##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545917_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3344503
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73858537426936	31.0	31.0	33.0	30.0	34.0
2	32.17221811432072	33.0	31.0	34.0	30.0	34.0
3	32.213706789917666	34.0	31.0	34.0	30.0	34.0
4	35.898259920831286	37.0	35.0	37.0	35.0	37.0
5	35.89416125505045	37.0	35.0	37.0	35.0	37.0
6	35.946067323007334	37.0	35.0	37.0	35.0	37.0
7	36.16764164959637	37.0	35.0	37.0	35.0	37.0
8	36.143182111064036	37.0	35.0	37.0	35.0	37.0
9	37.961569476840054	39.0	39.0	39.0	35.0	39.0
10	37.63231457708365	39.0	37.0	39.0	35.0	39.0
11	37.350732530364006	39.0	37.0	39.0	34.0	39.0
12	36.96354406020865	39.0	35.0	39.0	33.0	39.0
13	36.85324336680218	39.0	35.0	39.0	33.0	39.0
14	37.999549708880515	40.0	36.0	41.0	33.0	41.0
15	38.13532234834294	40.0	36.0	41.0	33.0	41.0
16	38.1631572762829	40.0	36.0	41.0	34.0	41.0
17	38.08833748990508	40.0	36.0	41.0	34.0	41.0
18	37.98706863172196	40.0	36.0	41.0	34.0	41.0
19	37.92355276703295	40.0	36.0	41.0	34.0	41.0
20	37.81098835910747	40.0	35.0	41.0	34.0	41.0
21	37.709705747012336	40.0	35.0	41.0	33.0	41.0
22	37.706731912035956	40.0	35.0	41.0	33.0	41.0
23	37.6795517899072	40.0	35.0	41.0	33.0	41.0
24	37.65088385329599	40.0	35.0	41.0	33.0	41.0
25	37.58890872575088	40.0	35.0	41.0	33.0	41.0
26	37.50839930476965	40.0	35.0	41.0	33.0	41.0
27	37.42815360010142	40.0	35.0	41.0	33.0	41.0
28	37.39637488738985	39.0	35.0	41.0	33.0	41.0
29	37.34624098109644	39.0	35.0	41.0	33.0	41.0
30	37.237106679228575	39.0	35.0	41.0	33.0	41.0
31	37.108390394626646	39.0	35.0	41.0	32.0	41.0
32	36.94684053206112	39.0	35.0	41.0	32.0	41.0
33	36.79077160343405	39.0	35.0	41.0	31.0	41.0
34	36.63203830285098	39.0	35.0	41.0	31.0	41.0
35	36.44254617203214	39.0	35.0	41.0	30.0	41.0
36	36.32613126673829	39.0	35.0	41.0	30.0	41.0
37	36.273434049842386	39.0	35.0	41.0	30.0	41.0
38	36.197277742014286	39.0	35.0	41.0	30.0	41.0
39	36.15416042383577	39.0	35.0	41.0	30.0	41.0
40	36.03850736566838	39.0	35.0	41.0	29.0	41.0
41	35.91910188150526	39.0	35.0	41.0	29.0	41.0
42	35.89767089459929	39.0	35.0	41.0	29.0	41.0
43	35.853655087168406	39.0	35.0	41.0	29.0	41.0
44	35.74056504060543	39.0	35.0	41.0	28.0	41.0
45	35.640140254022796	38.0	35.0	40.0	27.0	41.0
46	35.60074127605806	38.0	35.0	40.0	27.0	41.0
47	35.531966932007535	38.0	35.0	40.0	27.0	41.0
48	35.47634461682349	38.0	35.0	40.0	27.0	41.0
49	35.41916093362751	38.0	35.0	40.0	27.0	41.0
50	35.27568819642261	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	3.0
13	7.0
14	19.0
15	40.0
16	129.0
17	315.0
18	754.0
19	1633.0
20	2937.0
21	4898.0
22	8054.0
23	12194.0
24	18986.0
25	28791.0
26	41244.0
27	49828.0
28	51761.0
29	53536.0
30	59334.0
31	70208.0
32	87253.0
33	114483.0
34	202898.0
35	387166.0
36	192032.0
37	261091.0
38	475472.0
39	1219429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.03555117157915	22.66273942645589	29.5281840082069	14.773525393758055
2	31.955091683278503	24.78783843219755	30.537840749432725	12.719229135091222
3	28.23394088748014	24.365443834255792	34.16731873166207	13.233296546601991
4	24.830415759830384	27.203533678995058	33.568036865268176	14.398013695906386
5	22.177345931518076	32.08883352773192	31.93021504241437	13.803605498335628
6	20.672817455986735	40.465623741404926	28.674095971808068	10.187462830800271
7	88.60443539742676	3.202837611447799	6.986389308067596	1.2063376830578414
8	90.3423617799117	2.227326451792688	6.361513205400025	1.0687985628955932
9	85.89733661473767	4.847416791074787	7.519682296592349	1.7355642975951884
10	41.48798790134139	37.36899025056937	13.288132795814505	7.854889052274733
11	30.330874273397274	23.09174188212718	32.57709740430791	14.000286440167642
12	30.17401987679485	23.303910924881812	31.453432692391065	15.06863650593227
13	20.742424210712322	33.171356102835006	30.65325401113409	15.432965675318574
