FastQCFastQC Report
Thu 26 May 2016
SRR1545926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545926_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1617412
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140410.8681152359448304No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27710.17132307661869703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG650.044.0000041
CGCTATC302.528359E-644.025
ACTAACG207.857434E-444.01
TACGTCC254.4436478E-544.034
ACGTTAG351.4465695E-743.9999961
CGTTTTT89200.042.766821
CGTAAGG2300.042.086962
AGTACGG1900.041.684212
CGGTCTA3450.040.81159231
TACGGGA3850.040.571434
ATAGCGG2550.040.549022
TAGCATA7800.040.0512830
TCGTTGA1100.040.024
ATCGTTG1100.040.023
CCGAACT557.8216544E-1140.012
TCGTCCC9400.039.7872338
ATACCGG501.3478711E-939.62
TATAGCG900.039.111111
ACGTAAG850.038.8235321
TACGGGT1200.038.54
Produced by FastQC (version 0.11.5)