##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545927_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	970826
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551108025537015	31.0	31.0	33.0	30.0	34.0
2	31.943401804236803	31.0	31.0	34.0	30.0	34.0
3	31.936032821535477	33.0	31.0	34.0	30.0	34.0
4	35.71677622972603	37.0	35.0	37.0	33.0	37.0
5	35.67065261952193	37.0	35.0	37.0	33.0	37.0
6	35.734280911306456	37.0	35.0	37.0	33.0	37.0
7	36.00092395547709	37.0	35.0	37.0	35.0	37.0
8	35.99017228627993	37.0	35.0	37.0	35.0	37.0
9	37.819760698621586	39.0	38.0	39.0	35.0	39.0
10	37.362706602418974	39.0	37.0	39.0	35.0	39.0
11	36.969110839635526	39.0	37.0	39.0	33.0	39.0
12	35.66319402240978	37.0	35.0	39.0	32.0	39.0
13	35.12657572005694	35.0	35.0	39.0	30.0	39.0
14	36.013660532371404	37.0	35.0	41.0	31.0	41.0
15	36.368281236802474	37.0	35.0	41.0	32.0	41.0
16	36.65219926124764	37.0	35.0	41.0	32.0	41.0
17	36.58976170807127	36.0	35.0	41.0	32.0	41.0
18	36.461383399290916	36.0	35.0	40.0	32.0	41.0
19	36.22693458972051	36.0	35.0	40.0	32.0	41.0
20	35.921744988288324	35.0	35.0	40.0	32.0	41.0
21	35.71336573186132	35.0	35.0	40.0	31.0	41.0
22	35.58572287928012	35.0	35.0	40.0	31.0	41.0
23	35.59817825233358	35.0	35.0	40.0	31.0	41.0
24	35.59013973667784	35.0	35.0	40.0	31.0	41.0
25	35.545223345893085	35.0	35.0	40.0	31.0	41.0
26	35.28750981123291	35.0	35.0	40.0	31.0	41.0
27	35.24046327560242	35.0	34.0	40.0	30.0	41.0
28	35.24466073220124	35.0	34.0	40.0	30.0	41.0
29	35.23934155039111	35.0	35.0	40.0	30.0	41.0
30	35.137341809963885	35.0	35.0	40.0	30.0	41.0
31	34.76309142936015	35.0	34.0	40.0	29.0	41.0
32	34.352978803616715	35.0	34.0	40.0	27.0	41.0
33	33.9676584681498	35.0	33.0	40.0	23.0	41.0
34	33.66475454921891	35.0	34.0	40.0	23.0	41.0
35	33.361813548462855	35.0	33.0	39.0	21.0	41.0
36	33.08793027792828	35.0	33.0	39.0	18.0	41.0
37	32.97111943849876	35.0	33.0	39.0	18.0	41.0
38	32.95601786519933	35.0	33.0	39.0	18.0	41.0
39	32.8950131125454	35.0	33.0	39.0	18.0	41.0
40	32.674247496461774	35.0	32.0	39.0	18.0	41.0
41	32.692194069792116	35.0	33.0	39.0	18.0	41.0
42	32.61183466450219	35.0	32.0	39.0	18.0	41.0
43	32.460911636070726	35.0	32.0	39.0	17.0	41.0
44	32.33325539283044	35.0	32.0	38.0	16.0	41.0
45	32.262022236734495	35.0	32.0	38.0	17.0	41.0
46	32.246176966830305	35.0	32.0	38.0	17.0	41.0
47	32.193199399274434	35.0	32.0	38.0	17.0	41.0
48	32.142795928415595	35.0	32.0	38.0	15.0	41.0
49	32.144255510256215	35.0	32.0	38.0	15.0	40.0
50	31.9158520682388	35.0	32.0	37.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	8.0
15	36.0
16	85.0
17	234.0
18	536.0
19	1091.0
20	2149.0
21	3291.0
22	5077.0
23	7561.0
24	11597.0
25	17206.0
26	24655.0
27	29682.0
28	29264.0
29	27617.0
30	29058.0
31	32397.0
32	39033.0
33	52752.0
34	107639.0
35	204638.0
36	49544.0
37	58075.0
38	86698.0
39	150902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.44373966086611	21.80565827450027	27.502765686127074	19.24783637850655
2	35.9801859447522	23.690136028495324	28.45885874502743	11.870819281725046
3	27.129475312774893	23.323953004966906	36.81298193497084	12.733589747287361
4	23.790462966587214	25.51126566449601	36.641066473291815	14.057204895624963
5	21.403011456223876	30.6533817594502	35.34680776987844	12.596799014447491
6	18.81521508488648	38.41213564531646	32.52776501659412	10.244884253202944
7	82.49356733338415	3.8292134738871844	12.371835941765054	1.3053832509636125
8	84.90501902503641	2.2616823200037905	11.881222793785911	0.952075861173887
9	82.46029669580336	3.044520851316302	12.822277112479478	1.6729053404008547
10	60.11252273836918	16.033151151699688	16.463815348991478	7.390510760939653
11	55.510565230020624	14.245498163419605	21.077927455589364	9.16600915097041
12	48.43586801342362	18.531127102075963	23.86555366255127	9.167451221949143
13	18.311211277819094	46.77923747406847	24.317230894104608	10.592320354007825
