##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545909_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3251243
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73905057235033	31.0	31.0	33.0	30.0	34.0
2	32.16460473732661	33.0	31.0	34.0	30.0	34.0
3	32.2465564708636	34.0	31.0	34.0	30.0	34.0
4	35.91509155113906	37.0	35.0	37.0	35.0	37.0
5	35.89258938812017	37.0	35.0	37.0	35.0	37.0
6	35.94543133195519	37.0	35.0	37.0	35.0	37.0
7	36.133602440666536	37.0	35.0	37.0	35.0	37.0
8	36.08353143705346	37.0	35.0	37.0	35.0	37.0
9	37.83537496274502	39.0	38.0	39.0	35.0	39.0
10	37.586465238064335	39.0	37.0	39.0	35.0	39.0
11	37.313792601783376	39.0	37.0	39.0	34.0	39.0
12	36.64234724995948	39.0	35.0	39.0	33.0	39.0
13	36.43762923903258	39.0	35.0	39.0	33.0	39.0
14	37.50563215361017	40.0	35.0	41.0	33.0	41.0
15	37.678506035999156	40.0	35.0	41.0	33.0	41.0
16	37.74428303267396	40.0	35.0	41.0	33.0	41.0
17	37.669011513442705	39.0	35.0	41.0	33.0	41.0
18	37.63323873361665	39.0	35.0	41.0	33.0	41.0
19	37.56328210472118	39.0	36.0	41.0	34.0	41.0
20	37.42051055550139	39.0	35.0	41.0	33.0	41.0
21	37.31092262251699	39.0	35.0	41.0	33.0	41.0
22	37.27847749306957	39.0	35.0	41.0	33.0	41.0
23	37.25527498252207	39.0	35.0	41.0	33.0	41.0
24	37.21912204040116	39.0	35.0	41.0	33.0	41.0
25	37.14414763830326	39.0	35.0	41.0	33.0	41.0
26	37.03327035229295	39.0	35.0	41.0	33.0	41.0
27	36.95947826723502	39.0	35.0	41.0	33.0	41.0
28	36.94050859932647	38.0	35.0	41.0	32.0	41.0
29	36.895150562415665	38.0	35.0	41.0	32.0	41.0
30	36.81184303972358	38.0	35.0	41.0	32.0	41.0
31	36.68610374555209	38.0	35.0	41.0	32.0	41.0
32	36.52310270256637	38.0	35.0	41.0	31.0	41.0
33	36.396818693650395	38.0	35.0	41.0	31.0	41.0
34	36.27463957630974	38.0	35.0	41.0	31.0	41.0
35	36.10355516336367	38.0	35.0	41.0	30.0	41.0
36	35.963038136491186	38.0	35.0	41.0	30.0	41.0
37	35.90219617543198	38.0	35.0	41.0	30.0	41.0
38	35.84605733868554	38.0	35.0	41.0	30.0	41.0
39	35.8036692428096	38.0	35.0	41.0	30.0	41.0
40	35.658577350262654	38.0	35.0	41.0	29.0	41.0
41	35.574623920758924	38.0	35.0	40.0	29.0	41.0
42	35.56130040110813	38.0	35.0	40.0	29.0	41.0
43	35.50403061229198	38.0	35.0	40.0	29.0	41.0
44	35.390930791700285	38.0	35.0	40.0	28.0	41.0
45	35.30000464437755	37.0	34.0	40.0	28.0	41.0
46	35.25786076279134	37.0	34.0	40.0	28.0	41.0
47	35.17215815612675	37.0	34.0	40.0	27.0	41.0
48	35.10827766488079	37.0	34.0	40.0	27.0	41.0
49	35.05370561351459	37.0	34.0	40.0	27.0	41.0
50	34.913695777276565	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	10.0
11	4.0
12	8.0
13	23.0
14	16.0
15	63.0
16	150.0
17	383.0
18	980.0
19	1940.0
20	3549.0
21	5606.0
22	8473.0
23	12624.0
24	18352.0
25	27116.0
26	37891.0
27	47239.0
28	50979.0
29	54798.0
30	61904.0
31	74561.0
32	93660.0
33	125089.0
34	247861.0
35	510512.0
36	184476.0
37	238133.0
38	420853.0
39	1023988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.727869617866155	23.55883580525971	29.347391136251584	13.365903440622557
2	31.183888746550164	25.56182358562556	30.241326163562675	13.0129615042616
3	29.482078085212333	24.835424482267243	32.722746346551155	12.959751085969273
4	27.053745290647303	26.84610162943834	31.650541039227154	14.449612040687207
5	23.125217032378078	32.1055670092946	30.387731707534627	14.381484250792697
6	21.565382839732372	40.4796257923508	27.978622329982716	9.976369037934107
7	87.51938873839944	4.142692502529033	6.738222888907412	1.5996958701641188
8	88.7179457210673	3.187088753439838	6.514862161948523	1.5801033635443429
9	83.29638848895638	5.930101195142904	8.060209587533137	2.713300728367581
10	47.69828031925021	28.267281159851787	14.186574181013231	9.847864339884776
11	40.338295230470315	21.762384417282867	25.23551761587799	12.66380273636883
12	37.48351015288614	22.463162550446093	26.315073957867806	13.73825333879996
