##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545913_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1854150
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.670151821589407	31.0	31.0	33.0	30.0	34.0
2	32.10438475851468	33.0	31.0	34.0	30.0	34.0
3	32.1527605641399	33.0	31.0	34.0	30.0	34.0
4	35.86298573470323	37.0	35.0	37.0	35.0	37.0
5	35.853212523258634	37.0	35.0	37.0	35.0	37.0
6	35.90659116036998	37.0	35.0	37.0	35.0	37.0
7	36.08677722945824	37.0	35.0	37.0	35.0	37.0
8	36.03425666747566	37.0	35.0	37.0	35.0	37.0
9	37.819100935738746	39.0	38.0	39.0	35.0	39.0
10	37.57515896772106	39.0	37.0	39.0	35.0	39.0
11	37.2968573200658	39.0	37.0	39.0	34.0	39.0
12	36.62964215408678	39.0	35.0	39.0	33.0	39.0
13	36.42982282986814	39.0	35.0	39.0	33.0	39.0
14	37.450381576463606	40.0	35.0	41.0	33.0	41.0
15	37.62480004314646	40.0	35.0	41.0	33.0	41.0
16	37.69760591106437	40.0	35.0	41.0	33.0	41.0
17	37.63114365072944	39.0	35.0	41.0	33.0	41.0
18	37.55324542243076	39.0	36.0	41.0	33.0	41.0
19	37.4608904349702	39.0	35.0	41.0	33.0	41.0
20	37.29842137906858	39.0	35.0	41.0	33.0	41.0
21	37.180944907369955	39.0	35.0	41.0	33.0	41.0
22	37.174408758730415	39.0	35.0	41.0	33.0	41.0
23	37.14934552220694	39.0	35.0	41.0	33.0	41.0
24	37.09268883315805	39.0	35.0	41.0	33.0	41.0
25	36.995976053717335	38.0	35.0	41.0	33.0	41.0
26	36.89862956071515	38.0	35.0	41.0	33.0	41.0
27	36.80270905805895	38.0	35.0	41.0	32.0	41.0
28	36.763414502602274	38.0	35.0	41.0	32.0	41.0
29	36.696746217943534	38.0	35.0	41.0	32.0	41.0
30	36.59017986678532	38.0	35.0	41.0	31.0	41.0
31	36.40529514872044	38.0	35.0	41.0	31.0	41.0
32	36.223485694253434	38.0	35.0	41.0	31.0	41.0
33	36.04449532130626	38.0	35.0	41.0	30.0	41.0
34	35.86504166329585	38.0	35.0	41.0	30.0	41.0
35	35.637694361297626	38.0	35.0	41.0	29.0	41.0
36	35.48584958067039	38.0	35.0	41.0	27.0	41.0
37	35.412523258636035	38.0	35.0	40.0	27.0	41.0
38	35.321872016827115	38.0	35.0	40.0	27.0	41.0
39	35.2585804816223	38.0	35.0	40.0	27.0	41.0
40	35.097805463419895	38.0	34.0	40.0	25.0	41.0
41	34.973183938732035	37.0	34.0	40.0	25.0	41.0
42	34.96110670657714	37.0	34.0	40.0	25.0	41.0
43	34.89086427743171	37.0	34.0	40.0	25.0	41.0
44	34.75936898309198	37.0	34.0	40.0	24.0	41.0
45	34.656442035434026	37.0	34.0	40.0	24.0	41.0
46	34.62518620392093	37.0	34.0	40.0	24.0	41.0
47	34.54668446457946	36.0	34.0	40.0	24.0	41.0
48	34.48397864250465	36.0	34.0	40.0	23.0	41.0
49	34.42056575789445	36.0	34.0	40.0	24.0	41.0
50	34.270360003235986	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	3.0
12	4.0
13	12.0
14	29.0
15	54.0
16	155.0
17	301.0
18	736.0
19	1406.0
20	2464.0
21	3930.0
22	6115.0
23	9007.0
24	13165.0
25	19577.0
26	26924.0
27	32740.0
28	34286.0
29	35262.0
30	38642.0
31	45453.0
32	55750.0
33	74732.0
34	140574.0
35	274502.0
36	111336.0
37	145012.0
38	245945.0
39	536032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.640590027775527	23.592319930965672	29.39228217781733	15.37480786344147
2	33.017824879324756	25.02834182779171	29.160855378475315	12.792977914408219
3	28.710298519537254	23.980961626621365	33.3885607960521	13.920179057789284
4	25.94223768303535	27.08497155030607	32.524229431275785	14.44856133538279
5	22.585443464660358	31.417846452552382	31.7741283067713	14.222581776015964
6	21.17994768492301	39.791980152630586	28.870695466925543	10.15737669552086
7	85.8440794973438	3.946929860043686	8.46733004341612	1.7416605991963974
8	86.63446862443708	3.541784645255238	8.210554701615296	1.6131920286923926
9	82.33271310303913	5.00666073402907	9.877356200954615	2.7832699619771866
10	48.223390772051886	26.562198311894935	15.460237844834559	9.754173071218618
11	39.0645848502009	21.76377315751153	26.047892565326432	13.12374942696114
