##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545918_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1799040
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766349275168977	31.0	31.0	33.0	30.0	34.0
2	32.19369052383493	33.0	31.0	34.0	30.0	34.0
3	32.23662786819637	34.0	31.0	34.0	30.0	34.0
4	35.89293345339737	37.0	35.0	37.0	35.0	37.0
5	35.868875066702245	37.0	35.0	37.0	35.0	37.0
6	35.92213458288865	37.0	35.0	37.0	35.0	37.0
7	36.13812477765919	37.0	35.0	37.0	35.0	37.0
8	36.0904854811455	37.0	35.0	37.0	35.0	37.0
9	37.87131859213803	39.0	38.0	39.0	35.0	39.0
10	37.63420101832088	39.0	37.0	39.0	35.0	39.0
11	37.32411563945215	39.0	37.0	39.0	35.0	39.0
12	36.099516964603346	37.0	35.0	39.0	33.0	39.0
13	35.71155949839915	37.0	35.0	39.0	32.0	39.0
14	36.57801772056208	38.0	35.0	41.0	33.0	41.0
15	36.853890408217715	38.0	35.0	41.0	33.0	41.0
16	37.01071571504803	38.0	35.0	41.0	33.0	41.0
17	36.97268654393454	38.0	35.0	41.0	33.0	41.0
18	36.94884049270722	37.0	35.0	41.0	33.0	41.0
19	36.847356367840625	37.0	35.0	40.0	33.0	41.0
20	36.656499577552474	36.0	35.0	40.0	33.0	41.0
21	36.486888006937036	36.0	35.0	40.0	33.0	41.0
22	36.46497520900036	36.0	35.0	40.0	32.0	41.0
23	36.47317680540733	35.0	35.0	40.0	33.0	41.0
24	36.419943970117394	35.0	35.0	40.0	33.0	41.0
25	36.33739549982213	35.0	35.0	40.0	33.0	41.0
26	36.22507893098541	35.0	35.0	40.0	32.0	41.0
27	36.135706821415866	35.0	35.0	40.0	32.0	41.0
28	36.17236192636073	36.0	35.0	40.0	32.0	41.0
29	36.1729411241551	36.0	35.0	40.0	32.0	41.0
30	36.07442302561366	36.0	35.0	40.0	32.0	41.0
31	35.852888207043755	36.0	35.0	40.0	31.0	41.0
32	35.65014229811455	35.0	35.0	40.0	31.0	41.0
33	35.46113983013163	35.0	35.0	40.0	30.0	41.0
34	35.33538331554607	35.0	35.0	40.0	30.0	41.0
35	35.12271544823906	35.0	35.0	40.0	29.0	41.0
36	34.935835779082176	35.0	35.0	40.0	28.0	41.0
37	34.85578475186766	35.0	34.0	40.0	28.0	41.0
38	34.82259538420491	35.0	34.0	40.0	28.0	41.0
39	34.83623376912131	35.0	34.0	40.0	28.0	41.0
40	34.61659996442547	35.0	34.0	40.0	26.0	41.0
41	34.58111326040555	35.0	34.0	40.0	27.0	41.0
42	34.54304907061544	35.0	34.0	40.0	27.0	41.0
43	34.475036130380644	35.0	34.0	40.0	27.0	41.0
44	34.34516909018143	35.0	34.0	40.0	26.0	41.0
45	34.24144265830665	35.0	34.0	40.0	25.0	41.0
46	34.2320198550338	35.0	34.0	40.0	26.0	41.0
47	34.18465904037709	35.0	34.0	40.0	26.0	41.0
48	34.13899023923871	35.0	34.0	40.0	25.0	41.0
49	34.12002568036286	35.0	34.0	39.0	24.0	41.0
50	33.880072705442906	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	4.0
13	4.0
14	10.0
15	27.0
16	91.0
17	230.0
18	575.0
19	1258.0
20	2290.0
21	3792.0
22	5620.0
23	8579.0
24	12250.0
25	18295.0
26	25372.0
27	30944.0
28	32893.0
29	34317.0
30	38872.0
31	46504.0
32	59294.0
33	81554.0
34	176814.0
35	429748.0
36	92232.0
37	103784.0
38	176793.0
39	416888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.440635005336176	24.058164354322304	29.463602810387762	14.037597829953752
2	32.125077819281394	25.100498043400925	30.365083600142302	12.409340537175382
3	29.023590359302737	24.844583778014943	32.461145944503734	13.670679918178585
4	26.216815635005336	26.65293712202063	31.531650213447172	15.598597029526859
5	23.23122331910352	31.758938100320172	30.85167644966204	14.158162130914265
6	20.87313233724653	39.478054962646745	28.8650613660619	10.783751334044824
7	84.97337468872287	5.405994308075418	7.792711668445393	1.8279193347563143
8	86.71408084311632	4.087457755247243	7.348641497687655	1.8498199039487726
9	83.18114105300604	5.316446549270722	8.857779704731412	2.644632692991818
10	59.0937388829598	18.23783795802206	13.484469494841694	9.18395366417645
11	55.25357968694414	17.616673336890788	17.980033795802207	9.14971318036286
12	48.00210112059765	21.33988127001067	20.449461935254355	10.208555674137317
13	19.722907773034507	48.416933475631446	21.314867929562435	10.545290821771612
