FastQCFastQC Report
Thu 26 May 2016
SRR1545912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545912_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1643981
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97480.5929508917682138No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39580.24075704037942047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC254.4436798E-544.037
ACCGGAC254.4436798E-544.019
TCCGCGA254.4436798E-544.030
GTCGATA207.857472E-444.036
TAGACGC302.5283844E-644.037
GCGAACG408.3164196E-944.01
AGGCGTA254.4436798E-544.040
ATGTCGC207.857472E-444.036
CTACGCT207.857472E-444.033
CGTAGCG254.4436798E-544.013
CACTACG207.857472E-444.020
TTGCGTC302.5283844E-644.016
ACTAACG207.857472E-444.01
ATGCGCT207.857472E-444.040
CCGGTTA207.857472E-444.042
CGCACCG207.857472E-444.033
CGCCGTT254.4436798E-544.026
TGTCGAT207.857472E-444.035
GTCGGAC207.857472E-444.034
GCTTGCG1050.041.904761
Produced by FastQC (version 0.11.5)