12	36.378556211741234	21.639996764015855	27.58104791953186	14.400399104711054
13	21.719871639295636	35.47782002534854	27.440983739179682	15.361324596176146
14	16.803117331391743	35.73502683170186	31.16085537847531	16.301000458431087
15	15.559582558045465	23.204756896691205	44.79810155596905	16.437558989294285
16	18.080414205970392	19.806434215139014	43.11727745867378	18.99587412021681
17	18.177277998004477	19.55936682576922	31.982633551762262	30.28072162446404
18	21.5692905104765	22.255534881212416	35.83561200550117	20.339562602809913
19	27.73071218617695	23.489199902920475	28.40439015182159	20.37569775908098
20	28.507456246797723	23.358951541137447	28.773508076477093	19.360084135587734
21	22.47849418871181	26.667421729633524	31.141817005096673	19.71226707655799
22	22.93244883100073	23.815279238465063	28.72761103470593	24.524660895828276
23	19.386133807944343	28.20877491033627	29.74554378016881	22.659547501550577
24	19.08993339265971	23.23652347436831	39.991478575088315	17.682064557883667
25	18.529245206698487	25.872825823153466	35.820672545371195	19.77725642477685
26	17.91041717228919	31.25879783189062	29.73146724914381	21.099317746676373
27	17.946390529353074	30.589164846425586	31.46994579726559	19.99449882695575
28	16.20952997330313	26.772267615888683	38.331904107003204	18.68629830380498
29	16.7172019523771	24.57390178788124	37.28630369711188	21.422592562629777
30	19.30911738532481	29.001159560984817	32.92813418547583	18.761588868214545
31	26.927918453199577	25.806973545829624	28.429253296658846	18.83585470431195
32	27.778928349917752	25.15249575277081	28.80139147318178	18.267184424129653
33	25.733354906560958	26.112666181269045	27.969527815980367	20.18445109618963
34	19.53423401558666	25.922821778173287	31.134482107704336	23.40846209853572
35	20.56640509128172	25.355499824717526	32.593317692743305	21.484777391257452
36	27.33516705768142	26.206024323814148	27.277836205269264	19.180972413235175
37	19.637731575115282	30.68424884718065	30.2533236253809	19.42469595232317
38	21.19618153871046	30.919882425909446	27.56195561308416	20.32198042229593
39	20.687808429738695	30.159480085214245	28.38184612895397	20.770865356093086
40	23.670954345657037	26.001833724348085	27.684976943612977	22.6422349863819
41	18.529892403527224	24.724968314321927	30.006256236011108	26.73888304613974
42	21.425882479842514	25.389154059811776	27.039398106949275	26.14556535339643
43	21.168837472696385	26.31502305638702	28.467114311139873	24.049025159776715
44	19.6117897688968	28.391931612868433	29.745004449478195	22.251274168756574
45	18.181646576598443	34.18040611601003	26.43135668635224	21.20659062103929
46	20.96745139282151	31.111668419491412	27.651268775449665	20.269611412237413
47	20.543968934552222	27.83749966291832	29.172936385945043	22.44559501658442
48	21.383329288353153	26.015316991613407	32.12064827549012	20.48070544454332
49	20.829274869886472	26.016125987649325	32.23665830704096	20.91794083542324
50	19.724024485613352	31.204595097484024	28.9762424830785	20.095137933824127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2094.0
1	3664.5
2	5235.0
3	16006.5
4	26778.0
5	18844.5
6	10911.0
7	10838.5
8	10766.0
9	11169.0
10	11572.0
11	10942.5
12	10313.0
13	9790.0
14	9267.0
15	8693.0
16	8119.0
17	8116.0
18	8113.0
19	7730.5
20	7348.0
21	7910.0
22	8472.0
23	8630.5
24	8789.0
25	11219.5
26	13650.0
27	17165.0
28	20680.0
29	24215.5
30	27751.0
31	32971.0
32	38191.0
33	42836.5
34	47482.0
35	52688.5
36	57895.0
37	64416.5
38	70938.0
39	82202.5
40	93467.0
41	116158.5
42	138850.0
43	153258.0
44	167666.0
45	173297.5
46	178929.0
47	169781.0
48	160633.0
49	152629.5
50	144626.0
51	136290.5
52	127955.0
53	116595.5
54	105236.0
55	95104.0
56	84972.0
57	76424.0
58	67876.0
59	61944.0
60	56012.0