14	12.660085378868729	49.62880202774813	26.68150791533262	11.029604678050516
15	11.50313500533618	22.077774813233724	55.55757515119175	10.86151503023835
16	12.813000266808967	16.019488171469227	53.2470095161864	17.920502045535397
17	13.194481501245109	16.42159151547492	30.162030860903595	40.22189612237638
18	20.31322260761295	21.986059231590183	38.03989905727499	19.660819103521877
19	31.99017253646389	22.02596940590537	27.13608368907862	18.847774368552116
20	34.16388740661686	21.765441568836714	24.906616862326576	19.164054162219852
21	20.528281750266807	29.624355211668448	28.950217893987904	20.89714514407684
22	21.484291622198505	24.944692725008892	24.99232924226254	28.578686410530064
23	17.710890252579155	31.74921069014586	25.509938633938102	25.029960423336888
24	17.82300560298826	22.931563500533617	43.97373043400925	15.271700462468873
25	14.066168623265742	25.157639630024903	41.14083066524368	19.63536108146567
26	13.252679206688011	38.83387806830309	28.95866684453931	18.954775880469583
27	16.031438989683387	37.53179473496976	30.664409907506222	15.772356367840626
28	12.127912664532195	28.88696193525436	44.05010450017787	14.935020900035575
29	12.69871709356101	24.34742974030594	42.090614994663824	20.863238171469227
30	16.213869619352543	32.80127178939879	33.9232590715048	17.061599519743865
31	31.844261383849165	25.672469761650657	25.366528815368195	17.11674003913198
32	32.96435876912131	25.85868018498755	27.213013607257203	13.963947438633939
33	29.464269832799715	26.949428584133763	25.715937388829595	17.870364194236927
34	18.50031127712558	27.441579953753113	29.30640786197083	24.751700907150482
35	19.806619085734614	24.511406083244395	33.442447082888656	22.23952774813234
36	33.053962113127	23.63254847029527	27.046258004268942	16.267231412308785
37	19.012528904304517	34.33164354322305	30.305496264674492	16.350331287797935
38	18.953330665243687	36.27429073283529	23.899413020277482	20.872965581643545
39	19.57399501956599	33.962557808609034	28.054184453930986	18.409262717893988
40	24.448483635716826	26.119152436855213	25.856456776947706	23.575907150480255
41	15.85879135538954	23.644666044112416	28.087535574528637	32.4090070259694
42	21.6548270188545	24.268220828886516	24.803784240483814	29.273167911775168
43	22.062099786552828	25.604766986837426	26.464725631447884	25.868407595161862
44	18.237282106012096	31.454331198861613	30.115783973674848	20.19260272145144
45	15.09221584845251	42.432130469583775	21.875889363215936	20.599764318747777
46	21.078186143721094	35.257804162219855	25.565579420135183	18.09843027392387
47	21.422202952685875	27.65030238349342	27.32829731412309	23.59919734969762
48	23.033062077552472	23.396756047669868	33.34884160441124	20.22134027036642
49	21.27429073283529	23.341559943080753	34.74664265385984	20.63750667022412
50	18.606923692636073	36.55032684098185	26.280738616150835	18.562010850231232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2841.0
1	3832.5
2	4824.0
3	11342.5
4	17861.0
5	13375.5
6	8890.0
7	9161.5
8	9433.0
9	9757.0
10	10081.0
11	9840.5
12	9600.0
13	9100.0
14	8600.0
15	7975.0
16	7350.0
17	6840.5
18	6331.0
19	6295.0
20	6259.0
21	6110.0
22	5961.0
23	6221.5
24	6482.0
25	8463.0
26	10444.0
27	13009.0
28	15574.0
29	21238.5
30	26903.0
31	29149.0
32	31395.0
33	31058.5
34	30722.0
35	36160.0
36	41598.0
37	46457.5
38	51317.0
39	72606.5
40	93896.0
41	138493.0
42	183090.0
43	201560.0
44	220030.0
45	228208.5
46	236387.0
47	213987.5
48	191588.0
49	169713.5
50	147839.0
51	130159.5
52	112480.0
53	101619.5
54	90759.0
55	79418.5
56	68078.0
57	56920.0
58	45762.0
59	40329.5
60	34897.0
61	28744.0
62	22591.0
63	18900.5
64	15210.0
65	12453.0
66	9696.0
67	7213.5
68	4731.0
69	4239.0
70	3747.0
71	2969.0
72	2191.0
73	2021.0
74	1851.0
75	1326.5
76	802.0
77	658.5
78	515.0
79	363.0
80	211.0
81	149.0
82	87.0
83	73.0
84	59.0
85	51.0
86	43.0
