##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545908_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1745443
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793868949028987	31.0	31.0	33.0	30.0	34.0
2	32.21955285850068	33.0	31.0	34.0	31.0	34.0
3	32.28342718725275	34.0	31.0	34.0	30.0	34.0
4	35.93445503519737	37.0	35.0	37.0	35.0	37.0
5	35.911843010628246	37.0	35.0	37.0	35.0	37.0
6	35.96372668715048	37.0	35.0	37.0	35.0	37.0
7	36.20198769023108	37.0	35.0	37.0	35.0	37.0
8	36.18309449234378	37.0	36.0	37.0	35.0	37.0
9	38.01591687611684	39.0	39.0	39.0	35.0	39.0
10	37.72450604230559	39.0	38.0	39.0	35.0	39.0
11	37.34453488312136	39.0	37.0	39.0	35.0	39.0
12	36.3218420767679	37.0	35.0	39.0	33.0	39.0
13	35.99192640492986	37.0	35.0	39.0	33.0	39.0
14	36.9351310813358	38.0	35.0	41.0	33.0	41.0
15	37.198785064880376	38.0	35.0	41.0	33.0	41.0
16	37.32556204929064	38.0	35.0	41.0	33.0	41.0
17	37.29564872642647	38.0	35.0	41.0	34.0	41.0
18	37.25792821650435	38.0	35.0	41.0	33.0	41.0
19	37.1884873925989	38.0	35.0	41.0	34.0	41.0
20	37.035876278973305	38.0	35.0	41.0	34.0	41.0
21	36.8878914980323	38.0	35.0	41.0	33.0	41.0
22	36.841239158196515	38.0	35.0	41.0	33.0	41.0
23	36.85953021668425	38.0	35.0	41.0	33.0	41.0
24	36.84908816844778	38.0	35.0	41.0	33.0	41.0
25	36.754149519634844	37.0	35.0	41.0	33.0	41.0
26	36.667669468438675	37.0	35.0	41.0	33.0	41.0
27	36.58735633303408	37.0	35.0	41.0	33.0	41.0
28	36.63421091379094	37.0	35.0	41.0	33.0	41.0
29	36.66533023421561	37.0	35.0	41.0	33.0	41.0
30	36.5977216099294	37.0	35.0	41.0	32.0	41.0
31	36.394621881092654	37.0	35.0	40.0	32.0	41.0
32	36.1931778923746	37.0	35.0	40.0	31.0	41.0
33	36.04834531978415	37.0	35.0	40.0	31.0	41.0
34	35.94882731776403	37.0	35.0	40.0	31.0	41.0
35	35.75259976980056	37.0	35.0	40.0	30.0	41.0
36	35.60479889632603	37.0	35.0	40.0	30.0	41.0
37	35.516971909137105	37.0	35.0	40.0	30.0	41.0
38	35.485002947675746	36.0	35.0	40.0	30.0	41.0
39	35.48238069074728	36.0	35.0	40.0	30.0	41.0
40	35.33576003341272	36.0	35.0	40.0	29.0	41.0
41	35.289260663338766	36.0	35.0	40.0	29.0	41.0
42	35.28236270104495	36.0	35.0	40.0	29.0	41.0
43	35.232401745574045	36.0	35.0	40.0	29.0	41.0
44	35.125773800691285	36.0	34.0	40.0	29.0	41.0
45	34.99687357306999	35.0	34.0	40.0	28.0	41.0
46	34.98621381506013	35.0	34.0	40.0	28.0	41.0
47	34.952444737525084	35.0	34.0	40.0	28.0	41.0
48	34.927068944674794	35.0	34.0	40.0	28.0	41.0
49	34.92378611046021	36.0	34.0	40.0	28.0	41.0
50	34.69464428228249	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	7.0
15	20.0
16	67.0
17	151.0
18	380.0
19	813.0
20	1597.0
21	2784.0
22	4243.0
23	6444.0
24	9881.0
25	14839.0
26	21367.0
27	26092.0
28	27356.0
29	28837.0
30	33771.0
31	40700.0
32	52250.0
33	72802.0
34	155104.0
35	363994.0
36	87627.0
37	109505.0
38	193241.0
39	491564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.415463008531358	24.101789631629334	31.3599470163162	13.122800343523105
2	29.647258604262642	25.8473063858287	31.956815547686173	12.548619462222485
3	27.747626247319445	24.64085048895896	34.34910220499896	13.262421058722628
4	23.87537146730085	27.84519460102679	33.87781783764924	14.401616094023122
5	23.08892355694228	31.683704366169508	31.777949781230326	13.449422295657893
6	19.861490750485693	41.27433551253177	28.234436759034814	10.629736977947719
7	89.4027476119243	3.6940192260646723	5.852955381527784	1.0502777804832355
8	91.89260262294442	2.018284183442255	5.143393396404237	0.9457197972090752
9	88.30359971651896	4.223053975409108	6.194702433708806	1.2786438743631274
10	56.26743468563568	27.942190034277832	9.902357166633342	5.888018113453146
