##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545919_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2211636
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.496883302677293	31.0	31.0	33.0	30.0	34.0
2	31.896424637689023	31.0	31.0	34.0	30.0	34.0
3	31.972276631416744	33.0	31.0	34.0	30.0	34.0
4	35.73220367185197	37.0	35.0	37.0	33.0	37.0
5	35.665573358364576	37.0	35.0	37.0	33.0	37.0
6	35.7194764418738	37.0	35.0	37.0	33.0	37.0
7	35.99476224839892	37.0	35.0	37.0	35.0	37.0
8	35.98396752449318	37.0	35.0	37.0	35.0	37.0
9	37.81401053337891	39.0	38.0	39.0	35.0	39.0
10	37.29190924727215	39.0	37.0	39.0	34.0	39.0
11	36.92813419568139	39.0	37.0	39.0	33.0	39.0
12	36.17946217189447	38.0	35.0	39.0	32.0	39.0
13	35.8576379657412	38.0	35.0	39.0	31.0	39.0
14	36.8245855104547	39.0	35.0	41.0	31.0	41.0
15	37.04557079013002	39.0	35.0	41.0	32.0	41.0
16	37.2278602808057	39.0	35.0	41.0	32.0	41.0
17	37.160150675789325	39.0	35.0	41.0	32.0	41.0
18	37.06843847721777	38.0	35.0	41.0	32.0	41.0
19	36.92585895689888	38.0	35.0	41.0	32.0	41.0
20	36.717930979600624	38.0	35.0	40.0	32.0	41.0
21	36.58892873872554	38.0	35.0	40.0	32.0	41.0
22	36.494243627794084	38.0	35.0	40.0	31.0	41.0
23	36.441371907492915	37.0	35.0	40.0	31.0	41.0
24	36.46618928250399	38.0	35.0	40.0	32.0	41.0
25	36.42961726070656	37.0	35.0	40.0	31.0	41.0
26	36.195472944010675	37.0	35.0	40.0	31.0	41.0
27	36.1720396123051	37.0	35.0	40.0	31.0	41.0
28	36.15849714871706	37.0	35.0	40.0	31.0	41.0
29	36.11609460146245	37.0	35.0	40.0	31.0	41.0
30	35.99347180096544	37.0	35.0	40.0	30.0	41.0
31	35.75367555963097	37.0	35.0	40.0	30.0	41.0
32	35.50461423127495	37.0	34.0	40.0	29.0	41.0
33	35.24071366174181	37.0	34.0	40.0	27.0	41.0
34	34.98993821768139	37.0	34.0	40.0	26.0	41.0
35	34.75636135421923	37.0	34.0	40.0	24.0	41.0
36	34.56872514283544	37.0	34.0	40.0	23.0	41.0
37	34.47256465349633	36.0	34.0	40.0	23.0	41.0
38	34.38675803794114	36.0	34.0	40.0	23.0	41.0
39	34.29645882052924	36.0	34.0	40.0	23.0	41.0
40	34.137016670012606	36.0	33.0	40.0	23.0	41.0
41	34.08728606334858	36.0	34.0	40.0	22.0	41.0
42	34.04647509807219	36.0	33.0	40.0	22.0	41.0
43	33.92162227418979	35.0	33.0	40.0	22.0	41.0
44	33.81529555496474	35.0	33.0	40.0	22.0	41.0
45	33.723606868399685	35.0	33.0	40.0	22.0	41.0
46	33.68236680900473	35.0	33.0	40.0	22.0	41.0
47	33.600489411458305	35.0	33.0	40.0	21.0	41.0
48	33.50934737904429	35.0	33.0	40.0	20.0	41.0
49	33.45046201092766	35.0	33.0	40.0	20.0	41.0
50	33.29585836005563	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	3.0
13	12.0
14	18.0
15	47.0
16	167.0
17	423.0
18	976.0
19	1955.0
20	3567.0
21	5876.0
22	9003.0
23	13470.0
24	19960.0
25	30330.0
26	42768.0
27	50768.0
28	53199.0
29	52979.0
30	57571.0
31	66960.0
32	81601.0
33	107403.0
34	194737.0
35	325788.0
36	139884.0
37	179565.0
38	277196.0
39	495403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.923274896954105	22.405450083105897	28.34033267680577	16.33094234313422
2	33.63293055457589	24.697915931916466	29.142318175323606	12.526835338184043
3	28.334364244387412	23.989028936045536	34.94548831724569	12.731118502321358
4	24.488613858700077	26.73238272482452	34.766616206283494	14.012387210191912
5	21.774243139467796	31.443691457364594	33.149623174880496	13.632442228287115
6	19.502531157930147	40.905148948561155	29.506980353005645	10.085339540503048
7	86.95183113315211	2.9674865122470426	8.9969145013013	1.0837678532995485
8	88.50113671508332	1.8323991832290665	8.63501046284289	1.0314536388447286
9	85.45795962807622	3.248635851469229	9.575762015087474	1.7176425053670679
10	48.83244801585794	27.111332967992922	14.676284885939639	9.379934130209492
11	41.13868647462783	19.84983062312243	26.361073883767496	12.650409018482245
12	37.830592375960606	20.43754035474192	27.541557471482648	14.190309797814828
