##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545922_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1238197
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499364802208373	31.0	31.0	33.0	30.0	34.0
2	31.89833685592842	31.0	31.0	34.0	30.0	34.0
3	32.005702646670926	33.0	31.0	34.0	30.0	34.0
4	35.76701122680801	37.0	35.0	37.0	35.0	37.0
5	35.70081739820077	37.0	35.0	37.0	33.0	37.0
6	35.75619550039291	37.0	35.0	37.0	33.0	37.0
7	35.981624894907675	37.0	35.0	37.0	35.0	37.0
8	35.96505321851046	37.0	35.0	37.0	35.0	37.0
9	37.776290848709856	39.0	38.0	39.0	35.0	39.0
10	37.26816007468925	39.0	37.0	39.0	34.0	39.0
11	36.94312778984281	39.0	37.0	39.0	33.0	39.0
12	36.234382735542084	38.0	35.0	39.0	32.0	39.0
13	35.96479316296195	38.0	35.0	39.0	32.0	39.0
14	36.90826015569412	39.0	35.0	41.0	31.0	41.0
15	37.08272350845625	39.0	35.0	41.0	32.0	41.0
16	37.23468640289065	39.0	35.0	41.0	32.0	41.0
17	37.15575954391748	39.0	35.0	41.0	32.0	41.0
18	37.07590472275413	38.0	35.0	41.0	32.0	41.0
19	36.97255444812094	38.0	35.0	41.0	32.0	41.0
20	36.79214939141348	38.0	35.0	41.0	32.0	41.0
21	36.652701468344695	38.0	35.0	40.0	32.0	41.0
22	36.55807839947924	38.0	35.0	40.0	31.0	41.0
23	36.490789430114916	38.0	35.0	40.0	31.0	41.0
24	36.55915011908444	38.0	35.0	40.0	31.0	41.0
25	36.51614403846884	38.0	35.0	40.0	31.0	41.0
26	36.277879852721334	38.0	35.0	40.0	31.0	41.0
27	36.26042544118585	38.0	35.0	40.0	31.0	41.0
28	36.221597209490895	38.0	35.0	40.0	31.0	41.0
29	36.15596791140667	38.0	35.0	40.0	31.0	41.0
30	36.0409014074497	37.0	35.0	40.0	30.0	41.0
31	35.83773906736973	37.0	35.0	40.0	30.0	41.0
32	35.664800512357886	37.0	35.0	40.0	30.0	41.0
33	35.45510932428362	37.0	34.0	40.0	29.0	41.0
34	35.211112609705886	37.0	34.0	40.0	27.0	41.0
35	35.00435875712831	37.0	34.0	40.0	26.0	41.0
36	34.79637327501197	37.0	34.0	40.0	25.0	41.0
37	34.70487248798051	37.0	34.0	40.0	24.0	41.0
38	34.58509510199104	36.0	34.0	40.0	24.0	41.0
39	34.46184492451524	36.0	34.0	40.0	23.0	41.0
40	34.278751281096625	36.0	33.0	40.0	23.0	41.0
41	34.21275693609337	36.0	33.0	40.0	23.0	41.0
42	34.191282162693014	36.0	33.0	40.0	23.0	41.0
43	34.09816693143337	35.0	33.0	40.0	23.0	41.0
44	33.97057899510337	35.0	33.0	40.0	23.0	41.0
45	33.85989224654881	35.0	33.0	40.0	23.0	41.0
46	33.807665500724035	35.0	33.0	40.0	23.0	41.0
47	33.728510083613514	35.0	33.0	40.0	23.0	41.0
48	33.58456287650511	35.0	33.0	39.0	23.0	41.0
49	33.5261884821236	35.0	33.0	39.0	23.0	41.0
50	33.374152901355764	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	5.0
13	9.0
14	9.0
15	36.0
16	99.0
17	231.0
18	526.0
19	1076.0
20	1871.0
21	3055.0
22	4731.0
23	6783.0
24	10002.0
25	14722.0
26	20853.0
27	25431.0
28	27312.0
29	28905.0
30	32622.0
31	38168.0
32	46908.0
33	62035.0
34	112640.0
35	172348.0
36	87361.0
37	114829.0
38	170350.0
39	255273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.036912542995985	21.68192945064477	28.536896794290406	14.744261212068837
2	33.931757224415826	23.78466431432155	29.364955657298474	12.91862280396415
3	29.485534208207582	24.36494354290957	33.2128086241527	12.936713624730151
4	25.992552073700715	27.41776954717222	32.054996095128644	14.534682283998427
5	22.596162000069455	31.45105342687795	31.284924773683027	14.667859799369568
6	20.373090873261688	41.34043290364942	28.125734434827415	10.16074178826148
7	87.6968689150434	3.140130366977145	7.8493163850340455	1.3136843329454038
8	89.22207047828415	1.9352332464058628	7.577711785765915	1.264984489544071
9	86.15236509214608	3.1945643544605584	8.647735376519245	2.0053351768741163
10	51.33876111798041	23.148416608988715	14.286579599207558	11.22624267382331
