##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545910_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3021461
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714143919117276	31.0	31.0	33.0	30.0	34.0
2	32.137246517496	33.0	31.0	34.0	30.0	34.0
3	32.20873080936673	33.0	31.0	34.0	30.0	34.0
4	35.89002638127714	37.0	35.0	37.0	35.0	37.0
5	35.85806436025486	37.0	35.0	37.0	35.0	37.0
6	35.92077442005705	37.0	35.0	37.0	35.0	37.0
7	36.11801542366425	37.0	35.0	37.0	35.0	37.0
8	36.067329017319764	37.0	35.0	37.0	35.0	37.0
9	37.859489829589066	39.0	38.0	39.0	35.0	39.0
10	37.58293587109018	39.0	37.0	39.0	35.0	39.0
11	37.32675020461955	39.0	37.0	39.0	35.0	39.0
12	36.50663536613579	38.0	35.0	39.0	33.0	39.0
13	36.25048809168809	38.0	35.0	39.0	33.0	39.0
14	37.256047984733215	39.0	35.0	41.0	33.0	41.0
15	37.45124428215357	39.0	35.0	41.0	33.0	41.0
16	37.53287664477549	39.0	35.0	41.0	33.0	41.0
17	37.48072439127958	39.0	35.0	41.0	33.0	41.0
18	37.43051490653032	39.0	35.0	41.0	33.0	41.0
19	37.34785555729496	38.0	35.0	41.0	33.0	41.0
20	37.20812745886841	38.0	35.0	41.0	33.0	41.0
21	37.07656329173204	38.0	35.0	41.0	33.0	41.0
22	37.041713594846996	38.0	35.0	41.0	33.0	41.0
23	37.03446246699858	38.0	35.0	41.0	33.0	41.0
24	36.979690288903285	38.0	35.0	41.0	33.0	41.0
25	36.87837705004301	38.0	35.0	41.0	33.0	41.0
26	36.77418176173712	38.0	35.0	41.0	32.0	41.0
27	36.69433661397582	38.0	35.0	41.0	32.0	41.0
28	36.672913534214075	38.0	35.0	41.0	32.0	41.0
29	36.642159538051295	38.0	35.0	41.0	32.0	41.0
30	36.54617749492712	38.0	35.0	41.0	32.0	41.0
31	36.378022751245176	38.0	35.0	41.0	31.0	41.0
32	36.20971642526579	38.0	35.0	41.0	31.0	41.0
33	36.05197750359842	38.0	35.0	41.0	30.0	41.0
34	35.91489150447416	38.0	35.0	41.0	30.0	41.0
35	35.72374225581598	38.0	35.0	41.0	30.0	41.0
36	35.588206169134736	38.0	35.0	40.0	29.0	41.0
37	35.5245376326221	38.0	35.0	40.0	29.0	41.0
38	35.46703697317291	37.0	35.0	40.0	29.0	41.0
39	35.435334760236856	37.0	35.0	40.0	29.0	41.0
40	35.28318551852895	37.0	35.0	40.0	28.0	41.0
41	35.18782734577742	37.0	34.0	40.0	27.0	41.0
42	35.16103666405093	37.0	34.0	40.0	27.0	41.0
43	35.078424642912815	37.0	34.0	40.0	27.0	41.0
44	34.93471999142137	36.0	34.0	40.0	27.0	41.0
45	34.833468974115505	36.0	34.0	40.0	26.0	41.0
46	34.80743554194478	36.0	34.0	40.0	26.0	41.0
47	34.74523384548071	36.0	34.0	40.0	26.0	41.0
48	34.706956336686126	36.0	34.0	40.0	26.0	41.0
49	34.66302791927482	36.0	34.0	40.0	26.0	41.0
50	34.45585066297397	35.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	4.0
13	4.0
14	28.0
15	75.0
16	136.0
17	422.0
18	952.0
19	1943.0
20	3523.0
21	5719.0
22	8977.0
23	12994.0
24	19109.0
25	27669.0
26	38849.0
27	47845.0
28	52331.0
29	54947.0
30	62285.0
31	73410.0
32	91539.0
33	122907.0
34	243093.0
35	530736.0
36	171203.0
37	219321.0
38	377287.0
39	854145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.37724332698651	23.193978012623695	29.063886642918774	13.36489201747102
2	32.46297072839928	24.929628414862876	29.83914073357227	12.76826012316558
3	29.18617847458564	24.66157928234056	33.087006583900965	13.06523565917283
4	26.596504141539473	26.808388392238058	31.708931540072832	14.886175926149633
5	23.05331758377818	31.195338943643492	30.5526697183912	15.19867375418713
6	20.737749055837558	40.98020791928143	27.898225394933114	10.383817629947895
7	86.78801414282692	4.2022054893311545	7.294451260499474	1.7153291073424413
8	87.95526402624427	3.2251285057129646	6.92092335462877	1.8986841134140076
9	83.89375206233011	4.488159867031214	8.82437337433778	2.793714696300895
10	51.34770893948325	23.019757660284213	14.300002548436005	11.33253085179653
11	44.10472284765549	20.951056459110344	22.009584105172962	12.934636588061208
12	39.014834214308905	22.15362038431077	25.601389526457563	13.230155874922762
13	21.058388640462347	38.481383674983725	25.68644771519474	14.773779969359193