14	11.869686225956041	46.92365058208165	30.291524948857983	10.915138243104327
15	10.399288852997344	18.653909145408136	59.8914738583433	11.055328143251211
16	11.74443206094604	12.888303362291492	57.50052017560304	17.866744401159426
17	12.524489455370993	13.84697154793959	32.780230442942404	40.84830855374702
18	21.45585305708747	19.571375303092417	39.42158533042996	19.551186309390147
19	29.29783503944064	21.143541685121743	29.69327150282337	19.865351772614247
20	32.79125198542272	19.484542029158675	28.297140785269452	19.42706520014915
21	20.269646672009195	25.976333555137582	32.492434277615146	21.261585495238077
22	21.0405366152122	21.438342195202846	30.010115097865114	27.511006091719835
23	17.385710724681868	28.32196500711765	28.315578692783262	25.97674557541722
24	19.031113711416875	19.67489539835151	45.45994853866707	15.834042351564545
25	13.630454890989736	21.05845949737646	43.75758374827209	21.553501863361717
26	13.745202538868964	34.6565707964146	32.440416717310825	19.15780994740561
27	15.431395533288148	33.58974728736148	34.64936044152093	16.329496737829434
28	11.819007731560548	26.850640588529767	46.66150267916187	14.668849000747816
29	12.275011176050086	20.927334043381617	46.316332689895	20.481322090673302
30	16.024395720757376	28.404884088394834	38.39802395073886	17.17269624010894
31	31.44734483831294	23.86730474873973	26.95117353676148	17.734176876185845
32	31.253283286603367	25.11655023660265	29.106142604338984	14.524023872455002
33	28.1993889739253	27.259982736350285	26.86361922733837	17.677009062386052
34	18.082024997270366	27.542731653251973	30.2064427611127	24.16880058836496
35	17.968307400090232	25.596347852241287	33.49096542531824	22.944379322350247
36	32.496451475341615	21.953676559960282	28.85862142134636	16.691250543351742
37	19.224660237776902	31.781493285099494	31.49925939354735	17.494587083576256
38	19.503803977231758	32.96666961947867	24.50799628357708	23.02153011971249
39	19.881935588869684	31.09733361076032	29.557819835892325	19.462910964477672
40	23.70424772307293	25.551849662040365	26.320885513984997	24.423017100901706
41	16.405514479422678	22.735279030433876	29.467896409861293	31.391310080282153
42	23.883991570065078	23.45034022574591	24.76107974034482	27.90458846384419
43	23.476194498293207	25.304534489187557	25.389616676932842	25.8296543355864
44	18.322953855788782	31.732462871822552	28.855325259109254	21.089258013279412
45	15.710642277812914	40.62622962302205	22.044527031620493	21.618601067544542
46	22.512891084499177	32.99540803398343	26.15442932101118	18.337271560506206
47	21.669279561940037	27.01153450772847	27.713205043952264	23.60598088637923
48	24.736976553985986	21.99024335977817	31.916635936820807	21.356144149415034
49	20.572172562333517	21.87106649389283	33.86610988992878	23.690651053844867
50	18.91791113958629	33.221813177644606	27.813634987114067	20.04664069565504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	461.0
1	861.5
2	1262.0
3	10676.0
4	20090.0
5	14929.0
6	9768.0
7	10487.0
8	11206.0
9	11870.5
10	12535.0
11	12260.5
12	11986.0
13	11157.5
14	10329.0
15	9301.0
16	8273.0
17	7413.5
18	6554.0
19	5945.0
20	5336.0
21	4737.0
22	4138.0
23	4134.0
24	4130.0
25	4252.0
26	4374.0
27	4310.0
28	4246.0
29	5861.5
30	7477.0
31	9017.5
32	10558.0
33	11559.5
34	12561.0
35	13527.0
36	14493.0
37	15414.5
38	16336.0
39	27521.5
40	38707.0
41	63025.0
42	87343.0
43	102444.0
44	117545.0
45	115283.5
46	113022.0
47	97498.5
48	81975.0
49	77658.5
50	73342.0
51	67427.0
52	61512.0
53	56866.5
54	52221.0
55	43883.5
56	35546.0
57	33407.0
58	31268.0
59	28347.0
60	25426.0
61	24021.0
62	22616.0
63	20283.5
64	17951.0
65	14873.0
66	11795.0
67	10224.0
68	8653.0
69	7239.0
70	5825.0
71	4987.5
72	4150.0
73	3321.5
74	2493.0
75	2049.5
76	1606.0
77	1226.0
78	846.0
79	659.0
80	472.0
81	359.5
82	247.0
83	157.0
84	67.0
85	46.0
86	25.0
87	23.5
