FastQCFastQC Report
Thu 26 May 2016
SRR1545920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545920_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1413076
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116810.8266363592616393No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26710.18902026501051605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT302.5281315E-644.026
CGAGTTA254.443362E-544.032
GTCGACC302.5281315E-644.015
GTTTCGA302.5281315E-644.010
CGCATGG1100.044.02
CCGTCGA207.857096E-444.020
ATTTTCG302.5281315E-644.01
TCAGCGA207.857096E-444.021
TACGATG700.044.01
ATATACG502.7284841E-1144.01
CGGATAT207.857096E-444.013
TCCGTCG207.857096E-444.019
CCGGTGA454.802132E-1044.029
CCGCGAA207.857096E-444.026
TTGCACG254.443362E-544.01
CACGTAT207.857096E-444.024
ATACGAG700.044.01
CGGTAAC207.857096E-444.020
TCGACAT207.857096E-444.019
GTGCGTA207.857096E-444.042
Produced by FastQC (version 0.11.5)