FastQCFastQC Report
Thu 26 May 2016
SRR1545916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545916_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2733534
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242660.8877153165096904No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA48480.17735283336516025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA207.8583875E-444.00000413
TCAATCG207.8583875E-444.0000041
CTTACGT207.8583875E-444.00000435
CCCGTAT600.044.021
GTCGGTA351.4470606E-743.99999622
CGTTTTT131650.041.1257131
CGGTCTA2900.040.20689431
TAATGCG557.8216544E-1140.01
ACGGGTA4550.039.6483545
TCGATAG1000.039.6000021
ATACGAC501.3496901E-939.60000217
TCGTTGA2800.039.2857124
CGTTGAT2800.039.2857125
CGTAAGG3900.038.9230772
CGCTAGC404.1273233E-738.50000432
GCCAGTA16250.038.31384325
TCACGAC3200.037.81250425
TAGCATA16450.037.71428730
AGTACGG3150.037.7142872
ACGTAAC357.293893E-637.71428316
Produced by FastQC (version 0.11.5)