##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545915_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1045806
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.787683375310525	31.0	31.0	33.0	30.0	34.0
2	32.20158614504029	33.0	31.0	34.0	30.0	34.0
3	32.224131435467	34.0	31.0	34.0	30.0	34.0
4	35.89568906661465	37.0	35.0	37.0	35.0	37.0
5	35.87420993951077	37.0	35.0	37.0	35.0	37.0
6	35.92480058442962	37.0	35.0	37.0	35.0	37.0
7	36.15520469379598	37.0	35.0	37.0	35.0	37.0
8	36.113088852043305	37.0	35.0	37.0	35.0	37.0
9	37.91305557627323	39.0	39.0	39.0	35.0	39.0
10	37.702906657640135	39.0	38.0	39.0	35.0	39.0
11	37.33352935439269	39.0	37.0	39.0	35.0	39.0
12	35.93476323524631	37.0	35.0	39.0	33.0	39.0
13	35.49264395117259	35.0	35.0	39.0	32.0	39.0
14	36.302654603243816	36.0	35.0	41.0	32.0	41.0
15	36.62606927097377	36.0	35.0	41.0	33.0	41.0
16	36.79691740150659	36.0	35.0	41.0	33.0	41.0
17	36.76782883249857	36.0	35.0	41.0	33.0	41.0
18	36.755022442020795	36.0	35.0	40.0	33.0	41.0
19	36.61778283926464	36.0	35.0	40.0	33.0	41.0
20	36.39034390699614	36.0	35.0	40.0	33.0	41.0
21	36.228964071730324	35.0	35.0	40.0	32.0	41.0
22	36.18435637202311	35.0	35.0	40.0	32.0	41.0
23	36.23511435199263	35.0	35.0	40.0	33.0	41.0
24	36.17318986504189	35.0	35.0	40.0	33.0	41.0
25	36.08195497061597	35.0	35.0	40.0	32.0	41.0
26	35.96035689219607	35.0	35.0	40.0	32.0	41.0
27	35.86155940968019	35.0	35.0	40.0	32.0	41.0
28	35.91181729689828	36.0	35.0	40.0	32.0	41.0
29	35.927384237611946	36.0	35.0	40.0	32.0	41.0
30	35.852942132670876	36.0	35.0	40.0	32.0	41.0
31	35.5742929376959	35.0	35.0	40.0	31.0	41.0
32	35.334424357863696	35.0	35.0	40.0	31.0	41.0
33	35.1625779542286	35.0	35.0	40.0	30.0	41.0
34	35.0157103707571	35.0	35.0	40.0	30.0	41.0
35	34.83403614054615	35.0	34.0	40.0	29.0	41.0
36	34.62141257556373	35.0	34.0	40.0	27.0	41.0
37	34.521674191962944	35.0	34.0	40.0	27.0	41.0
38	34.50227958149026	35.0	34.0	40.0	27.0	41.0
39	34.49016930482327	35.0	34.0	40.0	27.0	41.0
40	34.30342147587602	35.0	34.0	40.0	25.0	41.0
41	34.264597831720224	35.0	34.0	40.0	25.0	41.0
42	34.226065828652736	35.0	34.0	40.0	25.0	41.0
43	34.143276095184	35.0	34.0	40.0	26.0	41.0
44	34.03928166409449	35.0	34.0	40.0	25.0	41.0
45	33.948106054086516	35.0	34.0	39.0	24.0	41.0
46	33.953885328636474	35.0	34.0	39.0	24.0	41.0
47	33.91911023650658	35.0	34.0	39.0	24.0	41.0
48	33.89488490217115	35.0	34.0	39.0	24.0	41.0
49	33.884222312742516	35.0	34.0	39.0	24.0	41.0
50	33.64741070523596	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	4.0
15	17.0
16	75.0
17	150.0
18	410.0
19	781.0
20	1391.0
21	2414.0
22	3642.0
23	5390.0
24	7825.0
25	11164.0
26	15972.0
27	19313.0
28	20161.0
29	20449.0
30	23180.0
31	27180.0
32	34849.0
33	47769.0
34	110395.0
35	277013.0
36	49513.0
37	55068.0
38	91719.0
39	219960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89271623991448	23.124269702028865	28.895799029647947	15.08721502840871
2	31.952484495212307	25.775143764713533	30.010154847074887	12.262216892999275
3	28.692319608034378	24.723227826193387	34.16168964415963	12.422762921612613
4	26.184397488635557	26.977087528662103	32.80034729194516	14.038167690757177
5	23.295525173885025	32.39606580952873	31.622117295177116	12.686291721409132
6	20.881884403034597	40.04595498591517	28.832020470335802	10.240140140714434
7	85.4287506478257	5.157266261620224	7.920493858325541	1.4934892322285394
8	87.79333834382285	3.5866116660260126	7.338932842228865	1.2811171479222723
9	84.10594316727959	5.100372344392746	8.645293677794925	2.1483908105327374
10	63.85782831615041	16.36546357546237	12.535498935749079	7.241209172638137
11	60.19233012623756	14.943784984978093	16.702619797553275	8.16126509123107
12	51.42339975100545	20.895653687203936	18.436689022629434	9.244257539161183
