##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545923_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1383641
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.460972174140547	31.0	31.0	33.0	30.0	34.0
2	31.853156996648696	31.0	31.0	34.0	30.0	34.0
3	31.928724286140696	31.0	31.0	34.0	30.0	34.0
4	35.687426145943924	37.0	35.0	37.0	33.0	37.0
5	35.60709461486036	37.0	35.0	37.0	33.0	37.0
6	35.65537592482443	37.0	35.0	37.0	33.0	37.0
7	35.94204638341882	37.0	35.0	37.0	35.0	37.0
8	35.93635921456505	37.0	35.0	37.0	35.0	37.0
9	37.75312237784223	39.0	38.0	39.0	35.0	39.0
10	37.23985412401049	39.0	37.0	39.0	34.0	39.0
11	36.85631894400354	39.0	35.0	39.0	33.0	39.0
12	35.77735192871561	37.0	35.0	39.0	32.0	39.0
13	35.304959884825614	37.0	35.0	39.0	30.0	39.0
14	36.11895932543196	38.0	35.0	41.0	30.0	41.0
15	36.45799380041499	38.0	35.0	41.0	31.0	41.0
16	36.71916125642417	38.0	35.0	41.0	32.0	41.0
17	36.64991280252609	38.0	35.0	41.0	32.0	41.0
18	36.58594462002788	38.0	35.0	40.0	32.0	41.0
19	36.43204415018057	37.0	35.0	40.0	31.0	41.0
20	36.19302983938753	36.0	35.0	40.0	31.0	41.0
21	36.01249095683057	36.0	35.0	40.0	31.0	41.0
22	35.917080369835816	35.0	35.0	40.0	31.0	41.0
23	35.890396425084255	35.0	35.0	40.0	31.0	41.0
24	35.91466861707625	35.0	35.0	40.0	31.0	41.0
25	35.870516991040304	35.0	35.0	40.0	31.0	41.0
26	35.622512631527975	35.0	35.0	40.0	30.0	41.0
27	35.573398012923874	35.0	34.0	40.0	30.0	41.0
28	35.569576935057576	36.0	35.0	40.0	30.0	41.0
29	35.5251167029598	36.0	35.0	40.0	30.0	41.0
30	35.42320153854938	36.0	34.0	40.0	30.0	41.0
31	35.14310865318388	35.0	34.0	40.0	29.0	41.0
32	34.92895266908107	35.0	34.0	40.0	29.0	41.0
33	34.68906024033691	35.0	34.0	40.0	27.0	41.0
34	34.456463056529834	35.0	34.0	40.0	25.0	41.0
35	34.22464859020512	35.0	33.0	40.0	23.0	41.0
36	33.974843908210296	35.0	33.0	40.0	23.0	41.0
37	33.8507987259701	35.0	33.0	40.0	23.0	41.0
38	33.744031146807586	35.0	33.0	40.0	23.0	41.0
39	33.68629290401195	35.0	33.0	40.0	23.0	41.0
40	33.47025420611271	35.0	33.0	40.0	21.0	41.0
41	33.43607698817829	35.0	33.0	40.0	21.0	41.0
42	33.41613467655266	35.0	33.0	40.0	21.0	41.0
43	33.29413843619841	35.0	33.0	39.0	21.0	41.0
44	33.135483842991064	35.0	33.0	39.0	21.0	41.0
45	33.022461751277966	35.0	33.0	39.0	20.0	41.0
46	33.03624928720672	35.0	33.0	39.0	20.0	41.0
47	32.967628886394664	35.0	32.0	39.0	20.0	41.0
48	32.870859565450864	35.0	32.0	38.0	20.0	41.0
49	32.831655754635776	35.0	32.0	38.0	20.0	41.0
50	32.62597017578982	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	0.0
12	2.0
13	6.0
14	15.0
15	46.0
16	142.0
17	397.0
18	883.0
19	1733.0
20	2980.0
21	4535.0
22	6934.0
23	9787.0
24	13872.0
25	20138.0
26	28272.0
27	34146.0
28	36124.0
29	36697.0
30	40319.0
31	46282.0
32	57593.0
33	75375.0
34	142980.0
35	243415.0
36	85051.0
37	106614.0
38	154788.0
39	234509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.50919783383117	22.713333877790554	27.654066336571407	16.123401951806862
2	33.641818940028514	24.493781262625202	29.26091377748997	12.603486019856305
3	28.0169494832836	24.27081880343239	33.87757373480549	13.834657978478521
4	25.051874004889996	27.06258343023949	33.159034749620744	14.726507815249764
5	22.534602545024324	31.65886237831923	32.07754034464142	13.728994732015023
6	19.561287935237537	40.28017383121778	29.31829860491269	10.840239628631993
7	86.20892269020649	3.6394556102341573	8.812040117342576	1.3395815822167745
8	88.15090041419703	2.018225825918717	8.382015277084157	1.4488584828000906
9	85.7344499042743	2.9942738036817356	9.429685879501982	1.8415904125419817
10	56.93919159666416	18.533709249725906	14.239531786063003	10.287567367546929