11	42.44938406408673	21.053354191618944	21.147523374713394	15.349738369580932
12	37.60936264584715	20.131287670701838	26.82594126782733	15.433408415623685
13	23.72619219720287	31.193420756147848	27.870605404471178	17.2097816421781
14	18.796362775874922	33.16556250742006	31.006859166998467	17.03121554970655
15	18.39190371160647	21.591233059036647	42.43242391961861	17.584439309738272
16	21.44997928439497	17.499961637768465	40.43209602349223	20.61796305434434
17	21.694205364735982	18.679095491266736	28.9676037011881	30.659095442809182
18	25.079046387610372	21.941742711377916	32.33839203293176	20.640818868079958
19	28.66014051075879	24.050454006914894	26.396526562412927	20.89287891991339
20	30.56209956897004	23.17482597680337	25.642930809879203	20.620143644347387
21	25.6820199047486	25.26956534380232	29.13421692993926	19.914197821509823
22	25.056029048689343	21.259621853388435	28.028900086173685	25.655449011748534
23	22.699457356139614	25.665867386207523	27.20827138169451	24.42640387595835
24	22.904190528647703	22.86978566415522	34.3245864753347	19.901437331862375
25	22.13508835831455	22.31139309819035	33.0380383735383	22.5154801699568
26	20.682492365915923	27.18129667573092	28.058459195103847	24.077751763249307
27	20.691376251113514	26.875367974563012	30.26675076744654	22.166505006876935
28	19.17344332121625	24.74218561343631	35.56752277707021	20.516848288277227
29	20.441173738912305	23.789752357661985	33.982556895227496	21.78651700819821
30	23.23176360466065	24.94118464186232	31.775880574738913	20.051171178738116
31	30.026562816740793	23.150354911213643	26.343304013820095	20.479778258225465
32	30.21433584478076	23.381255163758272	27.044404081095337	19.360004910365635
33	28.25463153278517	24.084858871407377	26.605136339370876	21.055373256436578
34	22.371399704570436	25.126938605084653	29.150773261443856	23.350888428901058
35	22.88399988047136	25.05942107758297	29.62808018433254	22.42849885761313
36	30.723463229195353	23.95507338492986	25.64559597543848	19.675867410436304
37	23.67862303009941	28.702137058965576	26.618462167167262	21.000777743767753
38	23.54907983140001	29.770060822308565	24.673375884451342	22.00748346184008
39	23.549160593992717	27.070894211502694	26.123791286846927	23.256153907657666
40	25.831672989031635	24.00441932907284	26.066853658989643	24.097054022905887
41	21.650997377638614	23.10650082337463	27.380941804898573	27.861559994088182
42	23.32068321922925	24.371808363289524	25.538747065289286	26.768761352191937
43	22.97873440171475	24.009345847227863	26.894831759405008	26.117087991652376
44	22.19719479210497	26.016780851512323	28.721197030844042	23.064827325538666
45	21.761399841866844	29.984243218163183	25.19986722629759	23.05448971367238
46	24.156818341507854	27.396852035661528	27.108448817110688	21.33788080571993
47	23.171999286058682	25.71594019368485	28.58220460879812	22.529855911458355
48	23.315191362925287	24.756319067159748	29.912768323618938	22.015721246296025
49	23.212703632782183	23.24549324542056	30.684777947289486	22.857025174507772
50	22.310181659299772	27.575418128133084	28.170961486742417	21.943438725824727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	1179.0
2	2013.0
3	10229.5
4	18446.0
5	12897.0
6	7348.0
7	7197.0
8	7046.0
9	7263.5
10	7481.0
11	7246.5
12	7012.0
13	6520.5
14	6029.0
15	5679.5
16	5330.0
17	4829.5
18	4329.0
19	4161.5
20	3994.0
21	3874.5
22	3755.0
23	3858.0
24	3961.0
25	4220.5
26	4480.0
27	5816.0
28	7152.0
29	8978.0
30	10804.0
31	13259.0
32	15714.0
33	17898.5
34	20083.0
35	22420.5
36	24758.0
37	26664.0
38	28570.0
39	34548.0
40	40526.0
41	57241.0
