FastQCFastQC Report
Thu 26 May 2016
SRR1545910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545910_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3021461
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167240.5535070616499765No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA62660.20738311697552939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG551.8189894E-1244.0000041
ACTACCG207.8585197E-444.0000041
AACGATC207.8585197E-444.00000442
TGTCGTA302.529092E-644.00000422
GCGAAAA254.444568E-544.042
CCGTCGA254.444568E-544.039
TATCGTG351.4471334E-744.01
TACCGTT254.444568E-544.034
TATAGCG1850.042.8108141
GTTAGCG1600.042.6250041
CGACGGT4950.042.2222228
CGTTTTT99050.041.4235231
CGGTCTA5000.041.3631
CGGGCTA1600.041.2500046
CCCGTAA700.040.85714327
TAATGCG1400.040.8571431
ACGGGAT7350.040.707485
CGACTAT603.6379788E-1240.33333623
TGCGTAG1200.040.3333361
CGTATGG2300.040.1739162
Produced by FastQC (version 0.11.5)