FastQCFastQC Report
Thu 26 May 2016
SRR1545913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545913_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1854150
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149100.8041420597039075No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32170.1735026831701858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA254.4439E-544.015
CGTTACG254.4439E-544.023
ATCCGGT207.8577327E-444.012
CTAACGC254.4439E-544.032
CTTACGT302.5285608E-644.011
CGTTTTT80650.041.5449451
CGTTAGG2650.041.5094342
CGGGATA3200.039.8756
TCGATAG1050.039.8095251
ACCGAAC501.3496901E-939.611
TCACGAC2200.039.025
ACGTTAG850.038.8235321
CGGTCTA2300.038.2608731
CGACAGG1500.038.133332
ACGGGAT6700.037.7611965
TAGCATA9850.037.74619330
ACTACGG700.037.7142872
AACTATC357.292625E-637.71428712
CATATGC9850.037.52284633
CGCGACC4700.037.4468110
Produced by FastQC (version 0.11.5)