13	21.59880739868586	34.99545133105086	28.36131262106423	15.044428649199054
14	16.052822435518323	36.907700905573975	31.381746363325615	15.657730295582095
15	14.783354946293151	22.483446643118487	46.97540644120461	15.75779196938375
16	17.811837029239893	18.47026364193746	44.888941941621496	18.828957387201147
17	18.544100385416044	18.709362661848512	31.37451189978821	31.37202505294723
18	22.312170718870554	22.02776587105654	35.759953265365546	19.900110144707355
19	26.801652713195118	23.768558659743285	28.897612446171067	20.532176180890527
20	29.101850394911278	22.454237496586238	28.733344908474994	19.71056720002749
21	22.240685176041627	26.728765493055818	31.10810278002348	19.922446550879076
22	22.96571406868038	22.238198329200646	29.859027434894347	24.937060167224626
23	19.254434273994455	27.767227518452405	28.99785498156116	23.980483225991982
24	19.755918243327564	23.19088674628194	39.85013808782277	17.20305692256773
25	18.294059239404675	24.325974075299914	36.403051858443256	20.97691482685216
26	17.640425458800635	30.726530043822763	30.103733164046886	21.529311333329716
27	17.492932833431905	29.90871915631686	32.529855726710906	20.06849228354033
28	16.009415654293928	25.695412807532524	39.597293587190656	18.697877950982893
29	16.816329631096618	22.750940932413833	38.53884635627201	21.89388308021754
30	19.59802607662382	27.633028219833644	34.12320110542603	18.645744598116508
31	27.78938306303569	24.86679544011763	27.901833755645143	19.441987741201537
32	28.100555425938083	24.698820239858637	28.646621776820417	18.55400255738286
33	26.718998967280328	25.703958517586077	27.62954663425627	19.947495880877323
34	19.481144275097712	26.76285790247581	30.197193389870662	23.55880443255581
35	20.215171031761102	25.34521955692528	32.05884693502909	22.380762476284524
36	27.943567567176515	24.851874359071747	27.93868430428877	19.265873769462967
37	20.618085435397145	30.067786923345434	29.287685677028225	20.026441964229193
38	20.962265038188924	30.451303921621818	26.325172858463148	22.261258181726106
39	20.630745746587596	29.987936532051386	28.109191566785857	21.272126154575165
40	24.145022056070708	25.891150261616286	26.785149093250425	23.178678589062578
41	18.778361357836463	24.054998200427193	29.173155076151776	27.99348536558457
42	22.83155998545873	24.49571267604615	25.53060268507114	27.142124653423984
43	21.902835728845073	25.949613770077896	26.79057494090348	25.356975560173556
44	19.980683982355142	28.496054504448292	28.945495551709232	22.577765961487334
45	18.58827582839129	33.47295847960514	25.081523360987067	22.857242331016497
46	22.430770705486797	30.445154627615032	26.811916608338805	20.312158058559366
47	21.101935399857844	26.790439294712147	28.890649275016322	23.216976030413687
48	22.863979425185697	24.417987408416213	31.34159509069304	21.376438075705043
49	21.055047033056074	23.997710292290414	32.11098028789547	22.836262386758037
50	20.181892499489066	29.43300796333574	28.954538631130983	21.430560906044214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	708.0
1	1943.5
2	3179.0
3	19783.5
4	36388.0
5	26325.5
6	16263.0
7	16300.0
8	16337.0
9	16720.0
10	17103.0
11	16809.5
12	16516.0
13	15474.5
14	14433.0
15	13378.5
16	12324.0
17	11569.0
18	10814.0
19	9790.5
20	8767.0
21	8743.5
22	8720.0
23	9027.0
24	9334.0
25	10767.0
26	12200.0
27	14684.0
28	17168.0
29	21468.5
30	25769.0
31	31227.0
32	36685.0
33	41700.0
34	46715.0
35	53850.5
36	60986.0
37	66246.5
38	71507.0
39	85728.5
40	99950.0
41	130560.5
42	161171.0
43	190472.5
44	219774.0
45	213849.5
46	207925.0
47	195913.5
48	183902.0
49	177564.0
50	171226.0
51	158561.5
52	145897.0
53	131447.0
54	116997.0
55	108233.5
56	99470.0
57	92773.5
58	86077.0
59	79428.0
60	72779.0
61	65480.0
62	58181.0
63	51689.0
64	45197.0
65	39338.5
66	33480.0
67	28530.5
68	23581.0