14	16.42800141007498	35.19093868356524	31.836299743190544	16.544760163169236
15	15.878712023879181	26.65726417348108	41.40154755430029	16.06247624833944
16	18.616159112430157	24.434721691085343	39.443319381085914	17.505799815398582
17	18.74311370030166	23.78233776438532	32.12629798807177	25.348250547241246
18	20.580875544139143	24.42778493545977	35.67806636740945	19.313273152991638
19	23.751630660818662	25.465457797466467	31.31430888236608	19.46860265934879
20	25.87795555871829	25.34310778013953	30.919481908074236	17.859454753067943
21	21.920536474328173	27.170225292068807	32.20816366437704	18.70107456922598
22	21.490607124586226	24.59193488539254	31.437226996058904	22.480230993962333
23	18.72475521774087	28.3713903082162	31.807775325661243	21.096079148381687
24	17.502959333569144	25.754050751337342	39.367164568248256	17.375825346845257
25	17.23735933261235	28.23229639799994	35.457465578592696	19.072878690795015
26	17.79717345148143	32.13912500601734	31.16412214311065	18.899579399390582
27	17.2901025952137	31.768756075267383	32.475168956344184	18.46597237317473
28	16.133548093692845	28.295863391361888	37.620268243144054	17.95032027180122
29	17.075780766230437	25.10671989231285	36.9422302805529	20.87526906090382
30	18.26686954683551	29.188342782171222	33.747645016314834	18.79714265467844
31	23.57348760039982	27.44620052665523	30.720379081735018	18.259932791209934
32	24.366131529856602	27.254901550394784	30.661865156048595	17.71710176370002
33	23.629938439283805	27.911441550508403	29.40882397175305	19.04979603845474
34	19.04459347173556	27.616868634891347	31.931530633998534	21.40700725937456
35	19.333904021015975	27.190258163918525	32.90802848734177	20.56780932772373
36	23.885791102594318	27.626765471581276	30.41199245448427	18.07545097134014
37	19.576690467911074	31.520318564522142	30.873286703584956	18.029704263981824
38	19.214454285135936	30.83749663253404	30.670536100580563	19.277512981749457
39	19.409909334809985	31.620423124153273	30.394291767715565	18.575375773321177
40	21.75462243568028	28.153331003141574	29.847603664879358	20.244442896298793
41	18.116802406815	27.037589740538433	30.80936091251824	24.036246940128326
42	20.01541036141992	27.45905744440953	29.408524973665745	23.11700722050481
43	20.524933002003586	27.51338539687362	29.62694307644514	22.334738524677658
44	19.55806288707171	29.72600114277069	30.764780297700433	19.951155672457162
45	17.808744677460297	33.67020451170174	28.56071589710041	19.960334913737558
46	19.654878467742442	31.472927367683628	29.555273234917117	19.316920929656813
47	19.49847256827098	29.029694397044942	29.891287285435236	21.58054574924884
48	19.924544842686643	27.135631213367127	32.852325143676055	20.087498800270176
49	20.006679617270485	27.045961686983087	33.09125451524487	19.856104180501557
50	18.99304620148345	31.50979383184886	30.405683594842042	19.09147637182565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2388.0
1	4206.0
2	6024.0
3	23819.0
4	41614.0
5	30018.0
6	18422.0
7	17858.0
8	17294.0
9	17191.0
10	17088.0
11	16477.5
12	15867.0
13	15191.5
14	14516.0
15	13889.5
16	13263.0
17	13369.5
18	13476.0
19	13528.0
20	13580.0
21	15392.5
22	17205.0
23	19913.0
24	22621.0
25	29432.0
26	36243.0
27	44505.5
28	52768.0
29	63769.0
30	74770.0
31	87184.5
32	99599.0
33	115716.5
34	131834.0
35	149346.0
36	166858.0
37	173364.5
38	179871.0
39	205221.0
40	230571.0
41	254865.5
42	279160.0
43	302725.0
44	326290.0
45	318052.0
46	309814.0
47	298199.0
48	286584.0
49	266393.0
50	246202.0
51	226177.5
52	206153.0
53	182956.5
54	159760.0
55	138698.0
56	117636.0
57	99496.0
58	81356.0
59	70030.5
60	58705.0
61	47226.5
62	35748.0
63	28686.0
64	21624.0
65	17032.0
66	12440.0
67	9685.0
68	6930.0
69	5522.5
70	4115.0
71	3459.0
72	2803.0
73	2146.0
74	1489.0
75	1150.0
76	811.0
77	652.5
78	494.0
79	367.5
80	241.0
81	183.0
82	125.0
83	97.0
84	69.0
85	55.0
86	41.0
