##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545907_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1656604
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75774657069523	31.0	31.0	33.0	30.0	34.0
2	32.17495973690755	33.0	31.0	34.0	30.0	34.0
3	32.2442533037467	34.0	31.0	34.0	30.0	34.0
4	35.91131435152879	37.0	35.0	37.0	35.0	37.0
5	35.88868733867599	37.0	35.0	37.0	35.0	37.0
6	35.936078266139646	37.0	35.0	37.0	35.0	37.0
7	36.13429823904808	37.0	35.0	37.0	35.0	37.0
8	36.091456377021906	37.0	35.0	37.0	35.0	37.0
9	37.91116223309856	39.0	39.0	39.0	35.0	39.0
10	37.65656547974048	39.0	37.0	39.0	35.0	39.0
11	37.3442560805117	39.0	37.0	39.0	35.0	39.0
12	36.13972500368223	37.0	35.0	39.0	33.0	39.0
13	35.78105811648408	37.0	35.0	39.0	32.0	39.0
14	36.662674966377	38.0	35.0	41.0	33.0	41.0
15	36.95461256884566	38.0	35.0	41.0	33.0	41.0
16	37.091917561469124	38.0	35.0	41.0	33.0	41.0
17	37.04546831952597	38.0	35.0	41.0	33.0	41.0
18	37.01807070368054	38.0	35.0	41.0	33.0	41.0
19	36.94361356123733	37.0	35.0	41.0	33.0	41.0
20	36.74016240453361	36.0	35.0	41.0	33.0	41.0
21	36.585204430268185	36.0	35.0	41.0	33.0	41.0
22	36.568700787876885	36.0	35.0	41.0	33.0	41.0
23	36.57913659510662	36.0	35.0	41.0	33.0	41.0
24	36.5338880022021	36.0	35.0	41.0	33.0	41.0
25	36.42449674152664	36.0	35.0	40.0	33.0	41.0
26	36.30213497009545	35.0	35.0	40.0	32.0	41.0
27	36.211896747804545	36.0	35.0	40.0	32.0	41.0
28	36.2514608198459	36.0	35.0	40.0	32.0	41.0
29	36.256159589135365	36.0	35.0	40.0	32.0	41.0
30	36.1893620925701	36.0	35.0	40.0	32.0	41.0
31	35.962051280813036	36.0	35.0	40.0	31.0	41.0
32	35.74599481831506	35.0	35.0	40.0	31.0	41.0
33	35.58937199234096	35.0	35.0	40.0	30.0	41.0
34	35.45498743212017	35.0	35.0	40.0	30.0	41.0
35	35.265056102725815	35.0	35.0	40.0	30.0	41.0
36	35.09963334629157	35.0	35.0	40.0	29.0	41.0
37	35.0215615801966	35.0	34.0	40.0	29.0	41.0
38	34.979316119000075	35.0	34.0	40.0	29.0	41.0
39	34.96297183877378	35.0	34.0	40.0	29.0	41.0
40	34.77924174998974	35.0	34.0	40.0	27.0	41.0
41	34.74116747273337	35.0	34.0	40.0	27.0	41.0
42	34.71704764687276	35.0	34.0	40.0	27.0	41.0
43	34.64436823767177	35.0	34.0	40.0	27.0	41.0
44	34.51199985029615	35.0	34.0	40.0	27.0	41.0
45	34.395579752312564	35.0	34.0	40.0	27.0	41.0
46	34.40651899910902	35.0	34.0	40.0	27.0	41.0
47	34.35152999751298	35.0	34.0	40.0	27.0	41.0
48	34.311702132796974	35.0	34.0	40.0	26.0	41.0
49	34.29003189657878	35.0	34.0	39.0	26.0	41.0
50	34.043712317488065	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	13.0
14	23.0
15	46.0
16	115.0
17	233.0
18	533.0
19	1087.0
20	2027.0
21	3355.0
22	5118.0
23	7331.0
24	10719.0
25	15535.0
26	21961.0
27	27547.0
28	29640.0
29	31095.0
30	35295.0
31	42201.0
32	53405.0
33	73122.0
34	158107.0
35	380584.0
36	85405.0
37	104994.0
38	178565.0
39	388540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.87182694234711	22.66045476166905	30.79740239671038	13.670315899273453
2	31.00572013589246	25.10159338019225	31.27126337978177	12.621423104133516
3	29.01689238949079	24.377400996254988	33.3476799524811	13.25802666177312
4	25.860253868758015	26.535490678520635	32.42778600075818	15.176469451963174
5	22.784564084114248	32.10882021291751	31.070189375372752	14.03642632759549
6	20.129312738590514	40.26321317587064	28.92779445178208	10.67967963375677
7	87.11285255860786	4.369964095221309	6.926881741200673	1.590301604970168
8	89.07898326938725	2.80145405902678	6.526303208250131	1.5932594633358363
9	85.303367612296	3.9270097138483306	8.138637839821708	2.6309848340339634
10	59.87200320655993	17.47605342012937	12.833302346245693	9.818641027065008
11	53.70957694174348	17.304678728289925	17.849105760942265	11.136638569024342
