##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545929_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	694096
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512777771374566	31.0	31.0	33.0	30.0	34.0
2	31.915765254374033	31.0	31.0	34.0	30.0	34.0
3	31.94381036628939	31.0	31.0	34.0	30.0	34.0
4	35.72648740231899	37.0	35.0	37.0	35.0	37.0
5	35.670724222585925	37.0	35.0	37.0	33.0	37.0
6	35.72470810954104	37.0	35.0	37.0	33.0	37.0
7	35.993894216362	37.0	35.0	37.0	35.0	37.0
8	35.98312049053733	37.0	35.0	37.0	35.0	37.0
9	37.78996997533483	39.0	38.0	39.0	35.0	39.0
10	37.27134142827505	39.0	37.0	39.0	34.0	39.0
11	36.96122582466979	39.0	37.0	39.0	33.0	39.0
12	36.19486353472718	38.0	35.0	39.0	32.0	39.0
13	35.89134932343653	38.0	35.0	39.0	32.0	39.0
14	36.850347790507364	39.0	35.0	41.0	31.0	41.0
15	37.03795440400175	39.0	35.0	41.0	32.0	41.0
16	37.20679560176114	39.0	35.0	41.0	32.0	41.0
17	37.144778243931675	39.0	35.0	41.0	32.0	41.0
18	37.00389571471381	38.0	35.0	40.0	32.0	41.0
19	36.837794771904754	38.0	35.0	40.0	32.0	41.0
20	36.59985506327655	38.0	35.0	40.0	32.0	41.0
21	36.473957492911644	37.0	35.0	40.0	31.0	41.0
22	36.36651558285885	37.0	35.0	40.0	31.0	41.0
23	36.30775569949978	37.0	35.0	40.0	31.0	41.0
24	36.32697206150158	37.0	35.0	40.0	31.0	41.0
25	36.29597923053871	37.0	35.0	40.0	31.0	41.0
26	36.073705942693806	37.0	35.0	40.0	31.0	41.0
27	36.06691581567968	37.0	35.0	40.0	31.0	41.0
28	36.049081106936214	37.0	35.0	40.0	31.0	41.0
29	35.969223277471706	37.0	35.0	40.0	31.0	41.0
30	35.8420232936078	36.0	35.0	40.0	30.0	41.0
31	35.57837964777207	36.0	35.0	40.0	30.0	41.0
32	35.295364041861646	36.0	34.0	40.0	29.0	41.0
33	34.99525714022268	36.0	34.0	40.0	27.0	41.0
34	34.70234809017773	36.0	34.0	40.0	24.0	41.0
35	34.41811795486503	36.0	34.0	40.0	23.0	41.0
36	34.21888470759088	36.0	33.0	40.0	22.0	41.0
37	34.117702738526084	36.0	33.0	40.0	22.0	41.0
38	34.02422431479219	36.0	33.0	40.0	22.0	41.0
39	33.92848107466402	36.0	33.0	40.0	21.0	41.0
40	33.761747654503125	36.0	33.0	40.0	21.0	41.0
41	33.713695223715455	36.0	33.0	40.0	20.0	41.0
42	33.67994917129619	35.0	33.0	40.0	20.0	41.0
43	33.58073955187755	35.0	33.0	40.0	20.0	41.0
44	33.43711100481778	35.0	33.0	40.0	19.0	41.0
45	33.33545359719693	35.0	33.0	40.0	20.0	41.0
46	33.294316636315436	35.0	33.0	40.0	20.0	41.0
47	33.245857921670776	35.0	33.0	40.0	20.0	41.0
48	33.12846637929047	35.0	33.0	40.0	19.0	41.0
49	33.08920091745234	35.0	33.0	39.0	18.0	41.0
50	32.938717410848064	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	6.0
14	11.0
15	24.0
16	57.0
17	135.0
18	324.0
19	682.0
20	1259.0
21	2024.0
22	3080.0
23	4618.0
24	6814.0
25	10001.0
26	14643.0
27	17475.0
28	18078.0
29	17802.0
30	18711.0
31	21363.0
32	26075.0
33	33944.0
34	62244.0
35	100712.0
36	45303.0
37	57430.0
38	87009.0
39	144267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.99586800673106	22.18468338673613	27.340598418662548	17.478850187870265
2	36.09947327170882	23.769478573569074	28.22001567506512	11.911032479656992
3	28.24623683179272	23.705222332357483	35.757445655932315	12.291095179917475
4	25.21682879601669	25.857518268366338	34.890274544155275	14.0353783914617
5	22.219548880846453	30.535401443028054	33.37045595998248	13.874593716143014
6	19.811092413729515	39.26891957308499	31.084605011410527	9.835383001774971
7	84.42261589174984	3.2616525667919136	10.91621331919504	1.3995182222632028
8	85.70802886056107	2.2927664184781356	10.762488186072243	1.2367165348885456
9	82.50861552292477	3.482515386920541	11.871844816855305	2.137024273299371
10	48.96282358636269	22.768176160070077	17.459976717917982	10.809023535649247
