##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545925_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1675358
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.516443052768423	31.0	31.0	33.0	30.0	34.0
2	31.901147098112762	31.0	31.0	34.0	30.0	34.0
3	31.893956993072525	31.0	31.0	34.0	30.0	34.0
4	35.67050445337653	37.0	35.0	37.0	33.0	37.0
5	35.613707637412425	37.0	35.0	37.0	33.0	37.0
6	35.67502826261611	37.0	35.0	37.0	33.0	37.0
7	36.01156230489244	37.0	35.0	37.0	35.0	37.0
8	36.01625861457671	37.0	35.0	37.0	35.0	37.0
9	37.827548500081775	39.0	38.0	39.0	35.0	39.0
10	37.31664038372694	39.0	37.0	39.0	34.0	39.0
11	36.94358937015253	39.0	37.0	39.0	33.0	39.0
12	35.86808431391977	37.0	35.0	39.0	32.0	39.0
13	35.38246989598641	37.0	35.0	39.0	31.0	39.0
14	36.297957809614424	38.0	35.0	41.0	31.0	41.0
15	36.617600536721106	38.0	35.0	41.0	32.0	41.0
16	36.86037133555933	38.0	35.0	41.0	32.0	41.0
17	36.81173576035689	38.0	35.0	41.0	32.0	41.0
18	36.680994151697725	38.0	35.0	40.0	32.0	41.0
19	36.480795746341975	37.0	35.0	40.0	32.0	41.0
20	36.21685395002143	36.0	35.0	40.0	32.0	41.0
21	36.032283846198844	35.0	35.0	40.0	31.0	41.0
22	35.91366681031756	35.0	35.0	40.0	31.0	41.0
23	35.894222607944094	35.0	35.0	40.0	31.0	41.0
24	35.90269124569197	35.0	35.0	40.0	31.0	41.0
25	35.858331174590745	35.0	35.0	40.0	31.0	41.0
26	35.60503963928903	35.0	35.0	40.0	31.0	41.0
27	35.582348966608926	35.0	35.0	40.0	31.0	41.0
28	35.59879798825087	36.0	35.0	40.0	31.0	41.0
29	35.602596579357964	36.0	35.0	40.0	31.0	41.0
30	35.475583129098375	36.0	35.0	40.0	30.0	41.0
31	35.166877168939415	35.0	34.0	40.0	29.0	41.0
32	34.780557946421006	35.0	34.0	40.0	27.0	41.0
33	34.41234231728383	35.0	34.0	40.0	24.0	41.0
34	34.096555482470016	35.0	34.0	40.0	23.0	41.0
35	33.80199933387372	35.0	33.0	40.0	22.0	41.0
36	33.5626552653224	35.0	33.0	40.0	20.0	41.0
37	33.43555466950944	35.0	33.0	40.0	19.0	41.0
38	33.39859659845836	35.0	33.0	40.0	19.0	41.0
39	33.346628601170615	35.0	33.0	40.0	18.0	41.0
40	33.15526233796	35.0	33.0	40.0	18.0	41.0
41	33.160881435490204	35.0	33.0	40.0	18.0	41.0
42	33.1320523732838	35.0	33.0	40.0	18.0	41.0
43	32.9865843598801	35.0	33.0	40.0	18.0	41.0
44	32.86705468323785	35.0	33.0	40.0	17.0	41.0
45	32.76454704009531	35.0	32.0	40.0	17.0	41.0
46	32.75246962141823	35.0	32.0	39.0	17.0	41.0
47	32.69349774794402	35.0	32.0	39.0	17.0	41.0
48	32.6118155045071	35.0	32.0	39.0	15.0	41.0
49	32.60461704304394	35.0	32.0	39.0	15.0	41.0
50	32.392683832351054	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	3.0
14	13.0
15	38.0
16	118.0
17	352.0
18	911.0
19	1810.0
20	3356.0
21	5374.0
22	8455.0
23	12609.0
24	19123.0
25	29078.0
26	41712.0
27	49203.0
28	47432.0
29	43953.0
30	46514.0
31	53231.0
32	65118.0
33	86346.0
34	168022.0
35	305916.0
36	88903.0
37	109401.0
38	172386.0
39	315976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.497268046590637	22.43371267514167	26.965341139028197	19.103678139239495
2	33.86422484030279	24.549499271200542	29.12380518074346	12.462470707753209
3	26.369707250629414	23.52500182050642	38.44730499391772	11.65798593494644
4	23.200414478577116	26.242808999628735	37.04366469733633	13.513111824457818
5	21.327919167127266	31.96021387667591	34.64608758247491	12.065779373721915
6	18.58361018958336	38.285906653980824	32.974325487448056	10.156157668987763
7	83.24817740447116	3.5540463590468425	12.102667012065481	1.0951092244165128
8	85.36444151041151	2.1513013934932115	11.418216285713262	1.0660408103820198
9	82.42739760695923	3.527604249360435	12.412929057550686	1.6320690861296512
10	52.64021182338342	23.53276135608031	16.489789048072115	7.337237772464154
11	49.11935240109875	16.56839911230913	25.02032401433007	9.291924472262048
12	44.46822708937433	18.933386177760216	26.228304636979082	10.370082095886373
13	19.60631697822197	43.552900335331316	25.92884625256214	10.911936433884579