12	45.591221559286346	20.443147547633593	22.277985565651175	11.687645327428884
13	18.8848391045778	46.5856656147154	22.84076339306195	11.688731887644845
14	12.823342210932728	46.209595051080406	29.437391193067263	11.529671544919607
15	11.538303662190843	19.807751279122833	56.302411439305956	12.35153361938037
16	13.14683533300656	14.615683651614992	53.126818479250325	19.110662536128128
17	14.522299837498883	14.66850255100193	29.74675903233362	41.06243857916557
18	21.36479206859334	19.931679508198698	38.165669043416536	20.537859379791428
19	31.34557202566214	21.63516446899802	26.06724358989837	20.95201991544147
20	32.84061851836649	20.87300284195861	25.80230399057349	20.484074649101412
21	21.196194141750233	28.41964645745151	29.44191852730043	20.94224087349783
22	22.46964271485521	23.1439740577712	25.392248237961518	28.994134989412075
23	17.44490536060519	30.00753348416399	25.832546583251037	26.715014571979783
24	18.680505419520898	21.130879799879754	43.39154076653202	16.797074014067334
25	15.40742386231109	22.854526489130773	41.31965152806585	20.418398120492284
26	14.759592515773232	35.71782997022825	29.233419694749017	20.2891578192495
27	15.94201148856335	35.76346549929856	30.43612112490372	17.858401887234365
28	13.169472004172391	27.479349319451117	42.92691554529628	16.42426313108021
29	13.690477627725153	23.35349908608213	41.24540324664193	21.710620039550793
30	17.950940598960283	30.92145135469913	32.83397842815784	18.29362961818274
31	32.68741352791615	25.11306262691627	24.6060615572581	17.59346228790948
32	33.32619020598767	24.508029679995943	26.968122737841995	15.197657376174389
33	30.134962851713503	26.14626066338123	24.43263447389962	19.286142011005648
34	18.770267366250472	27.294392624912167	28.508321843965124	25.427018164872234
35	20.2825780934973	25.03706377625552	33.21928475362851	21.46107337661867
36	33.04706495939887	23.43118814152326	26.097546547032362	17.42420035204551
37	19.62593353631888	34.01712177442527	29.344611023515576	17.01233366574027
38	20.180501797653513	34.75682782366818	23.07201962569208	21.99065075298623
39	20.705672568700788	32.4027951157911	27.0929564337645	19.798575881743616
40	24.65857863436283	24.286311031483688	25.408788099026687	25.6463222351268
41	16.401445366545055	23.159306629707523	27.927676137447456	32.511571866299974
42	22.831406902313407	23.425634611530576	24.622118502671732	29.120839983484288
43	22.63938756637072	24.716588877003797	26.787572648623325	25.856450908002156
44	18.883873273274723	30.779111966408383	29.715007328245015	20.622007432071875
45	16.161074100992153	40.52760949508754	22.99300255220922	20.31831385171109
46	22.6100504405398	32.8106173835147	26.262824428771147	18.31650774717434
47	21.494636014400545	26.944399506460204	27.08661816583806	24.474346313301186
48	23.100451284676364	23.425815704899904	33.18185879063434	20.2918742197894
49	21.33491166265444	23.557229126574608	34.239383703045505	20.868475507725442
50	19.94206219470676	34.869045348194255	26.152357473481896	19.036534983617088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1687.0
1	2667.0
2	3647.0
3	10153.0
4	16659.0
5	12093.5
6	7528.0
7	8045.5
8	8563.0
9	8574.0
10	8585.0
11	8253.5
12	7922.0
13	7257.0
14	6592.0
15	6072.0
16	5552.0
17	5569.5
18	5587.0
19	5090.5
20	4594.0
21	4356.0
22	4118.0
23	4466.0
24	4814.0
25	5889.5
26	6965.0
27	8810.5
28	10656.0
29	13301.0
30	15946.0
31	19658.0
32	23370.0
33	27019.0
34	30668.0
35	34130.5
36	37593.0
37	40660.0
38	43727.0
39	57601.5
40	71476.0
41	110283.5
42	149091.0
43	176542.0
44	203993.0
45	205885.5
46	207778.0
47	184263.0
48	160748.0
49	148979.5
50	137211.0
51	129910.0
52	122609.0
53	108903.0
54	95197.0
55	82956.5
56	70716.0
57	65407.0
58	60098.0
59	51111.5
60	42125.0
61	36401.5