88	22.0
89	16.5
90	11.0
91	9.5
92	8.0
93	13.0
94	18.0
95	9.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	970826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.816620347432504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42635757281809	17.32814688745593
2	7.67725289535058	3.3498342345818113
3	2.967291075002504	1.942087885309635
4	1.6429891529883878	1.4337788234278943
5	1.0724138171434965	1.1698222551980284
6	0.7375190516321818	0.9654103889074669
7	0.505441080282163	0.7718911309558474
8	0.37210133172226767	0.6494394787967006
9	0.2658830415896213	0.5220602437663238
>10	2.8366461700993013	15.30870254937512
>50	1.4260725171615063	22.48619520986997
>100	1.0681206688518115	32.49408891503872
>500	9.558126790620236E-4	0.10780795410999355
>1k	4.779063395310118E-4	0.2666005209470537
>5k	0.0	0.0
>10k+	4.779063395310118E-4	1.2041335222594929
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11549	1.1896055523852884	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2557	0.2633839637586962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0300506990954095E-4	0.0	0.0	0.036257784608158414	0.0
2	1.0300506990954095E-4	0.0	0.0	0.10897936396429432	0.0
3	1.0300506990954095E-4	0.0	0.0	0.15182947304666336	0.0
4	1.0300506990954095E-4	0.0	0.0	0.21311748964284022	0.0
5	1.0300506990954095E-4	0.0	0.0	0.3652559778992322	0.0
6	1.0300506990954095E-4	0.0	0.0	0.4834027930854757	0.0
7	1.0300506990954095E-4	0.0	0.0	0.5592145245388978	0.0
8	1.0300506990954095E-4	0.0	0.0	0.7446236503760715	0.0
9	1.0300506990954095E-4	0.0	0.0	0.8081777785102583	0.0
10	1.0300506990954095E-4	0.0	0.0	0.9665995760311322	0.0
11	1.0300506990954095E-4	0.0	0.0	1.110909678974399	0.0
12	1.0300506990954095E-4	0.0	0.0	1.259030969504319	0.0
13	1.0300506990954095E-4	0.0	0.0	1.3158897680943855	0.0
14	1.0300506990954095E-4	0.0	0.0	1.335563736447108	0.0
15	7.210354893667866E-4	0.0	0.0	1.374396647803005	0.0
16	7.210354893667866E-4	0.0	0.0	1.4478392626485075	0.0
17	7.210354893667866E-4	0.0	0.0	1.5436339776643806	0.0
18	7.210354893667866E-4	0.0	0.0	1.7019527701153452	0.0
19	7.210354893667866E-4	0.0	0.0	1.757884523076226	0.0
20	7.210354893667866E-4	0.0	0.0	1.8169064281343927	0.0
21	7.210354893667866E-4	0.0	0.0	1.894469245776277	0.0
22	7.210354893667866E-4	0.0	0.0	1.9693539316005133	0.0
23	7.210354893667866E-4	0.0	0.0	2.0630885452181955	0.0
24	8.240405592763276E-4	0.0	0.0	2.125612622653287	0.0
25	8.240405592763276E-4	0.0	0.0	2.1820594009637153	0.0
26	8.240405592763276E-4	0.0	0.0	2.2428323922103446	0.0
27	8.240405592763276E-4	0.0	0.0	2.296601038703125	0.0
28	8.240405592763276E-4	0.0	0.0	2.35830107557894	0.0
29	8.240405592763276E-4	0.0	0.0	2.4158809096583735	0.0
30	8.240405592763276E-4	0.0	0.0	2.510439563835332	0.0
31	8.240405592763276E-4	0.0	0.0	2.6034531419636475	0.0
32	8.240405592763276E-4	0.0	0.0	2.6853421725417324	0.0
33	8.240405592763276E-4	0.0	0.0	2.758887792457145	0.0
34	8.240405592763276E-4	0.0	0.0	2.834905534050386	0.0
35	8.240405592763276E-4	0.0	0.0	2.965000937346136	0.0
36	8.240405592763276E-4	0.0	0.0	3.0595595915230946	0.0
37	8.240405592763276E-4	0.0	0.0	3.1467018806665665	0.0
38	8.240405592763276E-4	0.0	0.0	3.223028637469536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	30	2.5273075E-6	44.0	1
AACGTAG	55	1.8189894E-12	44.0	1
AAACTCG	20	7.855878E-4	44.0	1
CTATGCG	35	1.4457692E-7	44.0	1
AATCCGG	25	4.4423276E-5	44.0	2
GCAAGCG	35	1.4457692E-7	44.0	1
CATATTG	30	2.5273075E-6	44.0	1
CGTATGA	35	1.4457692E-7	44.0	36
CTGTACG	35	1.4457692E-7	44.0	1
GTCGAAT	20	7.855878E-4	44.0	22
GTACACG	20	7.855878E-4	44.0	1
ACGTTCA	25	4.4423276E-5	44.0	44
ACGTTAG	20	7.855878E-4	44.0	1
GGGTATA	30	2.5273075E-6	44.0	7
CGCATCG	20	7.855878E-4	44.0	21
GCACGAC	20	7.855878E-4	44.0	38
CGTCTTA	20	7.855878E-4	44.0	22
CGCGATC	20	7.855878E-4	44.0	42
CTAAGCG	20	7.855878E-4	44.0	1
CACGAGG	55	1.8189894E-12	44.0	2
>>END_MODULE