14	15.082372401960509	39.69109645962665	30.274724710992462	14.951806427420378
15	14.334290596502818	22.545748563360572	47.80515121658032	15.314809623556286
16	15.911805580148147	18.8049423772142	46.60381186452514	18.679440178112507
17	17.346674340658378	18.19411205373824	30.532050554351027	33.92716305125236
18	21.40189795598884	22.608863725197843	35.40780437013749	20.581433948675823
19	28.58021334711916	23.354926639794456	27.166294716364035	20.898565296722346
20	30.112088158675554	22.409953330524537	26.419404387480093	21.058554123319812
21	22.524732240462477	28.08373167815173	28.46080091717219	20.930735164213605
22	22.749325574614403	24.472134507114273	26.821726310549764	25.956813607721564
23	19.821371184337643	29.007589374809072	27.14276305403247	24.028276386820814
24	19.651221710291807	22.85543318282116	40.03917310201919	17.454172004867843
25	17.82859351816886	24.160397900221117	37.11333689231799	20.897671689292036
26	16.200043621281228	33.54857136994321	29.070903116075304	21.180481892700254
27	17.395524880182137	32.50679058905609	30.292497569884237	19.805186960877535
28	15.314644140698821	27.142729957460975	39.49893776553793	18.043688136302272
29	16.617159711808295	23.664842935255496	38.02299615980481	21.695001193131404
30	18.98842976957174	29.33779386859536	32.82517960681935	18.848596755013553
31	28.47629011263094	26.284800631217813	26.52167279339366	18.717236462757587
32	29.668263134953587	25.66665596544188	27.81895910620723	16.8461217933973
33	27.379966181923248	25.609465089901875	27.175793432382545	19.834775295792333
34	19.63252214739823	27.233083597637037	29.79495681062903	23.339437444335704
35	20.288992642963123	24.886966934208317	32.73492525635777	22.08911516647079
36	28.424527074815792	25.38679135689655	27.689551511669354	18.499130056618306
37	20.116096153483365	32.48428492044081	28.857728099088487	18.54189082698734
38	20.64570749051535	32.6618149299296	25.47036019991653	21.222117379638526
39	21.14500236805969	30.590532196179264	27.508744941602753	20.75572049415829
40	23.457426721708472	26.258323374023362	26.90364032499509	23.380609579273074
41	17.343728745795495	24.440097025908987	29.02622274455967	29.18995148373585
42	21.94362263818729	25.3121254916082	25.425348862685965	27.318903007518546
43	21.9664592725175	26.273415410624196	27.178010902672582	24.58211441418572
44	20.335394036196398	29.437017389931558	28.798716912116358	21.428871661755686
45	17.55528203077915	35.657518002052655	25.046260732804427	21.740939234363772
46	21.163437158381328	32.17383907983588	26.96136074567899	19.701363016103798
47	20.813573301128162	28.094289484458017	28.22422662413978	22.867910590274043
48	21.815340327080175	24.549448098122067	31.696288649762483	21.93892292503527
49	20.34244360592442	24.889118211355367	33.655870454723726	21.11256772799649
50	19.55269321695696	32.63864732988445	28.274930571667152	19.533728881491438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2254.0
1	4840.0
2	7426.0
3	19838.5
4	32251.0
5	23046.5
6	13842.0
7	13913.0
8	13984.0
9	14006.5
10	14029.0
11	13347.0
12	12665.0
13	11993.0
14	11321.0
15	10525.5
16	9730.0
17	9855.5
18	9981.0
19	10099.0
20	10217.0
21	11966.0
22	13715.0
23	14793.5
24	15872.0
25	18415.0
26	20958.0
27	24752.0
28	28546.0
29	32966.0
30	37386.0
31	44222.5
32	51059.0
33	58635.5
34	66212.0
35	75738.0
36	85264.0
37	94669.5
38	104075.0
39	127228.5
40	150382.0
41	195037.0
42	239692.0
43	277755.5
44	315819.0
45	317125.0
46	318431.0
47	295070.0
48	271709.0
49	260302.5
50	248896.0
51	231404.5
52	213913.0
53	197963.5
54	182014.0
55	165150.5
56	148287.0
57	130852.0
58	113417.0
59	101224.5
60	89032.0
61	78412.5
62	67793.0
63	56516.5
64	45240.0
65	34029.5
66	22819.0
67	17419.0
68	12019.0
69	10065.0
70	8111.0
71	6815.0
72	5519.0
73	4600.0
74	3681.0
75	2656.5
76	1632.0
77	1334.0
78	1036.0
79	818.5
80	601.0
81	432.0