13	20.73766863934809	38.593239570219765	25.49006641459897	15.17902537583318
14	15.129106006533503	39.70589094693937	29.709806372516606	15.455196674010525
15	15.22611505814853	24.857569858666363	45.07273064486414	14.843584438320976
16	17.188810556454868	20.913232262245547	43.01281079267222	18.885146388627366
17	17.122189882454187	21.342575747183464	30.222195018951215	31.31303935141114
18	20.983789892050517	23.8296553041406	34.95709179535334	20.229463008455536
19	27.773346993749776	24.498015066852894	27.965642678815456	19.762995260581874
20	28.888735785052056	24.094200279708406	27.725365344885017	19.29169859035452
21	21.547451236342532	28.055854330174647	30.197558287707192	20.199136145775633
22	21.865606477276536	25.459985611656833	27.27147740110475	25.40293050996188
23	19.434474753194394	29.530398066216524	28.273340380894325	22.761786799694764
24	18.444391883350463	24.56208287107423	39.7443070234984	17.24921822207691
25	17.52803466243526	27.375929759787255	35.673125632258184	19.422909945519297
26	16.95954439578955	34.68928652826012	28.613887057965215	19.737282017985123
27	18.060938539506274	33.42284781543551	29.933413159213263	18.582800485844952
28	15.773259642542866	28.462468046836243	38.17167772448876	17.592594586132133
29	16.594730077081287	24.803805805964057	36.77974854540248	21.821715571552172
30	18.33526438965036	31.269240718088437	31.699383897174094	18.69611099508711
31	27.192892072355097	27.86934104894651	26.691145509578952	18.246621369119442
32	27.26354197456173	27.288609310346846	28.23209461735096	17.215754097740465
33	25.186767030332707	28.034385618054387	27.103541630078098	19.675305721534812
34	18.419847424508102	28.3805301541595	29.709806372516606	23.489816048815793
35	20.10972418856419	26.22221716432761	32.42599830280296	21.242060344305237
36	26.64430188700137	27.666187977951818	27.437906056237573	18.251604078809244
37	19.339280392145405	33.4867003173863	29.238202127617036	17.935817162851254
38	19.971930735414116	32.15588622566815	27.669540541878906	20.202642497038823
39	20.569579080985335	32.14416763065695	28.287211998610996	18.99904128974672
40	22.73047569806379	27.916369216327418	27.542604474657846	21.810550610950948
41	17.922222362339575	26.339802961513488	29.13507234002503	26.602902336121907
42	20.829356649133885	26.844871330749502	26.396058369060693	25.92971365105592
43	21.279830514052627	27.532300723138814	27.706541774945766	23.481326987862797
44	18.73397343723616	30.479450474787644	29.5415015118833	21.245074576092897
45	16.989717471133346	37.06385527012284	25.239331541813392	20.707095716930418
46	20.13995877884243	33.21591157597263	27.107478585882383	19.53665105930255
47	20.841813423358392	28.359492046580336	28.89983923071884	21.898855299342436
48	20.970687211014372	26.527577298897683	32.66618336433174	19.835552125756212
49	19.799627404042084	27.23084678690581	32.83418065029283	20.13534515875928
50	19.11638102719483	33.080609477667466	28.546466689816786	19.25654280532092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2677.0
1	5484.5
2	8292.0
3	21256.0
4	34220.0
5	24465.5
6	14711.0
7	14231.5
8	13752.0
9	13787.0
10	13822.0
11	13423.5
12	13025.0
13	12424.5
14	11824.0
15	11304.0
16	10784.0
17	10576.5
18	10369.0
19	11681.0
20	12993.0
21	12921.0
22	12849.0
23	14912.5
24	16976.0
25	22287.5
26	27599.0
27	38974.5
28	50350.0
29	59693.0
30	69036.0
31	78486.5
32	87937.0
33	93643.5
34	99350.0
35	109262.5
36	119175.0
37	128348.0
38	137521.0
39	156482.5
40	175444.0
41	220372.5
42	265301.0
43	303945.0
44	342589.0
45	340019.5
46	337450.0
47	305896.0
48	274342.0
49	264765.0
50	255188.0
51	232967.0
52	210746.0
53	188810.5
54	166875.0
55	150461.0
56	134047.0
57	118065.5
58	102084.0
59	88109.5
60	74135.0
61	65114.0
62	56093.0
63	46465.0
64	36837.0
65	28354.0
66	19871.0
67	15847.5
