FastQCFastQC Report
Thu 26 May 2016
SRR1545923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545923_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1383641
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129780.9379600633401294No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30370.21949335123778493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC302.5280915E-644.00000426
CGGAATA207.857038E-444.038
AACGTCC207.857038E-444.033
GGTACGC207.857038E-444.08
TTAATCG454.802132E-1044.020
GACGTGA207.857038E-444.037
CTATACG351.4463694E-744.01
CCTAAGT454.802132E-1044.020
TGCGATT254.4433124E-544.01
GAACGTA254.4433124E-544.011
CGTCGAA207.857038E-444.021
CGGTCTA2550.044.031
ACGTAAT207.857038E-444.010
TATCGTG408.314601E-944.01
TAATGCG650.043.9999961
CGTTTTT86350.042.9044571
CACGACG2800.040.85714326
GCGATAA700.040.8571439
CACGACC1900.040.52631827
AGTACGG1850.040.4324342
Produced by FastQC (version 0.11.5)