82	263.0
83	203.0
84	143.0
85	133.0
86	123.0
87	80.5
88	38.0
89	31.5
90	25.0
91	24.5
92	24.0
93	16.0
94	8.0
95	6.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3021461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.892617555193986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9100679493216	11.1016001282949
2	7.506344728489049	2.0856555310068963
3	2.498760913808781	1.0414298921223732
4	1.275236934303317	0.7086551608213609
5	0.7656078517713699	0.5318148540956645
6	0.49260613513866525	0.41061531844942095
7	0.40927557252099006	0.39801363026020237
8	0.3252593878653296	0.36149634254796226
9	0.2713029143930827	0.3392196868145369
>10	3.384920047033967	11.237297533466146
>50	1.093488212467411	10.956962670450585
>100	2.040377416566271	57.64353364851004
>500	0.024823868837652446	2.1713658379837812
>1k	0.0014460506119020843	0.24257984450806463
>5k	2.4100843531701404E-4	0.2098005942977961
>10k+	2.4100843531701404E-4	0.5599593263703068
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16724	0.5535070616499765	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6266	0.20738311697552939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02528578062069972	0.0
2	0.0	0.0	0.0	0.08949312931724089	0.0
3	0.0	0.0	0.0	0.12596555110259572	0.0
4	0.0	0.0	0.0	0.18702872550729596	0.0
5	0.0	0.0	0.0	0.32725889892340165	0.0
6	3.3096571493062464E-5	0.0	0.0	0.47245355806346667	0.0
7	3.3096571493062464E-5	0.0	0.0	0.5734973908317863	0.0
8	3.3096571493062464E-5	0.0	0.0	0.8533950959486156	0.0
9	3.3096571493062464E-5	0.0	0.0	0.9673465915992296	0.0
10	3.3096571493062464E-5	0.0	0.0	1.1500396662409345	0.0
11	3.3096571493062464E-5	0.0	0.0	1.2966243813837082	0.0
12	3.3096571493062464E-5	0.0	0.0	1.4379136450875918	0.0
13	3.3096571493062464E-5	0.0	0.0	1.4896104897597553	0.0
14	3.3096571493062464E-5	0.0	0.0	1.5071847692225715	0.0
15	3.3096571493062464E-5	0.0	0.0	1.5359456898500428	0.0
16	3.3096571493062464E-5	0.0	0.0	1.605448489985474	0.0
17	3.3096571493062464E-5	0.0	0.0	1.689215912434415	0.0
18	3.3096571493062464E-5	0.0	0.0	1.8197818869745463	0.0
19	3.3096571493062464E-5	0.0	0.0	1.8777008870874057	0.0
20	3.3096571493062464E-5	0.0	0.0	1.9383999992056822	0.0
21	3.3096571493062464E-5	0.0	0.0	2.0201816273650395	0.0
22	3.3096571493062464E-5	0.0	0.0	2.103485697813078	0.0
23	3.3096571493062464E-5	0.0	0.0	2.201484646004036	0.0
24	3.3096571493062464E-5	0.0	0.0	2.2750583244331137	0.0
25	3.3096571493062464E-5	0.0	0.0	2.3367834302676753	0.0
26	3.3096571493062464E-5	0.0	0.0	2.4016858069655704	0.0
27	3.3096571493062464E-5	0.0	0.0	2.4609286699381525	0.0
28	3.3096571493062464E-5	0.0	0.0	2.5281809032120552	0.0
29	3.3096571493062464E-5	0.0	0.0	2.6010595536397787	0.0
30	3.3096571493062464E-5	0.0	0.0	2.69823108754341	0.0
31	3.3096571493062464E-5	0.0	0.0	2.794542110588222	0.0
32	3.3096571493062464E-5	0.0	0.0	2.8727162124548355	0.0
33	3.3096571493062464E-5	0.0	0.0	2.9573110491911034	0.0
34	3.3096571493062464E-5	0.0	0.0	3.0450500602192117	0.0
35	3.3096571493062464E-5	0.0	0.0	3.1586705901548955	0.0
36	3.3096571493062464E-5	0.0	0.0	3.256007606915992	0.0
37	6.619314298612493E-5	0.0	0.0	3.348247751667157	0.0
38	6.619314298612493E-5	0.0	0.0	3.441911048992524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	44.000004	1
ACTACCG	20	7.8585197E-4	44.000004	1
AACGATC	20	7.8585197E-4	44.000004	42
TGTCGTA	30	2.529092E-6	44.000004	22
GCGAAAA	25	4.444568E-5	44.0	42
CCGTCGA	25	4.444568E-5	44.0	39
TATCGTG	35	1.4471334E-7	44.0	1
TACCGTT	25	4.444568E-5	44.0	34
TATAGCG	185	0.0	42.810814	1
GTTAGCG	160	0.0	42.625004	1
CGACGGT	495	0.0	42.22222	28
CGTTTTT	9905	0.0	41.423523	1
CGGTCTA	500	0.0	41.36	31
CGGGCTA	160	0.0	41.250004	6
CCCGTAA	70	0.0	40.857143	27
TAATGCG	140	0.0	40.857143	1
ACGGGAT	735	0.0	40.70748	5
CGACTAT	60	3.6379788E-12	40.333336	23
TGCGTAG	120	0.0	40.333336	1
CGTATGG	230	0.0	40.173916	2
>>END_MODULE