##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545921_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1666395
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539116475985587	31.0	31.0	33.0	30.0	34.0
2	31.94150366509741	31.0	31.0	34.0	30.0	34.0
3	31.991489412774282	33.0	31.0	34.0	30.0	34.0
4	35.73733718596131	37.0	35.0	37.0	35.0	37.0
5	35.66984658499335	37.0	35.0	37.0	33.0	37.0
6	35.72213610818564	37.0	35.0	37.0	33.0	37.0
7	36.014078894859864	37.0	35.0	37.0	35.0	37.0
8	36.020298308624305	37.0	35.0	37.0	35.0	37.0
9	37.837244470848745	39.0	38.0	39.0	35.0	39.0
10	37.35158050762274	39.0	37.0	39.0	34.0	39.0
11	36.94210316281554	39.0	37.0	39.0	33.0	39.0
12	35.84329825761599	37.0	35.0	39.0	32.0	39.0
13	35.37995733304528	37.0	35.0	39.0	31.0	39.0
14	36.265673504781276	38.0	35.0	41.0	31.0	41.0
15	36.59282403031694	38.0	35.0	41.0	32.0	41.0
16	36.82876448861165	38.0	35.0	41.0	32.0	41.0
17	36.79580231577747	38.0	35.0	41.0	32.0	41.0
18	36.69511010294678	38.0	35.0	40.0	32.0	41.0
19	36.54663930220626	37.0	35.0	40.0	32.0	41.0
20	36.31172681147027	36.0	35.0	40.0	32.0	41.0
21	36.145026839374815	36.0	35.0	40.0	31.0	41.0
22	36.047933413146346	35.0	35.0	40.0	31.0	41.0
23	36.032336270812145	35.0	35.0	40.0	31.0	41.0
24	36.057557781918455	35.0	35.0	40.0	31.0	41.0
25	36.018421202656036	35.0	35.0	40.0	31.0	41.0
26	35.76968605882759	35.0	35.0	40.0	31.0	41.0
27	35.723150873592395	35.0	35.0	40.0	31.0	41.0
28	35.760379141800115	36.0	35.0	40.0	31.0	41.0
29	35.76019191128154	36.0	35.0	40.0	31.0	41.0
30	35.66497379072789	36.0	35.0	40.0	30.0	41.0
31	35.38349731006154	35.0	34.0	40.0	30.0	41.0
32	35.097531497634115	35.0	34.0	40.0	29.0	41.0
33	34.84466828092979	35.0	34.0	40.0	27.0	41.0
34	34.61778509897113	35.0	34.0	40.0	26.0	41.0
35	34.35131226389902	35.0	34.0	40.0	24.0	41.0
36	34.12071807704656	35.0	33.0	40.0	23.0	41.0
37	33.99895522970244	35.0	33.0	40.0	23.0	41.0
38	33.95820198692387	35.0	33.0	40.0	23.0	41.0
39	33.909348623825686	35.0	33.0	40.0	23.0	41.0
40	33.724067222957345	35.0	33.0	40.0	23.0	41.0
41	33.69831702567519	35.0	33.0	40.0	22.0	41.0
42	33.669452920826096	35.0	33.0	40.0	22.0	41.0
43	33.54474419330351	35.0	33.0	40.0	22.0	41.0
44	33.43725707290288	35.0	33.0	40.0	22.0	41.0
45	33.299245977094266	35.0	33.0	39.0	22.0	41.0
46	33.29198059283663	35.0	33.0	39.0	22.0	41.0
47	33.23413416386871	35.0	33.0	39.0	22.0	41.0
48	33.15733784606891	35.0	33.0	39.0	21.0	41.0
49	33.14050030154915	35.0	33.0	39.0	20.0	41.0
50	32.93250279795607	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	3.0
13	2.0
14	19.0
15	44.0
16	115.0
17	305.0
18	707.0
19	1539.0
20	2636.0
21	4503.0
22	6904.0
23	10483.0
24	15484.0
25	23127.0
26	33147.0
27	40318.0
28	40915.0
29	41641.0
30	45468.0
31	53639.0
32	65696.0
33	88120.0
34	172497.0
35	309081.0
36	96187.0
37	121692.0
38	182481.0
39	309637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.928924414679592	22.849924537699646	28.800734519726717	16.420416527894048
2	33.92208930055599	24.31140275864966	29.530153415006644	12.236354525787704
3	27.767246061107958	24.199604535539294	35.09522052094491	12.937928882407832
4	24.675122044893318	26.96185478232952	34.111120112578355	14.251903060198812
5	22.03763213403785	31.10973088613444	32.96205281460878	13.89058416521893
6	19.916406374239003	39.74321814455756	29.94290069281293	10.397474788390507
7	86.48327677411419	3.424518196466024	8.90935222441258	1.1828528050072162
8	88.63654775728443	1.796452821809955	8.434074754184932	1.1329246667206754
9	85.72505318366895	3.081142226182868	9.569099763261411	1.6247048268867823
10	56.65049403052698	21.019866238196826	13.677489430777218	8.652150300498981
11	50.56430198122294	17.15997707626343	21.097938964051142	11.17778197846249
12	44.190543058518536	19.289184137014335	24.302581320755284	12.217691483711846
