FastQCFastQC Report
Thu 26 May 2016
SRR1545928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545928_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1494265
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119090.7969804552739976No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29590.1980237775762666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC302.5282297E-644.021
CGACAAC207.857242E-444.034
TAGCACG302.5282297E-644.01
CGTAACG207.857242E-444.034
CACGTAT254.4434833E-544.039
GTAACGT207.857242E-444.035
GCGTAAG1000.044.01
TACACCG254.4434833E-544.01
TGCGTAA207.857242E-444.032
CGTTTTT82200.043.063261
CGGTCTA1900.042.84210631
ATTAGCG900.041.5555571
TAGGGTC2850.040.9122775
TGTTACG700.040.8571431
TAATGCG650.040.6153831
TGCGAAG1750.040.228571
GTTACGG2100.039.8095252
CTAACGG452.3517714E-839.111112
TGGGCGA5200.038.9230776
ACGGGAC2100.038.7619065
Produced by FastQC (version 0.11.5)