FastQCFastQC Report
Thu 26 May 2016
SRR1545919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545919_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2211636
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197420.8926423697208762No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29440.13311412908815012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCG302.5287827E-644.0000041
CGAACTT454.802132E-1044.025
TATGCGC351.446897E-744.025
CGTAAGA207.8580633E-444.022
TAAGCGT254.4441804E-544.02
CGTTTTT145400.043.409911
ACGTAAG900.041.5555571
TTCGCGA557.8216544E-1140.015
GTAGGGC8100.038.8395084
CGTAAGG4900.038.6122442
TATACGG2000.038.52
TCTAGCG750.038.133331
TAATGCG1500.038.133331
GCGTAAT750.038.133339
CGTAAAT1100.038.016
ATTACGG1450.037.9310382
GTTTTTT172050.037.9006082
TTAGCGG4950.037.777782
AAGTACG1050.037.7142871
TGACGTA357.29326E-637.71428729
Produced by FastQC (version 0.11.5)