##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545924_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1255229
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4873166569606	31.0	31.0	33.0	30.0	34.0
2	31.884041079356834	31.0	31.0	34.0	30.0	34.0
3	31.96008616754393	33.0	31.0	34.0	30.0	34.0
4	35.72171293046926	37.0	35.0	37.0	33.0	37.0
5	35.64851035149761	37.0	35.0	37.0	33.0	37.0
6	35.69799534586916	37.0	35.0	37.0	33.0	37.0
7	35.97267590216606	37.0	35.0	37.0	35.0	37.0
8	35.97075195044091	37.0	35.0	37.0	35.0	37.0
9	37.80447711134781	39.0	38.0	39.0	35.0	39.0
10	37.261954591552616	39.0	37.0	39.0	34.0	39.0
11	36.90285278622466	39.0	37.0	39.0	33.0	39.0
12	35.84341821293167	37.0	35.0	39.0	32.0	39.0
13	35.38368696070597	37.0	35.0	39.0	31.0	39.0
14	36.23566695798137	38.0	35.0	41.0	31.0	41.0
15	36.55608896862644	38.0	35.0	41.0	31.0	41.0
16	36.79768870859421	38.0	35.0	41.0	32.0	41.0
17	36.75711842221619	38.0	35.0	41.0	32.0	41.0
18	36.684833604067464	38.0	35.0	40.0	32.0	41.0
19	36.52794589672482	37.0	35.0	40.0	32.0	41.0
20	36.29541143488559	36.0	35.0	40.0	32.0	41.0
21	36.1414036801253	36.0	35.0	40.0	31.0	41.0
22	36.03543656177478	36.0	35.0	40.0	31.0	41.0
23	36.03237656236432	35.0	35.0	40.0	31.0	41.0
24	36.03778912055091	35.0	35.0	40.0	31.0	41.0
25	35.983250864981606	35.0	35.0	40.0	31.0	41.0
26	35.7535095189802	35.0	35.0	40.0	31.0	41.0
27	35.732210616548855	35.0	35.0	40.0	31.0	41.0
28	35.74188932856077	36.0	35.0	40.0	31.0	41.0
29	35.73723121438399	36.0	35.0	40.0	31.0	41.0
30	35.62302416531167	36.0	35.0	40.0	30.0	41.0
31	35.362759305274174	35.0	34.0	40.0	30.0	41.0
32	35.130652653818544	35.0	34.0	40.0	29.0	41.0
33	34.87048339386678	35.0	34.0	40.0	27.0	41.0
34	34.63739046819345	35.0	34.0	40.0	26.0	41.0
35	34.400087155411484	35.0	34.0	40.0	24.0	41.0
36	34.16944796527167	35.0	33.0	40.0	23.0	41.0
37	34.05802287869385	35.0	33.0	40.0	23.0	41.0
38	33.99686033385143	35.0	33.0	40.0	23.0	41.0
39	33.93145314520299	35.0	33.0	40.0	23.0	41.0
40	33.7753350185504	35.0	33.0	40.0	23.0	41.0
41	33.751894674198894	35.0	33.0	40.0	22.0	41.0
42	33.71488310101185	35.0	33.0	40.0	23.0	41.0
43	33.605145355947	35.0	33.0	40.0	22.0	41.0
44	33.49514630398119	35.0	33.0	40.0	22.0	41.0
45	33.39227742507543	35.0	33.0	39.0	22.0	41.0
46	33.38152321209915	35.0	33.0	39.0	22.0	41.0
47	33.321772361855885	35.0	33.0	39.0	22.0	41.0
48	33.243888565353416	35.0	33.0	39.0	22.0	41.0
49	33.21447321564432	35.0	33.0	39.0	21.0	41.0
50	33.0269146108001	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	14.0
14	16.0
15	32.0
16	113.0
17	268.0
18	680.0
19	1239.0
20	2182.0
21	3596.0
22	5443.0
23	7971.0
24	11854.0
25	17546.0
26	24401.0
27	30248.0
28	31301.0
29	31264.0
30	34074.0
31	40375.0
32	50101.0
33	66091.0
34	127568.0
35	228183.0
36	73610.0
37	90638.0
38	139678.0
39	236739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.91558751431013	22.42204410509955	28.608883319298712	16.053485061291607
2	33.78690262892269	23.771518981795353	29.985763553901318	12.455814835380636
3	28.0183137897547	23.470538045249114	34.999032049132076	13.512116115864117
4	24.636540424097912	27.144130672570505	33.98128946988956	14.238039433442026
5	21.585384021561005	31.54954195608929	33.540334074499555	13.324739947850153
6	19.028878395894296	40.850633629401486	29.941229847302765	10.179258127401454
7	86.6585300371486	3.345285999606446	8.946893355714376	1.0492906075305781
8	88.99627079998949	1.644082474193952	8.29984010885663	1.0598066169599332
9	86.30218071762205	2.660789385841149	9.417564444416119	1.6194654521206886
10	55.69182993700751	17.924458405597704	15.494144893083254	10.889566764311533
11	50.57626935005485	18.644486384556124	19.441074098829773	11.33817016655925
12	45.45576942533992	19.123203813806086	23.174177779512743	12.246848981341254
