FastQCFastQC Report
Thu 26 May 2016
SRR1545908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545908_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1745443
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68720.3937109375671391No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53220.30490826684114003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.857605E-444.01
TCGCCTA207.857605E-444.015
TGTCACG254.4437922E-544.032
AACCGTA207.857605E-444.013
AGCGTAA254.4437922E-544.012
GTCGCTG302.5284753E-644.028
TCGCATA302.5284753E-644.014
GTCGCGA207.857605E-444.019
GTACCGT207.857605E-444.011
CAAACGT207.857605E-444.024
GCGACCG207.857605E-444.01
CGAGTTA207.857605E-444.013
GGTCGGC207.857605E-444.02
CGCGGTA207.857605E-444.020
CGCGGAA302.5284753E-644.033
CGTGCGT207.857605E-444.022
AACCCGC254.4437922E-544.012
ACACCGT207.857605E-444.038
CGGTTTG207.857605E-444.028
TGGCGCG254.4437922E-544.025
Produced by FastQC (version 0.11.5)