13	18.40570813324842	52.497308296184954	19.40809289677053	9.688890673796097
14	11.377540385119229	52.394994865204445	26.731822154395747	9.495642595280577
15	10.418471494713168	20.79620885709204	58.94888726972306	9.836432378471724
16	10.944190413900857	14.52439553798697	56.6926370665305	17.83877698158167
17	12.031390143104934	15.56110789190347	29.53635760360908	42.87114436138251
18	20.61328774170353	20.53946907935124	39.62656553892405	19.22067764002119
19	32.74096725396489	20.600570277852682	26.711455088228604	19.947007379953835
20	34.33055461529194	20.527420955703064	24.81655297445224	20.32547145455276
21	19.632226244638108	30.109886537273646	29.289371068821556	20.968516149266687
22	21.550746505566043	24.43330789840563	25.53657179247394	28.479373803554388
23	16.91470502177268	33.03958860438743	24.4695478893791	25.576158484460787
24	16.865843186977315	22.03821741317223	46.89904246103006	14.196896938820394
25	13.189635553821647	24.063162766325686	43.92229533967103	18.82490634018164
26	12.364147843863966	40.493934821563464	28.08723606481508	19.054681269757488
27	15.115040456834251	39.2907479972385	30.080053088240078	15.51415845768718
28	11.080640195217851	28.89302604880829	45.799603368119904	14.226730387853962
29	11.384042547088082	23.82220029336225	44.799800345379545	19.993956814170126
30	15.665716203578867	33.59361105214543	33.46901815441869	17.27165458985701
31	33.17967194680467	25.970973584010803	24.331759427656756	16.517595041527777
32	33.84528296835168	26.162213641918292	26.94668035945481	13.045823030275214
33	30.575460458249427	28.36204802802814	24.334054308351643	16.728437205370785
34	17.493684297087604	28.658374497755794	29.164586931036922	24.683354274119672
35	19.59808989430162	24.774862641828406	34.26983589690631	21.357211566963663
36	34.91613167260467	23.41858815114849	26.217195158566692	15.448085017680144
37	18.334853691793697	36.12964546005665	29.616391567843365	15.91910928030629
38	19.103160624437038	37.123615661030826	22.89153055155545	20.88169316297669
39	19.760357083436126	34.34900928088001	27.80247961859083	18.088154017093036
40	25.288246577281065	26.15093047850175	23.688619112913866	24.87220383130332
41	15.375413795675296	23.513156359783746	27.678364821008866	33.43306502353209
42	21.57034861150156	25.254014606915625	23.519084801578877	29.656551980003936
43	22.66223372212437	26.240143965515593	24.74856713386613	26.349055178493906
44	17.805979311650535	33.119718188650666	29.220524647974866	19.853777851723933
45	14.213439203829392	45.269199067513476	20.33780643828779	20.17955529036934
46	21.734623821244092	36.834078213358886	24.667768209400215	16.76352975599681
47	22.179639435994822	27.405178398288022	26.144715176619755	24.270466989097407
48	24.10925162028139	22.791225141182974	33.42034755968124	19.679175678854396
49	21.219996825415038	22.784627359185166	35.28608556462671	20.709290250773087
50	18.63194512175298	37.545299988716835	25.285186736354543	18.537568153175638
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1736.0
1	2323.0
2	2910.0
3	7530.5
4	12151.0
5	8914.5
6	5678.0
7	5944.0
8	6210.0
9	6456.0
10	6702.0
11	6630.5
12	6559.0
13	6182.0
14	5805.0
15	5373.0
16	4941.0
17	4624.0
18	4307.0
19	4227.0
20	4147.0
21	4116.0
22	4085.0
23	3965.5
24	3846.0
25	5048.5
26	6251.0
27	8606.0
28	10961.0
29	11888.5
30	12816.0
31	13956.0
32	15096.0
33	16831.0
34	18566.0
35	20421.0
36	22276.0
37	23500.0
38	24724.0
39	36800.0
40	48876.0
41	77195.5
42	105515.0
43	125481.0
44	145447.0
45	140136.5
46	134826.0
47	116687.5
48	98549.0
49	90480.5
50	82412.0
51	78898.0
52	75384.0
53	65384.5
54	55385.0
55	46146.0
56	36907.0
57	31325.5
58	25744.0
59	23025.5
60	20307.0
61	16784.0
62	13261.0
63	11112.5
64	8964.0
65	6902.5
66	4841.0
67	4177.5
68	3514.0
69	2896.5
