FastQCFastQC Report
Thu 26 May 2016
SRR1545924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545924_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1255229
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116000.924134161973632No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20690.16483048113133142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG650.044.0000041
ACACGTA302.5279041E-644.019
CAAACGA254.443075E-544.035
AATCACG207.85676E-444.017
GTCGAAC254.443075E-544.017
ATTACGA207.85676E-444.018
CACGATT207.85676E-444.020
TCGATAT351.4462239E-744.016
TCGATAG600.044.01
TACGCAT207.85676E-444.034
CGAGGTA254.443075E-544.037
TACGACA207.85676E-444.01
TCGTCTA207.85676E-444.021
ACTACGC207.85676E-444.032
TATCGAA254.443075E-544.027
ATACCGG302.5279041E-644.02
ATCGCCA408.312782E-944.024
AATCGCG351.4462239E-744.01
CGTTTTT83200.042.9951931
TGCGTAG1300.042.3076931
Produced by FastQC (version 0.11.5)