69	19933.0
70	16285.0
71	13983.5
72	11682.0
73	9443.0
74	7204.0
75	5827.5
76	4451.0
77	3298.5
78	2146.0
79	1677.5
80	1209.0
81	898.5
82	588.0
83	432.5
84	277.0
85	240.0
86	203.0
87	118.5
88	34.0
89	26.5
90	19.0
91	14.5
92	10.0
93	7.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2211636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.82494297506721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28286361677624	14.924953864858434
2	7.063439683369088	2.6593769849449975
3	2.445385111117589	1.38102705806601
4	1.3130527197082944	0.9887257028706221
5	0.9041414997574971	0.8510206087163312
6	0.6615386989222168	0.7472056969806532
7	0.4918874086980161	0.6481826693225613
8	0.39234481377984876	0.5908694996775212
9	0.3122974150703725	0.5291082926964591
>10	4.194584449160215	19.412985367862227
>50	1.7337174074131947	23.438440321879032
>100	1.2008546167191476	32.33572193830559
>500	0.003405989569668143	0.45340274163178373
>1k	2.432849692620102E-4	0.13483006781454257
>5k	0.0	0.0
>10k+	2.432849692620102E-4	0.9041491843731995
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19742	0.8926423697208762	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2944	0.13311412908815012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01971391313941354	0.0
2	0.0	0.0	0.0	0.07487669761208444	0.0
3	0.0	0.0	0.0	0.11199853863836544	0.0
4	0.0	0.0	0.0	0.17692784888652563	0.0
5	0.0	0.0	0.0	0.31334270196361425	0.0
6	0.0	0.0	0.0	0.4838047490635891	0.0
7	0.0	0.0	0.0	0.5976571189834132	0.0
8	0.0	0.0	0.0	0.9163804532029683	0.0
9	0.0	0.0	0.0	1.0584020155215415	0.0
10	0.0	0.0	0.0	1.2830773237549036	0.0
11	0.0	0.0	0.0	1.457925264374427	0.0
12	0.001537323501697386	0.0	0.0	1.614777476944669	0.0
13	0.001537323501697386	0.0	0.0	1.6782598944853493	0.0
14	0.001537323501697386	0.0	0.0	1.700822377642614	0.0
15	0.001537323501697386	0.0	0.0	1.7338748329291076	0.0
16	0.001537323501697386	0.0	0.0	1.8082993765701048	0.0
17	0.001537323501697386	0.0	0.0	1.9016691715996665	0.0
18	0.001537323501697386	0.0	0.0	2.046855811715852	0.0
19	0.001537323501697386	0.0	0.0	2.113955461025232	0.0
20	0.001537323501697386	0.0	0.0	2.18833478926912	0.0
21	0.001537323501697386	0.0	0.0	2.291245033088628	0.0
22	0.001537323501697386	0.0	0.0	2.4009828018715558	0.0
23	0.001537323501697386	0.0	0.0	2.5227478662854104	0.0
24	0.001537323501697386	0.0	0.0	2.6132238759000126	0.0
25	0.001537323501697386	0.0	0.0	2.6918986668692315	0.0
26	0.001537323501697386	0.0	0.0	2.773557674047628	0.0
27	0.001537323501697386	0.0	0.0	2.8572965894930267	0.0
28	0.0015825388988061327	0.0	0.0	2.9471395835481067	0.0
29	0.0015825388988061327	0.0	0.0	3.0465230263931318	0.0
30	0.0015825388988061327	0.0	0.0	3.186057741870724	0.0
31	0.0015825388988061327	0.0	0.0	3.304115143721661	0.0
32	0.0015825388988061327	0.0	0.0	3.4070253875411685	0.0
33	0.0015825388988061327	0.0	0.0	3.519928234121709	0.0
34	0.0015825388988061327	0.0	0.0	3.6435019144199137	0.0
35	0.0015825388988061327	0.0	0.0	3.797279479986761	0.0
36	0.0015825388988061327	0.0	0.0	3.915789035808786	0.0
37	0.0015825388988061327	0.0	0.0	4.038820131341685	0.0
38	0.0015825388988061327	0.0	0.0	4.186131895121982	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACTCG	30	2.5287827E-6	44.000004	1
CGAACTT	45	4.802132E-10	44.0	25
TATGCGC	35	1.446897E-7	44.0	25
CGTAAGA	20	7.8580633E-4	44.0	22
TAAGCGT	25	4.4441804E-5	44.0	2
CGTTTTT	14540	0.0	43.40991	1
ACGTAAG	90	0.0	41.555557	1
TTCGCGA	55	7.8216544E-11	40.0	15
GTAGGGC	810	0.0	38.839508	4
CGTAAGG	490	0.0	38.612244	2
TATACGG	200	0.0	38.5	2
TCTAGCG	75	0.0	38.13333	1
TAATGCG	150	0.0	38.13333	1
GCGTAAT	75	0.0	38.13333	9
CGTAAAT	110	0.0	38.0	16
ATTACGG	145	0.0	37.931038	2
GTTTTTT	17205	0.0	37.900608	2
TTAGCGG	495	0.0	37.77778	2
AAGTACG	105	0.0	37.714287	1
TGACGTA	35	7.29326E-6	37.714287	29
>>END_MODULE