00150290763002
2	34.55617081821269	23.902257126227937	30.00689777888684	11.534674276672535
3	26.08558491818015	23.962444323298115	38.23829451176415	11.713676246757583
4	23.445571703935702	26.25650521516037	37.588286961780064	12.709636119123862
5	20.45237519663632	31.23864400868	36.18484062444394	12.124140170239734
6	18.89431369226832	38.22454148911207	32.58013085221243	10.301013966407178
7	82.9763809558918	3.4797982984533524	12.427273803540198	1.116546942114662
8	85.43406028219216	1.9314489801850137	11.618610249971821	1.0158804876509908
9	82.66998629660166	3.285909035806774	12.646329679802692	1.3977749877888757
10	53.92900462853015	23.15626198515666	16.02056998480787	6.894163401505319
11	48.58545908511207	16.239696752939707	25.989384651484936	9.185459510463286
12	43.06879559676421	19.404522475912714	27.119330156904976	10.4073517704181
13	19.26791384369335	43.82542451823658	26.60649837409498	10.300163263975092
14	12.21027379148858	44.99180844449769	31.51916313566081	11.278754628352921
15	10.342485709971436	20.726372271637793	57.63551512513479	11.295626893255987
16	12.515959532085304	15.851209308953147	55.238235671512605	16.39459548744895
17	13.739694980642975	15.498664042722277	33.606219769048465	37.15542120758628
18	19.29747575320839	20.67199820785354	41.325139178462486	18.705386860475585
19	27.191001836808336	21.29003352476501	31.882909317247492	19.636055321179157
20	30.46443390361116	19.985906696375086	30.599624698443712	18.950034701570043
21	19.76068322747996	26.95117464282904	33.710927060064556	19.57721506962645
22	20.737644078862953	22.169588947674	30.78933134079919	26.303435632663852
23	16.211481788942145	29.13634562338411	30.09111731966349	24.561055268010257
24	17.255151889374655	21.215384386349346	47.057313949605806	14.472149774670193
25	13.638319607485899	23.192842189736417	43.76615891546783	19.40267928730986
26	14.039354912345747	34.2842296074221	33.22857878107102	18.447836699161137
27	14.023404241744112	34.92402872822113	34.78288301636397	16.269684013670787
28	11.775919308038642	26.392475820555667	46.23993069610853	15.59167417529716
29	11.651078726129821	21.555169116098906	46.23043118561689	20.563320972154383
30	15.86907406002698	29.280752361230938	37.42239998752303	17.42777359121905
31	27.836046955938578	24.882053652384553	29.55389873380032	17.728000657876546
32	28.466984593070038	25.294927899424284	30.994279735062907	15.243807772442771
33	26.68284891737482	27.148254039595944	29.0518425151301	17.117054527899143
34	17.966622690077052	26.82271857558385	31.58083906198714	23.62981967235196
35	18.188656024851856	25.15080472905482	35.11444428927002	21.546094956823307
36	31.13684328430688	22.241827762532086	29.72481903078054	16.896509922380492
37	18.65753484867067	31.314852768221158	32.120042620191846	17.907569762916324
38	18.936919705742028	32.22871985589101	26.586861326287625	22.247499112079336
39	19.177597602153412	31.559146149968914	30.20135417648814	19.06190207138953
40	23.21169942698102	25.483074920654275	27.39736806845886	23.907857583905844
41	16.43635079849057	23.94755703073659	29.720494626750764	29.895597544022078
42	21.429477831758	24.57409937196893	26.49165354576318	27.50476925050989
43	21.914732677394497	26.118549637423534	27.030573536490525	24.93614414869144
44	18.613652781548833	30.954509396362823	30.17604577913355	20.255792042954802
45	16.077070804672342	39.22829946710582	23.293366860461408	21.401262867760433
46	21.939686615402394	32.896521265079585	27.34972873226197	17.814063387256052
47	20.903389269381307	27.91544584960006	27.692561812393173	23.48860306862546
48	23.3062691806814	22.84547202996741	32.63415045664998	21.214108332701215
49	21.114079905061608	21.64371306090533	35.178317863545914	22.063889170487148
50	19.072535851790622	33.22631024125212	27.78862029534971	19.912533611607554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	509.0
1	947.0
2	1385.0
3	12848.0
4	24311.0
5	18752.0
6	13193.0
7	14106.0
8	15019.0
9	16291.0
10	17563.0
11	17484.0
12	17405.0
