FastQCFastQC Report
Thu 26 May 2016
SRR1545925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545925_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1675358
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158850.9481555583940865No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA51950.3100829792796525No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC254.4437154E-544.011
AATCCGC302.5284135E-644.023
CTCCGTC207.857515E-444.015
GTCGACA302.5284135E-644.013
CGACGTA207.857515E-444.039
CGCATCA254.4437154E-544.019
TCTTACG454.802132E-1044.01
TTACTCG302.5284135E-644.028
CGTCTAC254.4437154E-544.035
GCGCATC207.857515E-444.018
TCTAGCG600.044.01
GCGCACC207.857515E-444.021
ATCCGCG207.857515E-444.010
GTATGCG454.802132E-1044.01
TCTCGCA207.857515E-444.038
AGCGCAT254.4437154E-544.034
GCGATCG207.857515E-444.09
CCGACGG302.5284135E-644.02
CGTAACG207.857515E-444.011
TTCGGAC254.4437154E-544.033
Produced by FastQC (version 0.11.5)