70	2279.0
71	1924.5
72	1570.0
73	1293.5
74	1017.0
75	841.5
76	666.0
77	459.0
78	252.0
79	196.5
80	141.0
81	117.5
82	94.0
83	59.5
84	25.0
85	28.5
86	32.0
87	19.0
88	6.0
89	9.5
90	13.0
91	10.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	1.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1045806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.394093646008052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60694986204379	13.050837909114138
2	7.699826104436876	2.5246334042823104
3	2.635703660550459	1.2962991790257137
4	1.2861538118784943	0.8434130414042504
5	0.7867746485093552	0.644922863298372
6	0.520551383395757	0.5120380876169448
7	0.3627927568693504	0.4163360901146693
8	0.31311701100726475	0.41066156804889853
9	0.2536181310762221	0.37420554520702176
>10	3.172005055809794	12.22829058953702
>50	1.4368683855676507	17.52993051285619
>100	1.9162171010807934	48.073188467104
>500	0.005888804858883814	0.5480667672004174
>1k	0.002944402429441907	0.8498558660675134
>5k	5.888804858883815E-4	0.6973201091225321
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7223	0.6906634691328984	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3012	0.2880075272086792	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1881	0.1798612744619939	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1740	0.1663788503795159	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1095	0.10470393170435052	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1075	0.10279153112527563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03136336949682828	0.0
2	0.0	0.0	0.0	0.11818635578682853	0.0
3	0.0	0.0	0.0	0.15576502716565022	0.0
4	0.0	0.0	0.0	0.21361514468266582	0.0
5	0.0	0.0	0.0	0.32883727957192826	0.0
6	0.0	0.0	0.0	0.4350711317395387	0.0
7	0.0	0.0	0.0	0.497893490762149	0.0
8	0.0	0.0	0.0	0.6621687005046825	0.0
9	0.0	0.0	0.0	0.7376129033491872	0.0
10	0.0	0.0	0.0	0.8849633679669078	0.0
11	0.0	0.0	0.0	1.0445531962907078	0.0
12	0.0	0.0	0.0	1.1765088362468756	0.0
13	0.0	0.0	0.0	1.2294823322872501	0.0
14	0.0	0.0	0.0	1.2465026974410167	0.0
15	0.0	0.0	0.0	1.2799697075748275	0.0
16	0.0	0.0	0.0	1.3558920105641008	0.0
17	0.0	0.0	0.0	1.4391770557828125	0.0
18	0.0	0.0	0.0	1.5628137532200044	0.0
19	0.0	0.0	0.0	1.6112931078995532	0.0
20	0.0	0.0	0.0	1.6608242828975928	0.0
21	0.0	0.0	0.0	1.729670703744289	0.0
22	0.0	0.0	0.0	1.793831743172252	0.0
23	0.0	0.0	0.0	1.876925548333056	0.0
24	0.0	0.0	0.0	1.933915085589488	0.0
25	0.0	0.0	0.0	1.975127318068552	0.0
26	0.0	0.0	0.0	2.0172001308081997	0.0
27	0.0	0.0	0.0	2.058890463432032	0.0
28	0.0	0.0	0.0	2.1086128784879796	0.0
29	0.0	0.0	0.0	2.159387113862418	0.0
30	0.0	0.0	0.0	2.2268948543037617	0.0
31	0.0	0.0	0.0	2.299183596192793	0.0
32	0.0	0.0	0.0	2.35655561356504	0.0
33	0.0	0.0	0.0	2.406564888707848	0.0
34	0.0	0.0	0.0	2.4645106262538174	0.0
35	0.0	0.0	0.0	2.5602262752365164	0.0
36	0.0	0.0	0.0	2.6241004545776176	0.0
37	0.0	0.0	0.0	2.688070253947673	0.0
38	0.0	0.0	0.0	2.7406612698722324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.856154E-4	44.000004	35
CTTCGAA	20	7.856154E-4	44.000004	41
AGCGTGC	20	7.856154E-4	44.000004	26
GCCCATA	20	7.856154E-4	44.000004	11
GTACCGT	20	7.856154E-4	44.000004	27
GTCGAAT	20	7.856154E-4	44.000004	43
ATAGCCG	40	8.310963E-9	44.000004	1
ATGCTCG	20	7.856154E-4	44.000004	1
CGTCTAT	20	7.856154E-4	44.000004	41
ATTCGAC	20	7.856154E-4	44.000004	15
CGTCTAA	20	7.856154E-4	44.000004	32
ATGTCGA	20	7.856154E-4	44.000004	41
TCGATAG	65	0.0	44.000004	1
TAGCCGT	20	7.856154E-4	44.000004	44
ACCTCGC	20	7.856154E-4	44.000004	9
CGTAGAT	20	7.856154E-4	44.000004	11
TAACACG	20	7.856154E-4	44.000004	1
TCGTCAC	20	7.856154E-4	44.000004	32
CGTACAT	20	7.856154E-4	44.000004	35
TCGTACA	20	7.856154E-4	44.000004	34
>>END_MODULE