FastQCFastQC Report
Thu 26 May 2016
SRR1545927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545927_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences970826
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115491.1896055523852884No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25570.2633839637586962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG302.5273075E-644.01
AACGTAG551.8189894E-1244.01
AAACTCG207.855878E-444.01
CTATGCG351.4457692E-744.01
AATCCGG254.4423276E-544.02
GCAAGCG351.4457692E-744.01
CATATTG302.5273075E-644.01
CGTATGA351.4457692E-744.036
CTGTACG351.4457692E-744.01
GTCGAAT207.855878E-444.022
GTACACG207.855878E-444.01
ACGTTCA254.4423276E-544.044
ACGTTAG207.855878E-444.01
GGGTATA302.5273075E-644.07
CGCATCG207.855878E-444.021
GCACGAC207.855878E-444.038
CGTCTTA207.855878E-444.022
CGCGATC207.855878E-444.042
CTAAGCG207.855878E-444.01
CACGAGG551.8189894E-1244.02
Produced by FastQC (version 0.11.5)