11	48.11867245163548	18.40180401193279	24.143269072665223	9.336254463766505
12	44.06778107334356	21.514652727130017	23.22510674940402	11.192459450122405
13	19.109933695915593	45.832433370783235	23.69822446221389	11.359408471087283
14	13.065680174030318	47.769362849431346	27.713251019941644	11.451705956596692
15	11.946021726289544	23.422993475008923	53.621630726411574	11.009354072289957
16	14.237818135567876	19.292695321474262	49.94010116629417	16.52938537666369
17	14.83806689763	17.896087125159628	30.150397348982466	37.1154486282279
18	20.47130728416797	21.59749702511053	38.04174642196852	19.889449268752976
19	28.881034786011345	23.453243675101394	28.05791996644978	19.607801572437484
20	31.611459096630483	23.428092467069963	26.731952862396536	18.228495573903015
21	21.71265403682618	29.717785112432775	29.433616566109578	19.135944284631464
22	22.10676601871273	23.713292270214495	27.501098574974947	26.67884313609783
23	16.409243956978255	32.55242365405229	27.74401684844478	23.294315540524668
24	17.267306924373926	22.84566153119867	44.64958179671293	15.237449747714477
25	13.756049323867922	25.004769562798675	42.133658904931295	19.105522208402107
26	14.70171182903137	37.441841412180175	29.36022545565796	18.496221303130493
27	15.673843259275724	36.801488218177276	31.21963879656912	16.305029725977874
28	12.640401319321226	27.913257551234842	43.54269947514757	15.90364165429636
29	12.929726149751094	24.31749418342507	41.89165730419154	20.861122362632294
30	16.62328703945073	32.907920797184445	32.97489519852553	17.493896964839298
31	29.382683937544797	26.535269269749858	26.482503295724925	17.59954349698042
32	29.8213118388856	26.736478934001283	29.109572756028122	14.332636471084992
33	28.09051914041306	28.106274452961227	27.120278347674486	16.682928058951223
34	18.3551109947446	27.50665590340103	29.863650660605934	24.27458244124844
35	18.4417938597823	25.65812805115951	34.36680544709853	21.533272641959663
36	31.681813728663727	23.29918536440319	27.96017973660555	17.058821170327533
37	18.813332775690757	33.39960113277833	30.489279798882002	17.297786292648915
38	19.305757907877826	34.14966859416205	25.954843555475605	20.589729942484517
39	18.73799373568773	33.219131189044845	29.80332213655788	18.23955293870954
40	23.946871940246687	26.387513084070918	26.473221984332916	23.192392991349475
41	15.879693579223153	24.483755699842387	29.830707734368865	29.80584298656559
42	21.43811055416877	24.97268601724605	26.769536444329606	26.819666984255576
43	21.904410513548708	26.29882499743618	27.175278711478978	24.621485777536133
44	18.627706547850604	30.933808780922668	30.40821155431601	20.03027311691072
45	15.398268519796979	40.667555457267866	24.11296158052712	19.82121444240803
46	20.588068473161254	34.58589022958641	27.10400740671566	17.72203389053667
47	20.526422232063723	27.937778546764346	27.47990051809197	24.055898703079965
48	22.257730558946925	24.373525804050892	33.288339980165496	20.080403656836687
49	21.371479905101456	23.203106603882222	35.33057223868095	20.094841252335367
50	19.21643960874116	35.47638049480848	26.35989831807742	18.94728157837294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1931.0
1	2434.5
2	2938.0
3	7915.5
4	12893.0
5	9884.0
6	6875.0
7	7224.5
8	7574.0
9	8048.5
10	8523.0
11	8373.0
12	8223.0
13	7661.0
14	7099.0
15	6736.0
16	6373.0
17	5699.5
18	5026.0
19	5093.5
20	5161.0
21	5809.0
22	6457.0
23	9032.5
24	11608.0
25	12317.5
26	13027.0
27	16947.5
28	20868.0
29	24543.0
30	28218.0
31	31620.5
32	35023.0
33	42148.0
34	49273.0
35	52856.0
36	56439.0
37	63370.5
38	70302.0
39	89235.0
40	108168.0
41	142128.0