13	16545.5
14	15686.0
15	14268.5
16	12851.0
17	11741.0
18	10631.0
19	9882.0
20	9133.0
21	8395.0
22	7657.0
23	7528.5
24	7400.0
25	8765.0
26	10130.0
27	9655.5
28	9181.0
29	11685.0
30	14189.0
31	17738.5
32	21288.0
33	23209.0
34	25130.0
35	31894.5
36	38659.0
37	39654.0
38	40649.0
39	55986.5
40	71324.0
41	99240.5
42	127157.0
43	148459.0
44	169761.0
45	164892.0
46	160023.0
47	145744.0
48	131465.0
49	119307.0
50	107149.0
51	100300.5
52	93452.0
53	84391.0
54	75330.0
55	62403.0
56	49476.0
57	42563.5
58	35651.0
59	30770.5
60	25890.0
61	22411.0
62	18932.0
63	15540.0
64	12148.0
65	9966.0
66	7784.0
67	6522.5
68	5261.0
69	4037.5
70	2814.0
71	2372.0
72	1930.0
73	1709.5
74	1489.0
75	1199.5
76	910.0
77	614.0
78	318.0
79	262.5
80	207.0
81	161.0
82	115.0
83	70.0
84	25.0
85	15.5
86	6.0
87	6.0
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1410599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.185394017234614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31449882056316	15.600506991864634
2	6.91155184450511	2.652016908147504
3	2.3086062935016445	1.328745641144899
4	1.194482067524936	0.9166643644794777
5	0.7571938077884678	0.7263530774915985
6	0.5571244849749362	0.6413191656535833
7	0.42750170609649507	0.5741252072150901
8	0.3535367486416646	0.5426193457809897
9	0.29096695179773463	0.5024084054609925
>10	3.316770229528215	14.691513473307097
>50	1.1638996577736027	16.272652922378324
>100	1.399375011664673	43.81158525337941
>500	0.003743646366144123	0.4256250571099023
>1k	3.743646366144123E-4	0.31135413813220153
>5k	0.0	0.0
>10k+	3.743646366144123E-4	1.0025100484542722
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13958	0.9895087122562827	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4335	0.3073162535915593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04941163293040758	0.0
2	0.0	0.0	0.0	0.2018291520127265	0.0
3	0.0	0.0	0.0	0.27321726443872424	0.0
4	0.0	0.0	0.0	0.36863772057119	0.0
5	0.0	0.0	0.0	0.5720264937094099	0.0
6	0.0	0.0	0.0	0.7369209817956769	0.0
7	0.0	0.0	0.0	0.8396433004702257	0.0
8	0.0	0.0	0.0	1.0703963351739225	0.0
9	0.0	0.0	0.0	1.1639736027035323	0.0
10	0.0	0.0	0.0	1.3548854068378045	0.0
11	0.0	0.0	0.0	1.5635910701765703	0.0
12	0.0	0.0	0.0	1.7472719036381	0.0
13	0.0	0.0	0.0	1.8260327704755215	0.0
14	0.0	0.0	0.0	1.855807355598579	0.0
15	0.0	0.0	0.0	1.9115283649003012	0.0
16	0.0	0.0	0.0	2.0338877313822	0.0
17	0.0	0.0	0.0	2.1893536008461654	0.0
18	0.0	0.0	0.0	2.414506177871954	0.0
19	0.0	0.0	0.0	2.5044679600651922	0.0
20	0.0	0.0	0.0	2.6040001446194134	0.0
21	0.0	0.0	0.0	2.7179233786497794	0.0
22	0.0	0.0	0.0	2.8375179622273943	0.0
23	0.0	0.0	0.0	2.986887130928067	0.0
24	0.0	0.0	0.0	3.0876244772610786	0.0
25	0.0	0.0	0.0	3.1694336944801464	0.0
26	0.0	0.0	0.0	3.2578358555478912	0.0
27	0.0	0.0	0.0	3.345954449138274	0.0
28	0.0	0.0	0.0	3.437121393110303	0.0
29	0.0	0.0	0.0	3.543459197121223	0.0
30	0.0	0.0	0.0	3.676594127742895	0.0
31	0.0	0.0	0.0	3.7966849544058943	0.0
32	0.0	0.0	0.0	3.9060711087984608	0.0
33	0.0	0.0	0.0	4.007021130739495	0.0
34	0.0	0.0	0.0	4.120589905423157	0.0
35	0.0	0.0	0.0	4.266839831872843	0.0
36	0.0	0.0	0.0	4.385371037410349	0.0
37	0.0	0.0	0.0	4.510211619319168	0.0
38	0.0	0.0	0.0	4.617471017631517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	20	7.85709E-4	44.000004	42
ATCTCGC	40	8.314601E-9	44.000004	11
TCGCAGA	20	7.85709E-4	44.000004	12
CGACGGA	20	7.85709E-4	44.000004	3
CGACCCA	20	7.85709E-4	44.000004	20
GTTAGTA	20	7.85709E-4	44.000004	18
ATCACGC	20	7.85709E-4	44.000004	24
ATATCGC	20	7.85709E-4	44.000004	36
AGTGCCG	20	7.85709E-4	44.000004	1
GTTCGGC	20	7.85709E-4	44.000004	32
GTTCGCT	20	7.85709E-4	44.000004	17
CTACGCG	20	7.85709E-4	44.000004	1
CGCTAGC	20	7.85709E-4	44.000004	20
ACGTGAC	20	7.85709E-4	44.000004	31
CGTACGA	20	7.85709E-4	44.000004	40
TGCTCGA	20	7.85709E-4	44.000004	42
CTCTACG	20	7.85709E-4	44.000004	40
TCGGTTA	40	8.314601E-9	44.000004	25
AATACGT	20	7.85709E-4	44.000004	33
CGCCAGT	40	8.314601E-9	44.000004	26
>>END_MODULE