14	13.343357061595192	44.578412494523555	30.551141905192804	11.527088538688448
15	11.757367679027409	20.99270723033525	55.34828973867077	11.901635351966565
16	12.632583603026934	15.927879295052161	54.30761664074186	17.131920461179043
17	13.636667506288209	16.13147757076398	32.93916882242482	37.29268610052299
18	19.95746580730805	21.12629061967651	39.41097962345958	19.505263949555857
19	26.678715832675763	21.94730917212918	31.4738700623986	19.900104932796452
20	31.071030788643384	20.359708193711434	29.170362394186792	19.39889862345839
21	20.34204032809704	26.290858431451664	33.215229222649725	20.151872017801566
22	21.125037156237653	22.275776281845435	30.798253268853582	25.800933293063334
23	16.874602323801838	29.12887872323408	29.228678288461328	24.767840664502753
24	18.44513232395703	21.494391049554782	44.70238599750024	15.358090628987952
25	14.486635095305003	22.585083307567697	42.64730284512325	20.28097875200405
26	14.30625573757967	34.33463176228603	32.52683903977538	18.83227346035892
27	15.140704255448686	34.27965843718179	33.818742024092764	16.76089528327677
28	12.335811211693262	27.330278065941727	45.3830166448007	14.950894077564318
29	12.791355638615748	21.461502556468528	44.87178262795176	20.87535917696397
30	16.100320050998054	29.056476287456174	37.64681936636826	17.19638429517751
31	28.10473940495106	25.118392606236995	29.35772533392863	17.419142654883316
32	28.918595309181676	26.57814031389112	29.883344335956853	14.619920040970346
33	26.802331203241337	28.333944148056712	27.888964627261753	16.974760021440193
34	18.25759031800964	28.184781998832488	31.194526781738592	22.363100901419276
35	18.413676360515186	26.398895042134278	33.361048802703664	21.826379794646876
36	29.93312474110011	22.962793623810555	29.78145566499817	17.322625970091167
37	19.629237452532532	30.92586778467647	31.797979894446442	17.646914868344556
38	19.559043499956427	31.348762473453434	26.919559879142245	22.17263414744789
39	19.75130091598333	31.101830175998202	29.838637473304214	19.30823143471425
40	23.256880022060958	26.689579182479207	26.961521059976434	23.0920197354834
41	17.069724799117562	24.422959152610964	29.813926336938135	28.69338971133334
42	23.613878347195048	25.122391751494305	25.301278890840045	25.9624510104706
43	23.092855377775972	26.631979553026873	26.40193916762865	23.8732259015685
44	19.930546187740173	31.594262241264254	28.114946178667484	20.36024539232809
45	16.4597059255395	39.09003329437649	22.642802314490396	21.80745846559362
46	22.749227329323045	32.542059667247244	26.88135908862464	17.827353914805073
47	22.341016069401288	28.2836265442968	27.293927626214813	22.081429760087097
48	23.744835432188225	23.2026826505141	31.006566954644914	22.04591496265276
49	21.10468329753999	22.666856874769454	33.46466844698268	22.763791380707886
50	18.778434221223165	32.53907523048805	28.116438397047077	20.566052151241703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	601.0
1	1213.0
2	1825.0
3	14950.5
4	28076.0
5	21943.5
6	15811.0
7	16574.5
8	17338.0
9	18508.0
10	19678.0
11	19441.0
12	19204.0
13	18150.5
14	17097.0
15	15856.0
16	14615.0
17	13367.0
18	12119.0
19	10981.0
20	9843.0
21	9033.5
22	8224.0
23	8530.5
24	8837.0
25	9071.0
26	9305.0
27	10673.5
28	12042.0
29	13461.5
30	14881.0
31	19229.5
32	23578.0
33	25738.5
34	27899.0
35	32546.5
36	37194.0
37	43947.0
38	50700.0
39	65176.0
40	79652.0
41	115829.0
42	152006.0
43	170518.0
44	189030.0
45	192338.0
46	195646.0
47	180129.0
48	164612.0
49	148833.5
50	133055.0
51	123922.0
52	114789.0
53	101553.0
54	88317.0
55	73639.5
56	58962.0
57	49594.0
58	40226.0
59	37076.5
60	33927.0
61	30665.0
62	27403.0
63	23074.5
64	18746.0
65	14838.0
66	10930.0
67	8774.5
68	6619.0
69	5834.0
70	5049.0
71	3978.5
72	2908.0
73	2409.0
74	1910.0
75	1683.5
76	1457.0
77	1085.5
78	714.0
79	458.0
80	202.0
81	161.5
82	121.0
83	100.0
84	79.0