13	20.67895066895904	41.423251990074384	25.24485491135055	12.652942429616026
14	14.101398527960058	43.604487531467626	29.149571380134965	13.14454256043735
15	13.006580072551827	20.824654418670242	52.906243717725985	13.262521791051944
16	15.080818173362259	15.702759549806618	51.18762358264397	18.028798694187152
17	16.300576994050033	16.15643349866028	29.62448879167304	37.918500715616645
18	22.42583541117202	21.29813159545006	36.229045334389504	20.046987658988417
19	29.47044368232022	23.13833154804233	27.257462966463535	20.133761803173915
20	32.02133947833497	21.179492257237932	26.306848016226645	20.492320248200457
21	21.995985345611334	26.39896303097405	30.763954524587504	20.841097098827106
22	22.48134445914684	21.557073803029894	28.70597907458916	27.255602663234107
23	18.241893428628867	29.090881813735635	26.93196991109551	25.735254846539984
24	20.04968809916016	21.90507052649582	40.592176524773535	17.45306484957048
25	16.95492365255537	23.068960240519203	38.38387657188122	21.59223953504421
26	16.393652165302942	32.8128684975651	29.606605876757914	21.18687346037404
27	17.66910006331032	32.012458030659	32.26467914270026	18.053762763330422
28	14.663450142373208	26.38372054644907	42.08972062446178	16.863108686715936
29	15.276630090704785	22.66407424410179	41.30725308225241	20.75204258294102
30	19.110775056334184	27.37310181559594	34.97010012632059	18.546023001749283
31	30.65119614497163	24.268975843062417	25.968212818689445	19.111615193276503
32	30.21654529688339	25.695948439595657	27.626703152613874	16.460803110907076
33	28.31057462366366	26.692710911878635	26.23369609246307	18.763018371994637
34	19.926007939294106	26.90184500073512	29.389790535857347	23.78235652411343
35	19.84925542863487	25.416782935618503	32.35295353142562	22.381008104321005
36	32.76324040818653	21.7981330956946	27.544609771392736	17.894016724726132
37	20.56859268060694	29.87106898424443	30.426459512900607	19.133878822248025
38	20.795909733286525	32.077208584999354	24.104969109964923	23.021912571749194
39	21.02922776412555	30.230167517305322	27.880484518976594	20.860120199592533
40	24.535779332031122	25.25685686766943	25.775341380645045	24.432022419654402
41	18.43398473951254	23.02209260109398	28.490423939102072	30.053498720291405
42	24.139714773508082	24.658859394081233	24.050300198932426	27.15112563347826
43	24.027016403673798	25.659402482604666	25.559366176686797	24.754214937034735
44	20.119779524062423	30.260412447228894	28.229201359821648	21.390606668887028
45	17.799801367622923	37.39893602657233	22.835762229243368	21.96550037656138
46	23.848307274085677	30.66181787631384	26.193009460542065	19.296865389058414
47	22.537213565811225	26.603056298176604	27.41864923982609	23.44108089618608
48	24.33690691582728	22.582760990041375	31.28225900821834	21.798073085913003
49	21.93249499668446	21.846080911188523	32.58615154270146	23.635272549425558
50	20.234098157999757	31.44014474359321	27.55295113103436	20.772805967372683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	561.0
1	1392.5
2	2224.0
3	12507.0
4	22790.0
5	17008.5
6	11227.0
7	11766.0
8	12305.0
9	13004.5
10	13704.0
11	13439.5
12	13175.0
13	12322.5
14	11470.0
15	10539.5
16	9609.0
17	8832.0
18	8055.0
19	7324.5
20	6594.0
21	6130.0
22	5666.0
23	5986.5
24	6307.0
25	6777.5
26	7248.0
27	8395.0
28	9542.0
29	10763.5
30	11985.0
31	15313.5
32	18642.0
33	22493.0
34	26344.0
35	29033.0
36	31722.0
37	36987.0
38	42252.0
39	55273.5
40	68295.0
41	101829.0
42	135363.0
43	159919.0
44	184475.0
45	179958.5
46	175442.0
47	160954.5
48	146467.0
49	141432.0
50	136397.0
51	126370.5
52	116344.0
53	105507.0
54	94670.0
55	84345.5
56	74021.0
57	66791.0
58	59561.0
59	55682.0
60	51803.0
61	49234.0
62	46665.0
63	41385.0
64	36105.0
65	30407.0
66	24709.0
67	20720.5
68	16732.0
69	13631.0