42	176088.0
43	199222.5
44	222357.0
45	224809.0
46	227261.0
47	199016.0
48	170771.0
49	153315.0
50	135859.0
51	126062.5
52	116266.0
53	100870.5
54	85475.0
55	71054.0
56	56633.0
57	45690.0
58	34747.0
59	28761.5
60	22776.0
61	17205.0
62	11634.0
63	9321.0
64	7008.0
65	5349.0
66	3690.0
67	2534.0
68	1378.0
69	1017.5
70	657.0
71	544.0
72	431.0
73	318.0
74	205.0
75	149.0
76	93.0
77	70.0
78	47.0
79	33.5
80	20.0
81	18.5
82	17.0
83	11.5
84	6.0
85	7.5
86	9.0
87	7.0
88	5.0
89	4.0
90	3.0
91	4.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1745443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.637599246405596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96574457525536	10.105750333825094
2	7.329853182779909	1.8526349411792604
3	2.2471313262888475	0.8519503546704703
4	1.0919820693740179	0.5520012710803804
5	0.6802443947347179	0.42983280251355505
6	0.4579327205544408	0.3472302122509959
7	0.360019204789437	0.3184844901796971
8	0.2883278033127885	0.2915016983890782
9	0.22553278633287496	0.25651736735400854
>10	3.2284909729512155	10.087700733232072
>50	1.4894388243917718	13.702421287715216
>100	2.6155775105162213	58.97032389782319
>500	0.01788977953527405	1.3729667291841594
>1k	9.174245915525153E-4	0.15379499799550647
>5k	9.174245915525153E-4	0.7068888826073146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6872	0.3937109375671391	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5322	0.30490826684114003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04084922853395957	0.0
2	0.0	0.0	0.0	0.1300529435793664	0.0
3	0.0	0.0	0.0	0.17806367781703555	0.0
4	0.0	0.0	0.0	0.24985061099102063	0.0
5	0.0	0.0	0.0	0.39886722167381006	0.0
6	0.0	0.0	0.0	0.5303524663939183	0.0
7	0.0	0.0	0.0	0.6215614030363639	0.0
8	0.0	0.0	0.0	0.8706672174341986	0.0
9	0.0	0.0	0.0	0.9658865972707216	0.0
10	0.0	0.0	0.0	1.1428044341751635	0.0
11	0.0	0.0	0.0	1.2883835221201723	0.0
12	0.0	0.0	0.0	1.4266292282245825	0.0
13	0.0	0.0	0.0	1.4775618567893651	0.0
14	0.0	0.0	0.0	1.4925150806987109	0.0
15	0.0	0.0	0.0	1.5182392091864358	0.0
16	0.0	0.0	0.0	1.5854427787100467	0.0
17	0.0	0.0	0.0	1.672068351702118	0.0
18	0.0	0.0	0.0	1.8236058123926133	0.0
19	0.0	0.0	0.0	1.8853093455357752	0.0
20	0.0	0.0	0.0	1.9505076934623473	0.0
21	0.0	0.0	0.0	2.0276800789255223	0.0
22	0.0	0.0	0.0	2.109722288267219	0.0
23	0.0	0.0	0.0	2.216170909047159	0.0
24	0.0	0.0	0.0	2.290593276320109	0.0
25	0.0	0.0	0.0	2.349775959455565	0.0
26	0.0	0.0	0.0	2.415088891473397	0.0
27	0.0	0.0	0.0	2.471922600738036	0.0
28	0.0	0.0	0.0	2.53500114297631	0.0
29	0.0	0.0	0.0	2.603465137503774	0.0
30	0.0	0.0	0.0	2.7096272980555653	0.0
31	0.0	0.0	0.0	2.806049810850311	0.0
32	0.0	0.0	0.0	2.883394072450375	0.0
33	0.0	0.0	0.0	2.964748777244516	0.0
34	0.0	0.0	0.0	3.0465618184036947	0.0
35	0.0	0.0	0.0	3.1758126733442458	0.0
36	0.0	0.0	0.0	3.2681674508992846	0.0
37	0.0	0.0	0.0	3.3634441227814373	0.0
38	0.0	0.0	0.0	3.4580332901160338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.857605E-4	44.0	1
TCGCCTA	20	7.857605E-4	44.0	15
TGTCACG	25	4.4437922E-5	44.0	32
AACCGTA	20	7.857605E-4	44.0	13
AGCGTAA	25	4.4437922E-5	44.0	12
GTCGCTG	30	2.5284753E-6	44.0	28
TCGCATA	30	2.5284753E-6	44.0	14
GTCGCGA	20	7.857605E-4	44.0	19
GTACCGT	20	7.857605E-4	44.0	11
CAAACGT	20	7.857605E-4	44.0	24
GCGACCG	20	7.857605E-4	44.0	1
CGAGTTA	20	7.857605E-4	44.0	13
GGTCGGC	20	7.857605E-4	44.0	2
CGCGGTA	20	7.857605E-4	44.0	20
CGCGGAA	30	2.5284753E-6	44.0	33
CGTGCGT	20	7.857605E-4	44.0	22
AACCCGC	25	4.4437922E-5	44.0	12
ACACCGT	20	7.857605E-4	44.0	38
CGGTTTG	20	7.857605E-4	44.0	28
TGGCGCG	25	4.4437922E-5	44.0	25
>>END_MODULE