FastQCFastQC Report
Thu 26 May 2016
SRR1545918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545918_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1799040
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99890.555240572749911No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA72720.4042155816435432No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21730.12078664176449663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA254.443846E-544.025
AACGTCA302.5285171E-644.022
CTATGCG800.044.01
AGCCGTC254.443846E-544.029
AGCCGTA600.044.036
ACGTTAG551.8189894E-1244.01
ACACCGT207.857671E-444.021
CGTTGCA207.857671E-444.040
CGCATAT551.8189894E-1244.023
ATAGCCG254.443846E-544.018
TAAGTCG302.5285171E-644.01
ATAGACG254.443846E-544.01
AGGCGTA302.5285171E-644.023
TAACGCG302.5285171E-644.01
TAGCCGA254.443846E-544.019
AATGCGG2150.044.02
CCGTAGA207.857671E-444.019
TACGCAA302.5285171E-644.08
CGTAGTA254.443846E-544.044
CGCTAAA207.857671E-444.023
Produced by FastQC (version 0.11.5)