68	11824.0
69	10073.5
70	8323.0
71	6928.0
72	5533.0
73	4291.5
74	3050.0
75	2665.5
76	2281.0
77	1650.5
78	1020.0
79	741.0
80	462.0
81	337.0
82	212.0
83	175.0
84	138.0
85	90.0
86	42.0
87	36.5
88	31.0
89	25.5
90	20.0
91	21.0
92	22.0
93	18.0
94	14.0
95	10.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3251243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.024851212818078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67474207045696	10.377516608873588
2	7.416865383833448	1.9320713617986305
3	2.432067140092531	0.9503193791786758
4	1.1911941166729376	0.6206050454099707
5	0.7139747632541058	0.46497075305458696
6	0.5155087635805131	0.40286549667239957
7	0.3927682880503292	0.3581023959078167
8	0.31613060966189593	0.3294043323730931
9	0.2599887071845609	0.30476828052826493
>10	3.5255960584451946	11.070988155375636
>50	1.2553553300059035	11.861247005691029
>100	2.2756987155004063	57.51955032696671
>500	0.025808617081032416	2.164827879829433
>1k	0.0038234988268196174	0.897996451014478
>5k	2.3896867667622609E-4	0.166395931757076
>10k+	2.3896867667622609E-4	0.5783705955686469
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18582	0.5715352559005894	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5346	0.16442941976345662	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4137	0.12724364189326973	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3322	0.10217630610815616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02389855203071564	0.0
2	0.0	0.0	0.0	0.08467530725940817	0.0
3	0.0	0.0	0.0	0.12010790949799816	0.0
4	0.0	0.0	0.0	0.17396423460196608	0.0
5	0.0	0.0	0.0	0.3014231787657828	0.0
6	0.0	0.0	0.0	0.42534501419918475	0.0
7	0.0	0.0	0.0	0.508666992900869	0.0
8	0.0	0.0	0.0	0.7130503625844024	0.0
9	0.0	0.0	0.0	0.7912666017274008	0.0
10	0.0	0.0	0.0	0.9207247812605824	0.0
11	0.0	0.0	0.0	1.0486450874327142	0.0
12	0.0	0.0	0.0	1.1642316492492255	0.0
13	0.0	0.0	0.0	1.2067384689486451	0.0
14	0.0	0.0	0.0	1.2202717545258843	0.0
15	0.0	0.0	0.0	1.245954239655418	0.0
16	0.0	0.0	0.0	1.3044241848425355	0.0
17	0.0	0.0	0.0	1.3797799795339813	0.0
18	0.0	0.0	0.0	1.488107779086337	0.0
19	0.0	0.0	0.0	1.5385807827959952	0.0
20	0.0	0.0	0.0	1.5894843910467473	0.0
21	0.0	0.0	0.0	1.6609339874011262	0.0
22	0.0	0.0	0.0	1.7365973567647819	0.0
23	0.0	0.0	0.0	1.8251788623612568	0.0
24	0.0	0.0	0.0	1.894167861337956	0.0
25	0.0	0.0	0.0	1.9516228101067807	0.0
26	3.075746722099824E-5	0.0	0.0	2.013383804286545	0.0
27	3.075746722099824E-5	0.0	0.0	2.067732248866049	0.0
28	3.075746722099824E-5	0.0	0.0	2.1286935488980676	0.0
29	3.075746722099824E-5	0.0	0.0	2.1978363352108716	0.0
30	3.075746722099824E-5	0.0	0.0	2.2853105719873907	0.0
31	3.075746722099824E-5	0.0	0.0	2.368724823090738	0.0
32	3.075746722099824E-5	0.0	0.0	2.4446034947249404	0.0
33	3.075746722099824E-5	0.0	0.0	2.5202976215558173	0.0
34	3.075746722099824E-5	0.0	0.0	2.5996211295187717	0.0
35	3.075746722099824E-5	0.0	0.0	2.702750917110779	0.0
36	3.075746722099824E-5	0.0	0.0	2.7945927142326794	0.0
37	3.075746722099824E-5	0.0	0.0	2.8815133165992206	0.0
38	3.075746722099824E-5	0.0	0.0	2.969018310842961	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGA	20	7.8586076E-4	44.000004	20
ATACGTC	30	2.5291502E-6	44.000004	15
ACCGTAA	25	4.4446424E-5	44.0	18
CGGTCTA	385	0.0	43.42857	31
CGACGGT	385	0.0	42.285713	28
CGCATCG	95	0.0	41.68421	21
CGTTTTT	10485	0.0	40.97854	1
ACGGGAT	1335	0.0	40.37453	5
GCGATAC	60	3.6379788E-12	40.333336	9
CTCGTAG	175	0.0	40.22857	1
ACGTATA	55	7.8216544E-11	40.0	13
CAATACG	55	7.8216544E-11	40.0	1
ACGCCGA	105	0.0	39.80952	12
TAGGACG	100	0.0	39.600002	1
ACGACTT	50	1.3496901E-9	39.600002	13
TATTGCG	250	0.0	39.6	1
CCGACGG	95	0.0	39.36842	2
TACGGGT	235	0.0	39.319153	4
CGGGTAT	280	0.0	39.285713	6
GAGTACG	140	0.0	39.285713	1
>>END_MODULE