42	73956.0
43	82009.0
44	90062.0
45	93718.5
46	97375.0
47	95662.0
48	93949.0
49	95486.5
50	97024.0
51	91385.0
52	85746.0
53	80848.5
54	75951.0
55	73636.5
56	71322.0
57	70409.0
58	69496.0
59	65894.5
60	62293.0
61	58634.0
62	54975.0
63	49464.0
64	43953.0
65	37753.0
66	31553.0
67	27222.0
68	22891.0
69	19066.5
70	15242.0
71	12553.0
72	9864.0
73	7840.0
74	5816.0
75	4750.0
76	3684.0
77	2815.5
78	1947.0
79	1373.5
80	800.0
81	716.5
82	633.0
83	428.5
84	224.0
85	171.0
86	118.0
87	95.5
88	73.0
89	57.5
90	42.0
91	33.0
92	24.0
93	14.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1238197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.791197624320493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0029396467424	16.561950778714667
2	6.3543536264465015	2.7693795129742664
3	2.5883041404601066	1.692067411098395
4	1.5060712036378934	1.3127638093908627
5	1.0368955382169984	1.1297597794531387
6	0.7979072412005647	1.0432412627326721
7	0.6692993300184673	1.0209383779180405
8	0.5727301980355277	0.9984381544646717
9	0.49776478600130536	0.976220173996384
>10	8.263054858088243	44.57559353644341
>50	1.5887560139476127	22.634217955494158
>100	0.11779677846814673	3.8117318265653957
>500	0.0033763407841187905	0.5437973729880256
>1k	3.75148976013199E-4	0.10733703408497858
>5k	0.0	0.0
>10k+	3.75148976013199E-4	0.8225630136809251
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10062	0.8126332078013434	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1313	0.10604128422213913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032870375231081966	0.0
2	0.0	0.0	0.0	0.1116946657115144	0.0
3	0.0	0.0	0.0	0.1563563794775791	0.0
4	0.0	0.0	0.0	0.2103057914047603	0.0
5	0.0	0.0	0.0	0.3496212638215082	0.0
6	0.0	0.0	0.0	0.5024240892200514	0.0
7	0.0	0.0	0.0	0.6024081789892884	0.0
8	0.0	0.0	0.0	0.856164245269533	0.0
9	0.0	0.0	0.0	0.984092192114825	0.0
10	0.0	0.0	0.0	1.1901983286989066	0.0
11	0.0	0.0	0.0	1.3702181478391564	0.0
12	0.0	0.0	0.0	1.5197904695294853	0.0
13	0.0	0.0	0.0	1.579231737760631	0.0
14	0.0	0.0	0.0	1.6057218681679895	0.0
15	0.0	0.0	0.0	1.6419842722926965	0.0
16	0.0	0.0	0.0	1.7216161887001826	0.0
17	0.0	0.0	0.0	1.807789875116803	0.0
18	0.0	0.0	0.0	1.939513663819247	0.0
19	0.0	0.0	0.0	1.9993587450139194	0.0
20	0.0	0.0	0.0	2.067926186220771	0.0
21	0.0	0.0	0.0	2.1617723189444007	0.0
22	0.0	0.0	0.0	2.2587681927835392	0.0
23	0.0	0.0	0.0	2.3674746425649555	0.0
24	0.0	0.0	0.0	2.4541329045378077	0.0
25	0.0	0.0	0.0	2.5248809357477042	0.0
26	0.0	0.0	0.0	2.596194305106538	0.0
27	0.0	0.0	0.0	2.6826910418939796	0.0
28	0.0	0.0	0.0	2.763049821635814	0.0
29	0.0	0.0	0.0	2.8540692636147558	0.0
30	0.0	0.0	0.0	2.9749708648946815	0.0
31	0.0	0.0	0.0	3.0756010554055613	0.0
32	0.0	0.0	0.0	3.165651346272039	0.0
33	0.0	0.0	0.0	3.260385867515428	0.0
34	0.0	0.0	0.0	3.3612583458044236	0.0
35	0.0	0.0	0.0	3.485228925607153	0.0
36	0.0	0.0	0.0	3.5897357205678904	0.0
37	0.0	0.0	0.0	3.6928695514526364	0.0
38	0.0	0.0	0.0	3.833719513130786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.8567176E-4	44.0	26
GTACACG	25	4.44304E-5	44.0	27
GTTTACG	40	8.312782E-9	44.0	1
CTACGAT	25	4.44304E-5	44.0	35
GCGTAAG	50	2.7284841E-11	44.0	1
CGTTTTT	6105	0.0	42.666664	1
GCGATAT	85	0.0	41.411766	9
TAGACGG	80	0.0	41.25	2
CGTAAGG	140	0.0	40.857143	2
CGGTCTA	235	0.0	40.25532	31
TCGTTAG	55	7.8216544E-11	40.0	1
TACGGGA	260	0.0	39.76923	4
CGTTAGG	170	0.0	38.823532	2
GCAATCC	170	0.0	38.823532	35
TCACGAC	250	0.0	38.72	25
AACGTAG	40	4.124886E-7	38.5	1
TCTAACG	40	4.124886E-7	38.5	1
TAATGCG	40	4.124886E-7	38.5	1
ATTGGGA	650	0.0	38.246155	4
TATGGGC	410	0.0	38.09756	4
>>END_MODULE