87	32.5
88	24.0
89	16.0
90	8.0
91	6.0
92	4.0
93	4.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3344503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.193362789058753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1720869883484	11.095247906034881
2	6.865042391768069	1.9487607445726363
3	2.356783180084745	1.0035203607028313
4	1.191397550482394	0.6763975063997023
5	0.8415819470277155	0.5972438945443397
6	0.6121237498658001	0.5212856672186614
7	0.4640568296522337	0.4610568856596214
8	0.3779946495153287	0.4292012154315337
9	0.34738935250048053	0.4437560798185981
>10	4.854584072164999	16.805337897488574
>50	1.925001751959535	19.92616192285938
>100	1.9870396533591899	44.86534791773881
>500	0.003848778212280607	0.3373223762249084
>1k	8.552840471734681E-4	0.23155823037301404
>5k	0.0	0.0
>10k+	2.1382101179336704E-4	0.6578013949325138
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21675	0.6480783542427678	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3843	0.1149049649529392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9799617461847095E-5	0.0	0.0	0.016385095184546103	0.0
2	5.9799617461847095E-5	0.0	0.0	0.053879455333124236	0.0
3	8.969942619277065E-5	0.0	0.0	0.07893549504963816	0.0
4	8.969942619277065E-5	0.0	0.0	0.11959923492369419	0.0
5	8.969942619277065E-5	0.0	0.0	0.21402283089595076	0.0
6	8.969942619277065E-5	0.0	0.0	0.3237850287471711	0.0
7	1.1959923492369419E-4	0.0	0.0	0.40017904005468075	0.0
8	1.1959923492369419E-4	0.0	0.0	0.5929132071342139	0.0
9	1.1959923492369419E-4	0.0	0.0	0.6735828910902457	0.0
10	1.1959923492369419E-4	0.0	0.0	0.8014643730324057	0.0
11	1.1959923492369419E-4	0.0	0.0	0.9152630450623007	0.0
12	1.1959923492369419E-4	0.0	0.0	1.019254579828453	0.0
13	1.1959923492369419E-4	0.0	0.0	1.0562406432286053	0.0
14	1.1959923492369419E-4	0.0	0.0	1.0713998462551835	0.0
15	1.1959923492369419E-4	0.0	0.0	1.0930174079676411	0.0
16	1.1959923492369419E-4	0.0	0.0	1.1412756992593518	0.0
17	1.1959923492369419E-4	0.0	0.0	1.2066366811451508	0.0
18	1.1959923492369419E-4	0.0	0.0	1.2971733019823872	0.0
19	1.1959923492369419E-4	0.0	0.0	1.345371793656636	0.0
20	1.1959923492369419E-4	0.0	0.0	1.3961117690730132	0.0
21	1.1959923492369419E-4	0.0	0.0	1.4637750362310933	0.0
22	1.1959923492369419E-4	0.0	0.0	1.5387039569107877	0.0
23	1.1959923492369419E-4	0.0	0.0	1.6251742037606185	0.0
24	1.1959923492369419E-4	0.0	0.0	1.691820877421847	0.0
25	1.1959923492369419E-4	0.0	0.0	1.7480026180272525	0.0
26	1.1959923492369419E-4	0.0	0.0	1.8046926553810836	0.0
27	1.1959923492369419E-4	0.0	0.0	1.8602763998118703	0.0
28	1.1959923492369419E-4	0.0	0.0	1.9228566994856935	0.0
29	1.1959923492369419E-4	0.0	0.0	1.994915238527219	0.0
30	1.1959923492369419E-4	0.0	0.0	2.091551420345564	0.0
31	1.1959923492369419E-4	0.0	0.0	2.1719221062142866	0.0
32	1.1959923492369419E-4	0.0	0.0	2.2505586031766156	0.0
33	1.1959923492369419E-4	0.0	0.0	2.328627003773057	0.0
34	1.1959923492369419E-4	0.0	0.0	2.414977651387964	0.0
35	1.1959923492369419E-4	0.0	0.0	2.520224978120815	0.0
36	1.1959923492369419E-4	0.0	0.0	2.615635267781192	0.0
37	1.1959923492369419E-4	0.0	0.0	2.7076967788637054	0.0
38	1.1959923492369419E-4	0.0	0.0	2.8005057851644923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACC	35	1.447188E-7	44.0	21
CGTTTTT	13600	0.0	42.350002	1
CACATCG	60	3.6379788E-12	40.333336	18
CTATACG	120	0.0	40.333336	1
GACCGAT	1105	0.0	39.619907	9
CGTAAGG	560	0.0	39.285713	2
TGGGCGA	1395	0.0	38.795696	6
GGACCGA	1185	0.0	38.616035	8
TGTAGCG	240	0.0	38.500004	1
TATTGCG	195	0.0	38.358974	1
CGACGGT	155	0.0	38.322582	28
TATAGCG	290	0.0	37.931038	1
CTACGGG	610	0.0	37.868855	3
TCGCCTA	35	7.2943785E-6	37.714287	12
CGTCGGA	35	7.2943785E-6	37.714287	21
ACTGCGG	525	0.0	37.714283	2
AAGGGAT	7390	0.0	37.391068	5
CGCGAGG	265	0.0	37.358494	2
GGGACCG	1495	0.0	37.23077	7
TCGAACG	65	1.0913936E-11	37.23077	1
>>END_MODULE