11	50.83406750739534	18.58704678453443	18.494754058314257	12.084131649755971
12	43.260571203079415	19.831155624905595	23.71691789994659	13.191355272068407
13	21.797633923828506	39.4109454692366	24.905231920707756	13.886188686227136
14	15.241453527323923	40.95231349750405	29.95451854924796	13.851714425924067
15	14.666665703025568	19.712627769775544	51.39924301173499	14.221463515463912
16	17.963546902700916	14.620049564879908	47.76260605171428	19.653797480704892
17	17.659783137388963	15.644737327095685	29.426997320836833	37.268482214678514
18	24.321699053439442	20.318565292586733	34.73834614614629	20.621389507827537
19	30.599338990388404	22.11643049027891	26.508971619083272	20.775258900249412
20	32.08368355664511	22.0070090435308	24.98169684188312	20.927610557940966
21	23.37737895884843	25.374862410119388	30.57693433484553	20.670824296186655
22	23.68779184774085	20.963313460644777	27.798684774446553	27.550209917167823
23	20.06965679681362	27.759729583034904	26.505863876540232	25.664749743611242
24	20.974154423004233	22.89257112213356	38.88747153343967	17.245802921422538
25	19.008760220317264	21.611530736657848	37.19042728569043	22.189281757334452
26	17.0273213933383	31.206071517105954	28.590075026686833	23.176532062868908
27	18.98194690674821	29.8082378304777	31.62980859919589	19.580006663578196
28	16.065944851301747	25.090323284724867	40.23269041608336	18.611041447890024
29	16.471685935874987	23.337195125036043	38.561086293337645	21.630032645751317
30	20.897544955664078	26.402730187960604	34.28360391170831	18.416120944667007
31	31.264468167682224	23.091322098723584	26.256666288437536	19.387543445156656
32	31.25608449012425	24.272119718915526	27.017557299906553	17.454238491053676
33	29.249855995883323	24.61801869126457	25.781832137093364	20.35029317575874
34	19.843875687407355	26.940080555577637	29.77318538551546	23.442858371499543
35	20.40550981070957	24.77940448425567	31.984958526091667	22.830127178943094
36	32.514214308480305	22.647637645892253	26.55385320325142	18.28429484237602
37	21.436846696505814	29.712042357808134	28.783911433673907	20.06719951201215
38	22.835186294710837	30.537328685692316	22.979877005668378	23.64760801392847
39	22.006575405036422	28.039715504238455	26.906473572263323	23.047235518461797
40	25.507628062481523	23.501255022075814	25.128411199147756	25.862705716294904
41	18.882065506876422	22.06952525980366	27.74411859723729	31.304290636082627
42	25.070448187065864	23.6848286513626	24.17144331513738	27.073279846434158
43	24.192836147526705	24.274432457552212	25.213115251716307	26.319616143204776
44	21.267294045203922	28.677164090974465	28.012902190669404	22.042639673152213
45	18.929838014340426	34.29885353209395	23.254731538021783	23.516576915543844
46	24.07987331974118	28.969219616938208	26.307907903856563	20.64299915946405
47	23.071157908734996	26.120937439697144	27.06424571113461	23.74365894043325
48	25.310756186033807	22.96231464664606	29.930307066645177	21.79662210067496
49	23.189902583112236	21.641234973522756	31.43524946138485	23.73361298198015
50	21.224869745837253	30.365896934248116	26.86657883078053	21.5426544891341
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	488.0
1	1285.5
2	2083.0
3	11814.5
4	21546.0
5	15306.5
6	9067.0
7	9271.5
8	9476.0
9	9862.5
10	10249.0
11	10026.5
12	9804.0
13	9126.5
14	8449.0
15	7746.5
16	7044.0
17	6366.0
18	5688.0
19	5220.5
20	4753.0
21	4640.0
22	4527.0
23	4578.0
24	4629.0
25	5026.5
26	5424.0
27	5367.0
28	5310.0
29	6830.0
30	8350.0
31	11158.5
32	13967.0
33	14966.0
34	15965.0
35	17814.0
36	19663.0
37	24064.5
38	28466.0
39	38274.5
40	48083.0
41	71244.0
42	94405.0
43	111352.0
