FastQCFastQC Report
Thu 26 May 2016
SRR1545929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545929_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences694096
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86401.2447845831124225No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11120.16020838615983954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG254.441012E-544.02
TATCACG207.8543235E-444.01
GGTACGC207.8543235E-444.08
ACGTTAG207.8543235E-444.01
TCGATTG207.8543235E-444.01
CCGCTCG207.8543235E-444.019
TATGACG207.8543235E-444.01
GTTACGG750.044.02
CCCGTAG207.8543235E-444.035
TACGATG408.303687E-944.01
TATAGCG302.5262616E-644.01
ACGTAAG207.8543235E-444.01
CGATAAC207.8543235E-444.010
TGTTACG351.4449688E-743.9999961
TACGTAG351.4449688E-743.9999961
CGTTTTT53150.043.0479771
TCACGAC603.6379788E-1240.33333225
TACGGGA1200.040.3333324
CGGTCTA603.6379788E-1240.33333231
ATACTCG452.3486791E-839.1111144
Produced by FastQC (version 0.11.5)