FastQCFastQC Report
Thu 26 May 2016
SRR1545917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545917_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3344503
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216750.6480783542427678No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38430.1149049649529392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC351.447188E-744.021
CGTTTTT136000.042.3500021
CACATCG603.6379788E-1240.33333618
CTATACG1200.040.3333361
GACCGAT11050.039.6199079
CGTAAGG5600.039.2857132
TGGGCGA13950.038.7956966
GGACCGA11850.038.6160358
TGTAGCG2400.038.5000041
TATTGCG1950.038.3589741
CGACGGT1550.038.32258228
TATAGCG2900.037.9310381
CTACGGG6100.037.8688553
TCGCCTA357.2943785E-637.71428712
CGTCGGA357.2943785E-637.71428721
ACTGCGG5250.037.7142832
AAGGGAT73900.037.3910685
CGCGAGG2650.037.3584942
GGGACCG14950.037.230777
TCGAACG651.0913936E-1137.230771
Produced by FastQC (version 0.11.5)