44	128299.0
45	133383.0
46	138467.0
47	129390.5
48	120314.0
49	117120.0
50	113926.0
51	107506.0
52	101086.0
53	90943.5
54	80801.0
55	77220.0
56	73639.0
57	67761.0
58	61883.0
59	59472.5
60	57062.0
61	51989.5
62	46917.0
63	41317.5
64	35718.0
65	31592.0
66	27466.0
67	24040.5
68	20615.0
69	17326.0
70	14037.0
71	12225.5
72	10414.0
73	8601.5
74	6789.0
75	5239.0
76	3689.0
77	3124.0
78	2559.0
79	2001.0
80	1443.0
81	1042.5
82	642.0
83	408.0
84	174.0
85	140.5
86	107.0
87	111.5
88	116.0
89	71.5
90	27.0
91	18.5
92	10.0
93	7.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1383641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.360410274366238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97321877804055	15.483143265045152
2	6.82192576033401	2.6415056316266856
3	2.3070277964714925	1.3399501396216558
4	1.2758564406175323	0.9880441656619205
5	0.8567796996483206	0.8293803249969884
6	0.6138700213789914	0.7130865281418748
7	0.45720552724084207	0.6196180610963441
8	0.380548618872146	0.5894061912566542
9	0.2908923987229578	0.5068616566473894
>10	4.086613235844084	19.779913947966264
>50	1.8667879593853767	26.223390178309025
>100	1.0654940753438236	28.680870204096976
>500	0.0030237504798689557	0.4329110636967655
>1k	3.7796880998361947E-4	0.22223646052181825
>5k	0.0	0.0
>10k+	3.7796880998361947E-4	0.949682181314507
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12978	0.9379600633401294	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3037	0.21949335123778493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028331048299378235	0.0
2	0.0	0.0	0.0	0.11874467437724091	0.0
3	0.0	0.0	0.0	0.17005856287866578	0.0
4	0.0	0.0	0.0	0.25295578838730565	0.0
5	0.0	0.0	0.0	0.43616805226211136	0.0
6	0.0	0.0	0.0	0.629064909178031	0.0
7	0.0	0.0	0.0	0.7446295679298315	0.0
8	0.0	0.0	0.0	1.0510674372904532	0.0
9	0.0	0.0	0.0	1.1777621507312952	0.0
10	0.0	0.0	0.0	1.3960268595683418	0.0
11	0.0	0.0	0.0	1.5860327931884066	0.0
12	0.0	0.0	0.0	1.749225413239417	0.0
13	0.0	0.0	0.0	1.8305326309353365	0.0
14	0.0	0.0	0.0	1.8631277910961008	0.0
15	0.0	0.0	0.0	1.9038898095676553	0.0
16	0.0	0.0	0.0	1.9917738777616447	0.0
17	0.0	0.0	0.0	2.092811646951774	0.0
18	0.0	0.0	0.0	2.255064716931632	0.0
19	0.0	0.0	0.0	2.318014571698873	0.0
20	0.0	0.0	0.0	2.3892035578593003	0.0
21	0.0	0.0	0.0	2.487784042247953	0.0
22	0.0	0.0	0.0	2.580582680044896	0.0
23	0.0	0.0	0.0	2.6907268576169687	0.0
24	0.0	0.0	0.0	2.7753586371031216	0.0
25	0.0	0.0	0.0	2.8437289730500903	0.0
26	0.0	0.0	0.0	2.916580240105634	0.0
27	0.0	0.0	0.0	2.996947907730401	0.0
28	0.0	0.0	0.0	3.077315575355168	0.0
29	0.0	0.0	0.0	3.180376990852396	0.0
30	0.0	0.0	0.0	3.288063883622992	0.0
31	0.0	0.0	0.0	3.3937271300864893	0.0
32	0.0	0.0	0.0	3.481828017527668	0.0
33	0.0	0.0	0.0	3.5810589596578883	0.0
34	0.0	0.0	0.0	3.6872281176981603	0.0
35	0.0	0.0	0.0	3.816813754434857	0.0
36	0.0	0.0	0.0	3.9162615158122662	0.0
37	0.0	0.0	0.0	4.028501612773834	0.0
38	0.0	0.0	0.0	4.164808646173393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	30	2.5280915E-6	44.000004	26
CGGAATA	20	7.857038E-4	44.0	38
AACGTCC	20	7.857038E-4	44.0	33
GGTACGC	20	7.857038E-4	44.0	8
TTAATCG	45	4.802132E-10	44.0	20
GACGTGA	20	7.857038E-4	44.0	37
CTATACG	35	1.4463694E-7	44.0	1
CCTAAGT	45	4.802132E-10	44.0	20
TGCGATT	25	4.4433124E-5	44.0	1
GAACGTA	25	4.4433124E-5	44.0	11
CGTCGAA	20	7.857038E-4	44.0	21
CGGTCTA	255	0.0	44.0	31
ACGTAAT	20	7.857038E-4	44.0	10
TATCGTG	40	8.314601E-9	44.0	1
TAATGCG	65	0.0	43.999996	1
CGTTTTT	8635	0.0	42.904457	1
CACGACG	280	0.0	40.857143	26
GCGATAA	70	0.0	40.857143	9
CACGACC	190	0.0	40.526318	27
AGTACGG	185	0.0	40.432434	2
>>END_MODULE