FastQCFastQC Report
Thu 26 May 2016
SRR1545922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545922_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1238197
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100620.8126332078013434No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13130.10604128422213913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.8567176E-444.026
GTACACG254.44304E-544.027
GTTTACG408.312782E-944.01
CTACGAT254.44304E-544.035
GCGTAAG502.7284841E-1144.01
CGTTTTT61050.042.6666641
GCGATAT850.041.4117669
TAGACGG800.041.252
CGTAAGG1400.040.8571432
CGGTCTA2350.040.2553231
TCGTTAG557.8216544E-1140.01
TACGGGA2600.039.769234
CGTTAGG1700.038.8235322
GCAATCC1700.038.82353235
TCACGAC2500.038.7225
AACGTAG404.124886E-738.51
TCTAACG404.124886E-738.51
TAATGCG404.124886E-738.51
ATTGGGA6500.038.2461554
TATGGGC4100.038.097564
Produced by FastQC (version 0.11.5)