FastQCFastQC Report
Thu 26 May 2016
SRR1545909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545909_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3251243
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185820.5715352559005894No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53460.16442941976345662No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA41370.12724364189326973No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33220.10217630610815616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGA207.8586076E-444.00000420
ATACGTC302.5291502E-644.00000415
ACCGTAA254.4446424E-544.018
CGGTCTA3850.043.4285731
CGACGGT3850.042.28571328
CGCATCG950.041.6842121
CGTTTTT104850.040.978541
ACGGGAT13350.040.374535
GCGATAC603.6379788E-1240.3333369
CTCGTAG1750.040.228571
ACGTATA557.8216544E-1140.013
CAATACG557.8216544E-1140.01
ACGCCGA1050.039.8095212
TAGGACG1000.039.6000021
ACGACTT501.3496901E-939.60000213
TATTGCG2500.039.61
CCGACGG950.039.368422
TACGGGT2350.039.3191534
CGGGTAT2800.039.2857136
GAGTACG1400.039.2857131
Produced by FastQC (version 0.11.5)