FastQCFastQC Report
Thu 26 May 2016
SRR1545914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545914_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2020000
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305441.512079207920792No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA22450.11113861386138613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.8578986E-444.00000433
CCACGTA207.8578986E-444.00000437
TACCGTA207.8578986E-444.00000438
CGTTTTT141250.042.146551
CGTAAGG2750.040.02
CGGTCTA2750.040.031
TCACGAC3050.039.6721325
CTATGCG800.038.5000041
GTTATCG404.126614E-738.5000041
CATACGA9750.037.90769218
CGTTCGA1050.037.71428714
ATACGAA9800.037.71428719
CGACCCG6050.037.45454432
CGGGATA3300.037.3333326
TATAGCG1300.037.2307661
TCGACAC651.0913936E-1137.23076634
ACGGGCT4200.037.1904755
GGGCGAT30950.036.962847
GCTACGA3100.036.90322510
TACGGGA5200.036.807694
Produced by FastQC (version 0.11.5)