13	21.456323905837102	40.75726421234691	23.659188881072694	14.12722300074329
14	15.076053851528288	42.14920146045064	29.209490857843466	13.565253830177602
15	14.116149324147228	21.043172202044406	50.69616779089712	14.144510682911246
16	16.022176033217843	15.043788822597312	49.82588834387988	19.108146800304965
17	17.23789045664178	15.878696237897627	29.632202570208303	37.25121073525229
18	22.593407258755175	20.87850105438928	36.39885630430782	20.129235382547726
19	29.45024374038522	22.467374479079115	27.523981679836908	20.558400100698755
20	31.286960387307815	21.592952361680616	25.950483935600595	21.169603315410974
21	22.0459374345239	26.717276289824405	30.591310430208352	20.645475845443343
22	22.565683233895967	21.547223654010544	28.318179391967526	27.56891372012597
23	19.70644400344479	28.40597213735502	26.899553786599895	24.9880300726003
24	19.84466579405033	22.477651488294168	40.59952407090658	17.07815864674892
25	17.962539106410066	21.731652152714766	39.00379930674004	21.302009434135126
26	16.23265555528115	32.44937776294206	29.544728491773213	21.77323819000358
27	17.05577229334249	32.53278883773399	31.39379348310149	19.01764538582203
28	14.54690737706028	26.565590820479766	41.434670486421204	17.452831316038747
29	15.758558796841054	22.927609225089604	39.92211779683229	21.39171418123705
30	19.133958823449746	27.960157070940838	34.00925249496307	18.896631610646345
31	30.30443050630602	24.016494201456467	27.118398316163823	18.56067697607369
32	31.21772999189789	24.20108203363689	27.483271976667208	17.09791599779801
33	28.455524848453944	25.33338538226889	26.717196623086302	19.493893146190857
34	19.26190360484023	26.55523414452662	29.681197614140526	24.501664636492624
35	20.194721441266893	24.327353813527253	32.696902318222406	22.781022426983444
36	31.75842814339057	22.92450222230366	26.258475545099742	19.058594089206032
37	20.598552136701752	30.5131573601311	29.243827221965073	19.644463281202075
38	21.110729595954204	32.351786008768116	24.300984123215763	22.23650027206191
39	20.423046312664862	30.138723691055574	26.93500548505492	22.503224511224644
40	24.36288517872038	24.056964904411863	25.736499077060838	25.84365083980692
41	18.324704097818007	22.38213106931086	28.033052136303414	31.260112696567717
42	22.40180875362185	24.435222576916242	24.789341227775967	28.37362744168594
43	22.606472603803766	25.18695791763893	26.199442492166767	26.00712698639053
44	20.2563038298191	28.56299527815243	29.095408088882586	22.08529280314588
45	18.315781423150675	36.114366382548525	23.184375121989692	22.385477072311108
46	23.25137484873278	30.919617057923293	26.506478100808696	19.322529992535227
47	21.995986389734462	26.60908885948301	27.12015098440205	24.274773766380477
48	23.14892342353467	24.07895292412779	30.548768392062325	22.223355260275216
49	22.141935853935816	22.002837729211162	32.9195708512152	22.935655565637823
50	20.1275623810476	31.142843258082785	27.157913018262008	21.571681342607604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	299.0
1	871.0
2	1443.0
3	10289.5
4	19136.0
5	13696.5
6	8257.0
7	8571.0
8	8885.0
9	9399.5
10	9914.0
11	9835.0
12	9756.0
13	9081.5
14	8407.0
15	7709.0
16	7011.0
17	6229.0
18	5447.0
19	5140.5
20	4834.0
21	4402.0
22	3970.0
23	4028.0
24	4086.0
25	4040.0
26	3994.0
27	5057.5
28	6121.0
29	6451.0
30	6781.0
31	9630.5
32	12480.0
33	15550.0
34	18620.0
35	21211.0
36	23802.0
37	25428.0
38	27054.0
39	39211.0
40	51368.0
41	73632.5
42	95897.0
43	113724.5
44	131552.0
45	132067.0
46	132582.0
47	119455.0
48	106328.0
49	104649.5
50	102971.0
51	98522.5
52	94074.0
53	83781.0
54	73488.0
55	69131.5
56	64775.0
57	59304.0
58	53833.0
59	48570.0
60	43307.0
61	39918.0
62	36529.0
63	31010.5
64	25492.0
65	21952.0
66	18412.0
67	15467.5