87	29.5
88	16.0
89	15.0
90	14.0
91	9.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1799040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.413471579095646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80937372970669	11.647436115776456
2	7.415044183978137	2.1375305720701467
3	2.430208822182441	1.0508323736938237
4	1.148021889727913	0.6618792351909178
5	0.6996816093198646	0.5042420495173885
6	0.4991991951670642	0.431711604710874
7	0.3850987490020035	0.3885426911719966
8	0.2991675959342821	0.3449634913108116
9	0.2494760180154933	0.32362339457990397
>10	2.8881876384598977	9.895219661938441
>50	1.0114261079089264	10.602789521747791
>100	2.135492732603519	58.34118416552895
>500	0.027672930099443303	2.425086988311649
>1k	0.0011692787365961957	0.2752716420201788
>5k	7.795191577307972E-4	0.9696864924306747
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9989	0.555240572749911	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7272	0.4042155816435432	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2173	0.12078664176449663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.55852009960868E-5	0.0	0.0	0.030460690145855566	0.0
2	5.55852009960868E-5	0.0	0.0	0.16119708288865173	0.0
3	5.55852009960868E-5	0.0	0.0	0.2379602454642476	0.0
4	5.55852009960868E-5	0.0	0.0	0.36330487371042336	0.0
5	5.55852009960868E-5	0.0	0.0	0.6334489505514052	0.0
6	5.55852009960868E-5	0.0	0.0	0.8930318392031306	0.0
7	5.55852009960868E-5	0.0	0.0	1.0520055140519389	0.0
8	5.55852009960868E-5	0.0	0.0	1.3900747065101386	0.0
9	5.55852009960868E-5	0.0	0.0	1.5411552828175026	0.0
10	5.55852009960868E-5	0.0	0.0	1.7843961223763785	0.0
11	1.111704019921736E-4	0.0	0.0	2.017909551760939	0.0
12	1.111704019921736E-4	0.0	0.0	2.2116239772323016	0.0
13	1.111704019921736E-4	0.0	0.0	2.2897211846318037	0.0
14	1.111704019921736E-4	0.0	0.0	2.3151236214870154	0.0
15	1.111704019921736E-4	0.0	0.0	2.358591248665955	0.0
16	1.111704019921736E-4	0.0	0.0	2.4669268054073283	0.0
17	1.111704019921736E-4	0.0	0.0	2.587769032372821	0.0
18	1.111704019921736E-4	0.0	0.0	2.794545980078264	0.0
19	1.111704019921736E-4	0.0	0.0	2.863860725720384	0.0
20	1.111704019921736E-4	0.0	0.0	2.9417355923159016	0.0
21	1.111704019921736E-4	0.0	0.0	3.047069548203486	0.0
22	1.111704019921736E-4	0.0	0.0	3.1420646567057986	0.0
23	1.111704019921736E-4	0.0	0.0	3.2662419957310567	0.0
24	1.111704019921736E-4	0.0	0.0	3.3472852187833513	0.0
25	1.111704019921736E-4	0.0	0.0	3.4101520811099255	0.0
26	1.111704019921736E-4	0.0	0.0	3.4767431519032375	0.0
27	1.111704019921736E-4	0.0	0.0	3.5385538954108857	0.0
28	1.111704019921736E-4	0.0	0.0	3.605311721807186	0.0
29	1.111704019921736E-4	0.0	0.0	3.6819637139807897	0.0
30	1.111704019921736E-4	0.0	0.0	3.7894099075062257	0.0
31	1.111704019921736E-4	0.0	0.0	3.8918534329420136	0.0
32	1.111704019921736E-4	0.0	0.0	3.9762873532550693	0.0
33	1.111704019921736E-4	0.0	0.0	4.055051583066525	0.0
34	1.111704019921736E-4	0.0	0.0	4.142820615439345	0.0
35	1.111704019921736E-4	0.0	0.0	4.268776680896478	0.0
36	1.111704019921736E-4	0.0	0.0	4.367384827463536	0.0
37	1.111704019921736E-4	0.0	0.0	4.460323283528993	0.0
38	1.111704019921736E-4	0.0	0.0	4.54531305585201	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	4.443846E-5	44.0	25
AACGTCA	30	2.5285171E-6	44.0	22
CTATGCG	80	0.0	44.0	1
AGCCGTC	25	4.443846E-5	44.0	29
AGCCGTA	60	0.0	44.0	36
ACGTTAG	55	1.8189894E-12	44.0	1
ACACCGT	20	7.857671E-4	44.0	21
CGTTGCA	20	7.857671E-4	44.0	40
CGCATAT	55	1.8189894E-12	44.0	23
ATAGCCG	25	4.443846E-5	44.0	18
TAAGTCG	30	2.5285171E-6	44.0	1
ATAGACG	25	4.443846E-5	44.0	1
AGGCGTA	30	2.5285171E-6	44.0	23
TAACGCG	30	2.5285171E-6	44.0	1
TAGCCGA	25	4.443846E-5	44.0	19
AATGCGG	215	0.0	44.0	2
CCGTAGA	20	7.857671E-4	44.0	19
TACGCAA	30	2.5285171E-6	44.0	8
CGTAGTA	25	4.443846E-5	44.0	44
CGCTAAA	20	7.857671E-4	44.0	23
>>END_MODULE