61	48917.0
62	41822.0
63	35258.5
64	28695.0
65	23324.5
66	17954.0
67	14965.5
68	11977.0
69	10194.0
70	8411.0
71	7113.0
72	5815.0
73	4852.5
74	3890.0
75	3046.5
76	2203.0
77	1772.0
78	1341.0
79	1050.5
80	760.0
81	567.5
82	375.0
83	279.5
84	184.0
85	130.0
86	76.0
87	53.5
88	31.0
89	23.5
90	16.0
91	13.5
92	11.0
93	6.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1854150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.390902610158307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6152950412877	13.498000371217373
2	6.507756642487232	2.263515239602125
3	2.4006427886312847	1.2524803481659657
4	1.3873450540297423	0.9650873088526425
5	0.978122796902447	0.8505219150853054
6	0.7487622645788361	0.7812990972871275
7	0.6030425993948528	0.7341218581096817
8	0.5122361950719456	0.7126599825515407
9	0.4395447032827919	0.6879671214841766
>10	5.68291570233284	22.9294475131941
>50	1.899426827157264	23.895914609878353
>100	1.221461584629887	30.034415732677417
>500	0.001880618298102859	0.21945490928025468
>1k	0.0012537455320685725	0.36237859833460834
>5k	0.0	0.0
>10k+	3.134363830171431E-4	0.8127353942793337
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14910	0.8041420597039075	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3217	0.1735026831701858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012188873607852655	0.0
2	0.0	0.0	0.0	0.04837796294798156	0.0
3	0.0	0.0	0.0	0.0755602297548742	0.0
4	0.0	0.0	0.0	0.12409999191003965	0.0
5	0.0	0.0	0.0	0.23762910228406547	0.0
6	0.0	0.0	0.0	0.3664212712024378	0.0
7	0.0	0.0	0.0	0.45039505973087396	0.0
8	0.0	0.0	0.0	0.6613272928295985	0.0
9	0.0	0.0	0.0	0.7586764824852358	0.0
10	0.0	0.0	0.0	0.9121699970336812	0.0
11	0.0	0.0	0.0	1.0345980638028207	0.0
12	0.0	0.0	0.0	1.1486125717983982	0.0
13	0.0	0.0	0.0	1.1896556373540437	0.0
14	0.0	0.0	0.0	1.2056737588652482	0.0
15	0.0	0.0	0.0	1.2270851872825823	0.0
16	0.0	0.0	0.0	1.278968799719548	0.0
17	0.0	0.0	0.0	1.3385109079632176	0.0
18	0.0	0.0	0.0	1.4339724402017098	0.0
19	0.0	0.0	0.0	1.4763638324838875	0.0
20	0.0	0.0	0.0	1.5278159803683629	0.0
21	5.393306906129493E-5	0.0	0.0	1.5942075883828168	0.0
22	5.393306906129493E-5	0.0	0.0	1.6603834641210258	0.0
23	5.393306906129493E-5	0.0	0.0	1.7379931505002293	0.0
24	5.393306906129493E-5	0.0	0.0	1.8001779791279022	0.0
25	5.393306906129493E-5	0.0	0.0	1.8527627214626647	0.0
26	5.393306906129493E-5	0.0	0.0	1.904754200037753	0.0
27	5.393306906129493E-5	0.0	0.0	1.9587412021681094	0.0
28	5.393306906129493E-5	0.0	0.0	2.0125124720222205	0.0
29	5.393306906129493E-5	0.0	0.0	2.0849985168406007	0.0
30	1.0786613812258986E-4	0.0	0.0	2.1798667853194185	0.0
31	1.0786613812258986E-4	0.0	0.0	2.2563438772483346	0.0
32	1.0786613812258986E-4	0.0	0.0	2.3270501307876925	0.0
33	1.0786613812258986E-4	0.0	0.0	2.4020710298519536	0.0
34	1.0786613812258986E-4	0.0	0.0	2.485990885311329	0.0
35	1.0786613812258986E-4	0.0	0.0	2.5897581101852603	0.0
36	1.0786613812258986E-4	0.0	0.0	2.6694172531887927	0.0
37	1.0786613812258986E-4	0.0	0.0	2.7580832187255617	0.0
38	1.0786613812258986E-4	0.0	0.0	2.859477388560796	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	25	4.4439E-5	44.0	15
CGTTACG	25	4.4439E-5	44.0	23
ATCCGGT	20	7.8577327E-4	44.0	12
CTAACGC	25	4.4439E-5	44.0	32
CTTACGT	30	2.5285608E-6	44.0	11
CGTTTTT	8065	0.0	41.544945	1
CGTTAGG	265	0.0	41.509434	2
CGGGATA	320	0.0	39.875	6
TCGATAG	105	0.0	39.809525	1
ACCGAAC	50	1.3496901E-9	39.6	11
TCACGAC	220	0.0	39.0	25
ACGTTAG	85	0.0	38.823532	1
CGGTCTA	230	0.0	38.26087	31
CGACAGG	150	0.0	38.13333	2
ACGGGAT	670	0.0	37.761196	5
TAGCATA	985	0.0	37.746193	30
ACTACGG	70	0.0	37.714287	2
AACTATC	35	7.292625E-6	37.714287	12
CATATGC	985	0.0	37.522846	33
CGCGACC	470	0.0	37.44681	10
>>END_MODULE