FastQCFastQC Report
Thu 26 May 2016
SRR1545907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545907_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1656604
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89520.5403826140707133No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA62470.37709675939452036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG302.5283953E-644.0000041
CGAACAG302.5283953E-644.00000416
CGTGACA408.3164196E-944.00000413
CGCATCG207.8574877E-444.00000421
CACGCTT302.5283953E-644.00000413
ACCGACG207.8574877E-444.00000434
GCCGATT600.044.0000049
CCCGTCG207.8574877E-444.00000425
GCCCTCG207.8574877E-444.0000041
ACGCTTA302.5283953E-644.00000414
ACTCGTC207.8574877E-444.00000441
CGTACCG207.8574877E-444.00000419
CGTCGAC302.5283953E-644.00000411
ACGTATC207.8574877E-444.0000049
CCGAATT207.8574877E-444.00000420
CGCAATA207.8574877E-444.00000419
CCTATCA302.5283953E-644.00000410
CGCAAAC207.8574877E-444.00000423
TATCGTC207.8574877E-444.00000438
GCGTATC207.8574877E-444.00000423
Produced by FastQC (version 0.11.5)