68	12523.0
69	10518.0
70	8513.0
71	7053.0
72	5593.0
73	4519.5
74	3446.0
75	2696.5
76	1947.0
77	1585.5
78	1224.0
79	877.5
80	531.0
81	384.0
82	237.0
83	220.5
84	204.0
85	137.5
86	71.0
87	44.5
88	18.0
89	14.0
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1255229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.598875585196346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89215518356646	14.85904254492352
2	6.544660540104476	2.4344665426549414
3	2.32205533046032	1.2956285457952046
4	1.2693826899647176	0.9443636288262259
5	0.8818200178798136	0.8200430400541134
6	0.6766729983219836	0.7551214144591409
7	0.47158613981794095	0.6139680359523825
8	0.3727035840143298	0.5545494071391435
9	0.30772588504919046	0.5151019905336893
>10	4.0965208210207775	18.784463314617568
>50	1.971292620998255	26.646003279313202
>100	1.1908269653222754	30.482501543134454
>500	0.0017314823196252643	0.1942683632318868
>1k	4.328705799063161E-4	0.1665732463849042
>5k	0.0	0.0
>10k+	4.328705799063161E-4	0.9339051029796465
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11600	0.924134161973632	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2069	0.16483048113133142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02963602657363716	0.0
2	0.0	0.0	0.0	0.10914343119860997	0.0
3	0.0	0.0	0.0	0.15088880196362575	0.0
4	0.0	0.0	0.0	0.21852586261152349	0.0
5	0.0	0.0	0.0	0.3641566598604717	0.0
6	0.0	0.0	0.0	0.5085924560379023	0.0
7	0.0	0.0	0.0	0.5989345370446348	0.0
8	0.0	0.0	0.0	0.8178587333466643	0.0
9	0.0	0.0	0.0	0.9007121409718865	0.0
10	0.0	0.0	0.0	1.047458272554251	0.0
11	0.0	0.0	0.0	1.2026490783753403	0.0
12	0.0	0.0	0.0	1.3364095316472133	0.0
13	0.0	0.0	0.0	1.3883522448891796	0.0
14	7.966673810117517E-5	0.0	0.0	1.4109775985099133	0.0
15	7.966673810117517E-5	0.0	0.0	1.4459512965363293	0.0
16	7.966673810117517E-5	0.0	0.0	1.5255383678994032	0.0
17	7.966673810117517E-5	0.0	0.0	1.6204214529779029	0.0
18	7.966673810117517E-5	0.0	0.0	1.7710712547272252	0.0
19	7.966673810117517E-5	0.0	0.0	1.8304229746126006	0.0
20	7.966673810117517E-5	0.0	0.0	1.8914476959981008	0.0
21	7.966673810117517E-5	0.0	0.0	1.9876851156243203	0.0
22	7.966673810117517E-5	0.0	0.0	2.0776288629405473	0.0
23	7.966673810117517E-5	0.0	0.0	2.192667632758644	0.0
24	7.966673810117517E-5	0.0	0.0	2.2705817026215933	0.0
25	7.966673810117517E-5	0.0	0.0	2.3348727602692416	0.0
26	7.966673810117517E-5	0.0	0.0	2.4099188275605488	0.0
27	7.966673810117517E-5	0.0	0.0	2.4922942347571637	0.0
28	7.966673810117517E-5	0.0	0.0	2.5704473048344165	0.0
29	7.966673810117517E-5	0.0	0.0	2.6594350512934293	0.0
30	7.966673810117517E-5	0.0	0.0	2.7690564829206465	0.0
31	7.966673810117517E-5	0.0	0.0	2.868082238380407	0.0
32	7.966673810117517E-5	0.0	0.0	2.9515729799104387	0.0
33	7.966673810117517E-5	0.0	0.0	3.0447033967507124	0.0
34	7.966673810117517E-5	0.0	0.0	3.135921811876558	0.0
35	7.966673810117517E-5	0.0	0.0	3.266814262576789	0.0
36	7.966673810117517E-5	0.0	0.0	3.3675926862747754	0.0
37	7.966673810117517E-5	0.0	0.0	3.4682914432346608	0.0
38	7.966673810117517E-5	0.0	0.0	3.5936072222678095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	65	0.0	44.000004	1
ACACGTA	30	2.5279041E-6	44.0	19
CAAACGA	25	4.443075E-5	44.0	35
AATCACG	20	7.85676E-4	44.0	17
GTCGAAC	25	4.443075E-5	44.0	17
ATTACGA	20	7.85676E-4	44.0	18
CACGATT	20	7.85676E-4	44.0	20
TCGATAT	35	1.4462239E-7	44.0	16
TCGATAG	60	0.0	44.0	1
TACGCAT	20	7.85676E-4	44.0	34
CGAGGTA	25	4.443075E-5	44.0	37
TACGACA	20	7.85676E-4	44.0	1
TCGTCTA	20	7.85676E-4	44.0	21
ACTACGC	20	7.85676E-4	44.0	32
TATCGAA	25	4.443075E-5	44.0	27
ATACCGG	30	2.5279041E-6	44.0	2
ATCGCCA	40	8.312782E-9	44.0	24
AATCGCG	35	1.4462239E-7	44.0	1
CGTTTTT	8320	0.0	42.995193	1
TGCGTAG	130	0.0	42.307693	1
>>END_MODULE