11	42.644101104170026	20.31217583734815	23.870185104077823	13.173537954404003
12	38.69219819736751	19.938164173255572	27.831884926580763	13.537752702796155
13	22.872340425531913	33.62978608146424	28.11830063852839	15.379572854475462
14	17.36171941633434	34.67848827827851	32.04715197897697	15.91264032641018
15	16.400036882506168	21.43882114289666	46.320249648463616	15.84089232613356
16	19.3329164841751	16.895069269956895	44.45480164127152	19.31721260459648
17	19.789913787141835	17.657643899402963	31.73840506212397	30.81403725133123
18	23.226614185933933	21.558112998778267	36.09154929577465	19.12372351951315
19	28.107783361379408	22.936452594453794	28.7741177012978	20.181646342868998
20	29.424604089347874	22.084265000806806	28.86214587031189	19.628985039533436
21	23.58765934395242	25.078375325603375	31.904664484451718	19.429300845992486
22	23.2655137041562	21.74944099951592	30.511053226066707	24.473992070261176
23	20.378881307484846	26.872363477098272	29.3152532214564	23.43350199396049
24	21.71457550540559	21.6336068785874	38.67346879970494	17.978348816302066
25	19.76052304004057	22.881993268942622	35.85296558401143	21.50451810700537
26	18.030647057467554	28.707268158871397	30.492611972983564	22.769472810677485
27	17.637473778843272	28.57673866439224	33.31599663447131	20.469790922293168
28	16.543676955349117	25.678868629123347	38.90153523431918	18.875919181208364
29	17.946364767985983	22.90864664253936	37.9219877365667	21.223000852907955
30	20.676102441160875	26.519242295013946	34.02022198658399	18.784433277241188
31	27.79975680597497	24.036876743274703	28.61304488139969	19.550321569350636
32	28.473726977248106	24.33280122634333	28.924385099467507	18.269086696941056
33	26.787793043037272	25.016136096447756	28.43209008552131	19.763980774993662
34	20.22068993338097	26.08644913671884	31.05175652935617	22.64110440054402
35	20.806920080219452	25.15271662709481	32.37304349830571	21.667319794380028
36	28.35962172379613	25.216396579147553	27.205602683202322	19.21837901385399
37	21.204559599824808	30.219018694820317	28.982734376800906	19.593687328553973
38	22.098095940619164	29.831752380074228	26.08284732947604	21.987304349830573
39	21.095640948802473	28.50599917014361	27.949447914985825	22.448911966068096
40	24.61316590212305	24.96499043359996	26.96154422443005	23.46029943984694
41	19.28450819483184	23.66344136834098	29.178816763099054	27.87323367372813
42	22.334374495746985	25.295348193909778	25.87365436481409	26.496622945529147
43	22.518066665129897	25.67901270141306	27.06369147783592	24.739229155621125
44	21.063512828196675	27.22000991217353	29.458605149719926	22.25787210990987
45	19.490531569120122	32.550540559231	25.26883889260275	22.690088979046127
46	22.365494110324796	29.12017934118623	27.893547866577535	20.620778681911435
47	21.975922638943317	26.835336898642264	29.28845577556995	21.900284686844472
48	21.984711048615754	24.745856480947882	31.337595952144948	21.931836518291416
49	21.854325626426316	24.16005855097854	31.81966183352159	22.165953989073557
50	21.15427837071529	28.94354671399922	28.542593531730482	21.359581383555014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	702.0
2	1192.0
3	8383.5
4	15575.0
5	10917.0
6	6259.0
7	6195.0
8	6131.0
9	6205.0
10	6279.0
11	6076.5
12	5874.0
13	5513.0
14	5152.0
15	4751.5
16	4351.0
17	4019.0
18	3687.0
19	3380.5
20	3074.0
21	3063.5
22	3053.0
23	3108.0
24	3163.0
25	3581.0
26	3999.0
27	4761.5
28	5524.0
29	5845.0
30	6166.0
31	8075.0
32	9984.0
33	11554.0
34	13124.0
35	14618.0
36	16112.0
37	17577.0
38	19042.0
39	23203.5
40	27365.0
41	35658.0
42	43951.0
43	50432.0
44	56913.0
45	58324.5