70	10530.0
71	8915.5
72	7301.0
73	5804.5
74	4308.0
75	3561.5
76	2815.0
77	2045.5
78	1276.0
79	1067.5
80	859.0
81	637.5
82	416.0
83	327.5
84	239.0
85	174.0
86	109.0
87	78.0
88	47.0
89	28.0
90	9.0
91	8.5
92	8.0
93	6.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1666395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.459714780931417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19297847198254	14.803395100261712
2	7.002254972326536	2.5851925922501335
3	2.300258254834563	1.273863339201873
4	1.2014564172356534	0.8871417113555959
5	0.800396367823692	0.7387544330859414
6	0.5767507697883058	0.6387992825984856
7	0.44888892749045267	0.5800453098854373
8	0.36037268673649636	0.5321901609594936
9	0.29312901900254207	0.4869970276321038
>10	3.6363179174419296	16.62021330150165
>50	1.8814112470693316	25.387737989373843
>100	1.301831019582861	34.077917053238004
>500	0.0032949405709509993	0.3797829527702581
>1k	3.2949405709509997E-4	0.224378493396778
>5k	0.0	0.0
>10k+	3.2949405709509997E-4	0.7835912524886666
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12883	0.7731060162806538	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3689	0.2213760843017412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017282817099187168	0.0
2	0.0	0.0	0.0	0.09481545491915182	0.0
3	0.0	0.0	0.0	0.14390345626337092	0.0
4	0.0	0.0	0.0	0.22593682770291557	0.0
5	0.0	0.0	0.0	0.4142475223461424	0.0
6	0.0	0.0	0.0	0.61648048631927	0.0
7	0.0	0.0	0.0	0.7382403331743074	0.0
8	0.0	0.0	0.0	1.0604328505546403	0.0
9	0.0	0.0	0.0	1.209257108908752	0.0
10	0.0	0.0	0.0	1.4638786122137908	0.0
11	0.0	0.0	0.0	1.6964165158920903	0.0
12	0.0	0.0	0.0	1.8961290690382533	0.0
13	6.0009781594399886E-5	0.0	0.0	1.9799627339256298	0.0
14	6.0009781594399886E-5	0.0	0.0	2.0112878399179066	0.0
15	0.006361036849006388	0.0	0.0	2.0535347261603643	0.0
16	0.006361036849006388	0.0	0.0	2.1512906603776414	0.0
17	0.006361036849006388	0.0	0.0	2.2674095877628053	0.0
18	0.006361036849006388	0.0	0.0	2.4326765262737826	0.0
19	0.006361036849006388	0.0	0.0	2.510569222783314	0.0
20	0.006421046630600788	0.0	0.0	2.600103816922158	0.0
21	0.006421046630600788	0.0	0.0	2.7209635170532795	0.0
22	0.006421046630600788	0.0	0.0	2.833541867324374	0.0
23	0.006421046630600788	0.0	0.0	2.9685038661301792	0.0
24	0.006421046630600788	0.0	0.0	3.0630792819229535	0.0
25	0.006421046630600788	0.0	0.0	3.140311870834946	0.0
26	0.006421046630600788	0.0	0.0	3.2200048607923093	0.0
27	0.006421046630600788	0.0	0.0	3.3061789071618675	0.0
28	0.006421046630600788	0.0	0.0	3.392713012220992	0.0
29	0.006421046630600788	0.0	0.0	3.4894487801511644	0.0
30	0.006421046630600788	0.0	0.0	3.6181697616711523	0.0
31	0.006421046630600788	0.0	0.0	3.7383693542047354	0.0
32	0.006421046630600788	0.0	0.0	3.835105122134908	0.0
33	0.006421046630600788	0.0	0.0	3.9344213106736396	0.0
34	0.006421046630600788	0.0	0.0	4.044479250117769	0.0
35	0.006421046630600788	0.0	0.0	4.1898829509210005	0.0
36	0.006421046630600788	0.0	0.0	4.303121408789632	0.0
37	0.006421046630600788	0.0	0.0	4.416959964474209	0.0
38	0.006421046630600788	0.0	0.0	4.54190032975375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACA	20	7.8575034E-4	44.0	1
GTACACG	40	8.3164196E-9	44.0	1
CAACGTA	20	7.8575034E-4	44.0	26
CTATACG	45	4.802132E-10	44.0	1
ATTACGT	20	7.8575034E-4	44.0	35
ATTACGC	20	7.8575034E-4	44.0	27
TCCAACG	30	2.5284062E-6	44.0	37
TTACGTT	20	7.8575034E-4	44.0	36
CGTAAGA	25	4.443706E-5	44.0	38
TCGAGGT	30	2.5284062E-6	44.0	44
CACGTAC	25	4.443706E-5	44.0	37
CAATCGA	20	7.8575034E-4	44.0	26
CAATACG	25	4.443706E-5	44.0	1
ATACCCG	20	7.8575034E-4	44.0	1
ATCGATA	40	8.3164196E-9	44.0	15
TAATCGG	45	4.802132E-10	44.0	2
CGTTTTT	10455	0.0	43.221424	1
CGACGGT	220	0.0	43.0	28
AATGCGG	255	0.0	42.27451	2
CGGTCTA	220	0.0	42.0	31
>>END_MODULE