FastQCFastQC Report
Thu 26 May 2016
SRR1545921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545921_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1666395
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128830.7731060162806538No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36890.2213760843017412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA207.8575034E-444.01
GTACACG408.3164196E-944.01
CAACGTA207.8575034E-444.026
CTATACG454.802132E-1044.01
ATTACGT207.8575034E-444.035
ATTACGC207.8575034E-444.027
TCCAACG302.5284062E-644.037
TTACGTT207.8575034E-444.036
CGTAAGA254.443706E-544.038
TCGAGGT302.5284062E-644.044
CACGTAC254.443706E-544.037
CAATCGA207.8575034E-444.026
CAATACG254.443706E-544.01
ATACCCG207.8575034E-444.01
ATCGATA408.3164196E-944.015
TAATCGG454.802132E-1044.02
CGTTTTT104550.043.2214241
CGACGGT2200.043.028
AATGCGG2550.042.274512
CGGTCTA2200.042.031
Produced by FastQC (version 0.11.5)