85	74.0
86	69.0
87	48.5
88	28.0
89	26.0
90	24.0
91	14.0
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1675358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.04034185228973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.44839263758699	16.32253631776761
2	7.016979597820014	2.8124533982171114
3	2.300157801209683	1.3828784595135941
4	1.198276661389402	0.9605549571145618
5	0.7650433998984629	0.7665865632901597
6	0.5683864514823072	0.6834395275149194
7	0.43968910796083693	0.6168064022584447
8	0.3471043519649821	0.556487189743659
9	0.27234256641494103	0.4912054318696803
>10	3.1544960117708554	14.635179317885335
>50	1.159482893945433	16.883809952655515
>100	1.3233125487603274	41.91849882889399
>500	0.0057325441008732525	0.6949765036761683
>1k	0.0	0.0
>5k	3.017128474143817E-4	0.3141119659472502
>10k+	3.017128474143817E-4	0.9604751836519864
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15885	0.9481555583940865	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5195	0.3100829792796525	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05783838439306704	0.0
2	0.0	0.0	0.0	0.19189928361580033	0.0
3	0.0	0.0	0.0	0.26370483204186806	0.0
4	0.0	0.0	0.0	0.3701298468745188	0.0
5	0.0	0.0	0.0	0.6144955287168474	0.0
6	0.0	0.0	0.0	0.7956508399995702	0.0
7	0.0	0.0	0.0	0.9243994417909486	0.0
8	0.0	0.0	0.0	1.2000420208695695	0.0
9	0.0	0.0	0.0	1.309511161196592	0.0
10	0.0	0.0	0.0	1.5803786414605117	0.0
11	0.0	0.0	0.0	1.8055245505736683	0.0
12	0.0	0.0	0.0	2.0237465664055088	0.0
13	0.0	0.0	0.0	2.113100602975603	0.0
14	0.0	0.0	0.0	2.145153453769284	0.0
15	0.0	0.0	0.0	2.2091994666214623	0.0
16	0.0	0.0	0.0	2.3362170950925116	0.0
17	0.0	0.0	0.0	2.501316136610802	0.0
18	0.0	0.0	0.0	2.742578004223575	0.0
19	0.0	0.0	0.0	2.842138814510093	0.0
20	0.0	0.0	0.0	2.947489432109436	0.0
21	0.0	2.3875494073505484E-4	0.0	3.0823859736247416	0.0
22	5.968873518376371E-5	2.3875494073505484E-4	0.0	3.217461581345599	0.0
23	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.3772483254325345	0.0
24	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.4934026041001385	0.0
25	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.584189170314643	0.0
26	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.67945239166793	0.0
27	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.772626507289785	0.0
28	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.8712919865485467	0.0
29	1.1937747036752742E-4	2.3875494073505484E-4	0.0	3.9807014381403856	0.0
30	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.129386077483141	0.0
31	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.261596625915177	0.0
32	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.377333083436495	0.0
33	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.48763786605609	0.0
34	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.61053697179946	0.0
35	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.766443948099451	0.0
36	1.1937747036752742E-4	2.3875494073505484E-4	0.0	4.898594807796304	0.0
37	1.1937747036752742E-4	2.3875494073505484E-4	0.0	5.035043256426388	0.0
38	1.1937747036752742E-4	2.3875494073505484E-4	0.0	5.163015904660377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	25	4.4437154E-5	44.0	11
AATCCGC	30	2.5284135E-6	44.0	23
CTCCGTC	20	7.857515E-4	44.0	15
GTCGACA	30	2.5284135E-6	44.0	13
CGACGTA	20	7.857515E-4	44.0	39
CGCATCA	25	4.4437154E-5	44.0	19
TCTTACG	45	4.802132E-10	44.0	1
TTACTCG	30	2.5284135E-6	44.0	28
CGTCTAC	25	4.4437154E-5	44.0	35
GCGCATC	20	7.857515E-4	44.0	18
TCTAGCG	60	0.0	44.0	1
GCGCACC	20	7.857515E-4	44.0	21
ATCCGCG	20	7.857515E-4	44.0	10
GTATGCG	45	4.802132E-10	44.0	1
TCTCGCA	20	7.857515E-4	44.0	38
AGCGCAT	25	4.4437154E-5	44.0	34
GCGATCG	20	7.857515E-4	44.0	9
CCGACGG	30	2.5284135E-6	44.0	2
CGTAACG	20	7.857515E-4	44.0	11
TTCGGAC	25	4.4437154E-5	44.0	33
>>END_MODULE