46	59736.0
47	56057.5
48	52379.0
49	52375.0
50	52371.0
51	48684.5
52	44998.0
53	42103.0
54	39208.0
55	37158.5
56	35109.0
57	33310.5
58	31512.0
59	30987.5
60	30463.0
61	27924.0
62	25385.0
63	22010.0
64	18635.0
65	16073.0
66	13511.0
67	11193.0
68	8875.0
69	7387.0
70	5899.0
71	5162.5
72	4426.0
73	3424.5
74	2423.0
75	1956.0
76	1489.0
77	1154.5
78	820.0
79	550.0
80	280.0
81	239.0
82	198.0
83	153.0
84	108.0
85	75.5
86	43.0
87	27.5
88	12.0
89	9.0
90	6.0
91	12.0
92	18.0
93	12.5
94	7.0
95	4.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	694096.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.0379344406389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8389776788397	18.73813416727226
2	6.916714620904456	3.4636049444562853
3	2.993753162643713	2.248721862531859
4	1.890879651194802	1.893748829670144
5	1.2863241331547102	1.6103449657669646
6	1.022115315750463	1.5354993759919813
7	0.8184165950489496	1.434402273637656
8	0.6942287605792418	1.3905643355351243
9	0.5715896166481559	1.2880281013727801
>10	7.879029442791606	46.6387460643303
>50	1.0257514075850298	15.994467057295447
>100	0.060475139676214866	2.2702397191294845
>500	5.814917276559121E-4	0.07367031987940224
>1k	5.814917276559121E-4	0.16189999151362708
>5k	5.814917276559121E-4	1.2579279916166708
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8640	1.2447845831124225	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1112	0.16020838615983954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03198404831608308	0.0
2	0.0	0.0	0.0	0.13341094027339157	0.0
3	0.0	0.0	0.0	0.18787026578456006	0.0
4	0.0	0.0	0.0	0.2439143864825615	0.0
5	0.0	0.0	0.0	0.39187662801687373	0.0
6	0.0	0.0	0.0	0.5143380742721468	0.0
7	0.0	0.0	0.0	0.5938659781932182	0.0
8	0.0	0.0	0.0	0.7447096655217722	0.0
9	0.0	0.0	0.0	0.8069488946773933	0.0
10	0.0	0.0	0.0	0.9296984855120906	0.0
11	0.0	0.0	0.0	1.1076277633065166	0.0
12	0.0	0.0	0.0	1.2498271132523455	0.0
13	0.0	0.0	0.0	1.3114900532491183	0.0
14	0.0	0.0	0.0	1.3414570895092321	0.0
15	0.0	0.0	0.0	1.3806447523109195	0.0
16	0.0	0.0	0.0	1.4748680297826238	0.0
17	0.0	0.0	0.0	1.5803289458518706	0.0
18	0.0	0.0	0.0	1.7304522717318642	0.0
19	0.0	0.0	0.0	1.8007595491113622	0.0
20	0.0	0.0	0.0	1.8697701758834513	0.0
21	0.0	0.0	0.0	1.9652901039625643	0.0
22	0.0	0.0	0.0	2.0616744657799497	0.0
23	0.0	0.0	0.0	2.174338996334801	0.0
24	0.0	0.0	0.0	2.261502731610613	0.0
25	0.0	0.0	0.0	2.3315218644106865	0.0
26	0.0	0.0	0.0	2.4073038887992437	0.0
27	0.0	0.0	0.0	2.4902895276734056	0.0
28	0.0	0.0	0.0	2.5689529978562042	0.0
29	0.0	0.0	0.0	2.6584218897674097	0.0
30	0.0	0.0	0.0	2.7692134805560036	0.0
31	0.0	0.0	0.0	2.869920011064752	0.0
32	0.0	0.0	0.0	2.9566515294714275	0.0
33	0.0	0.0	0.0	3.04799336114889	0.0
34	0.0	0.0	0.0	3.1540305663769854	0.0
35	0.0	0.0	0.0	3.289314446416634	0.0
36	0.0	0.0	0.0	3.3852665913648834	0.0
37	0.0	0.0	0.0	3.490871579723842	0.0
38	0.0	0.0	0.0	3.609587146446601	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	25	4.441012E-5	44.0	2
TATCACG	20	7.8543235E-4	44.0	1
GGTACGC	20	7.8543235E-4	44.0	8
ACGTTAG	20	7.8543235E-4	44.0	1
TCGATTG	20	7.8543235E-4	44.0	1
CCGCTCG	20	7.8543235E-4	44.0	19
TATGACG	20	7.8543235E-4	44.0	1
GTTACGG	75	0.0	44.0	2
CCCGTAG	20	7.8543235E-4	44.0	35
TACGATG	40	8.303687E-9	44.0	1
TATAGCG	30	2.5262616E-6	44.0	1
ACGTAAG	20	7.8543235E-4	44.0	1
CGATAAC	20	7.8543235E-4	44.0	10
TGTTACG	35	1.4449688E-7	43.999996	1
TACGTAG	35	1.4449688E-7	43.999996	1
CGTTTTT	5315	0.0	43.047977	1
TCACGAC	60	3.6379788E-12	40.333332	25
TACGGGA	120	0.0	40.333332	4
CGGTCTA	60	3.6379788E-12	40.333332	31
ATACTCG	45	2.3486791E-8	39.11111	44
>>END_MODULE