62	30678.0
63	25041.0
64	19404.0
65	15885.0
66	12366.0
67	9491.5
68	6617.0
69	5588.0
70	4559.0
71	3694.0
72	2829.0
73	2449.5
74	2070.0
75	1573.5
76	1077.0
77	804.5
78	532.0
79	444.0
80	356.0
81	242.0
82	128.0
83	113.0
84	98.0
85	70.5
86	43.0
87	29.0
88	15.0
89	10.5
90	6.0
91	5.5
92	5.0
93	4.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1656604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.756845584923532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05437107366977	11.012956212586442
2	7.171069804979224	1.9730259997161381
3	2.3145340051538543	0.9552206072986853
4	1.1566094645863731	0.6364519122550326
5	0.7602689992718555	0.5229451612993629
6	0.5029989057198674	0.41518069652242356
7	0.40014091355837134	0.3853273732022925
8	0.34392232829048025	0.3785029090799612
9	0.28679828283009823	0.35508957218233983
>10	3.568065351219335	11.6869049378655
>50	1.161711454048493	11.61217513439874
>100	2.2573308856675247	57.262106523613944
>500	0.019960677904310987	1.6732047388120082
>1k	0.001330711860287399	0.20344509843906763
>5k	8.871412401915994E-4	0.9274631227280712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8952	0.5403826140707133	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6247	0.37709675939452036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024689062684866148	0.0
2	0.0	0.0	0.0	0.08897720879582567	0.0
3	0.0	0.0	0.0	0.12628244287711488	0.0
4	0.0	0.0	0.0	0.18960475768499896	0.0
5	0.0	0.0	0.0	0.3251229623977728	0.0
6	0.0	0.0	0.0	0.46305574536823524	0.0
7	0.0	0.0	0.0	0.5564395594843426	0.0
8	0.0	0.0	0.0	0.8132903216459697	0.0
9	0.0	0.0	0.0	0.9132538615142786	0.0
10	0.0	0.0	0.0	1.0792561167303714	0.0
11	0.0	0.0	0.0	1.213023752206321	0.0
12	0.0	0.0	0.0	1.339366559539878	0.0
13	0.0	0.0	0.0	1.3922458233832589	0.0
14	0.0	0.0	0.0	1.4084838621662148	0.0
15	0.0	0.0	0.0	1.438786819300207	0.0
16	0.0	0.0	0.0	1.511103438117981	0.0
17	0.0	0.0	0.0	1.5930783699665099	0.0
18	0.0	0.0	0.0	1.727328921093997	0.0
19	0.0	0.0	0.0	1.7789405313520914	0.0
20	0.0	0.0	0.0	1.8386409787734426	0.0
21	0.0	0.0	0.0	1.913009989110252	0.0
22	0.0	0.0	0.0	1.9865338970568707	0.0
23	6.036445644221552E-5	0.0	0.0	2.087463268228255	0.0
24	6.036445644221552E-5	0.0	0.0	2.1520532366214256	0.0
25	6.036445644221552E-5	0.0	0.0	2.2078299943740327	0.0
26	6.036445644221552E-5	0.0	0.0	2.2649347701683684	0.0
27	6.036445644221552E-5	0.0	0.0	2.32083225683386	0.0
28	6.036445644221552E-5	0.0	0.0	2.3847582162061665	0.0
29	6.036445644221552E-5	0.0	0.0	2.448865268947799	0.0
30	6.036445644221552E-5	0.0	0.0	2.5376010199178562	0.0
31	6.036445644221552E-5	0.0	0.0	2.6234392769786865	0.0
32	6.036445644221552E-5	0.0	0.0	2.7054745732836576	0.0
33	6.036445644221552E-5	0.0	0.0	2.7771271830805673	0.0
34	6.036445644221552E-5	0.0	0.0	2.8620599732947642	0.0
35	6.036445644221552E-5	0.0	0.0	2.977114627273627	0.0
36	6.036445644221552E-5	0.0	0.0	3.0580633633626384	0.0
37	6.036445644221552E-5	0.0	0.0	3.1429961535768354	0.0
38	6.036445644221552E-5	0.0	0.0	3.221530311408158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	30	2.5283953E-6	44.000004	1
CGAACAG	30	2.5283953E-6	44.000004	16
CGTGACA	40	8.3164196E-9	44.000004	13
CGCATCG	20	7.8574877E-4	44.000004	21
CACGCTT	30	2.5283953E-6	44.000004	13
ACCGACG	20	7.8574877E-4	44.000004	34
GCCGATT	60	0.0	44.000004	9
CCCGTCG	20	7.8574877E-4	44.000004	25
GCCCTCG	20	7.8574877E-4	44.000004	1
ACGCTTA	30	2.5283953E-6	44.000004	14
ACTCGTC	20	7.8574877E-4	44.000004	41
CGTACCG	20	7.8574877E-4	44.000004	19
CGTCGAC	30	2.5283953E-6	44.000004	11
ACGTATC	20	7.8574877E-4	44.000004	9
CCGAATT	20	7.8574877E-4	44.000004	20
CGCAATA	20	7.8574877E-4	44.000004	19
CCTATCA	30	2.5283953E-6	44.000004	10
CGCAAAC	20	7.8574877E-4	44.000004	23
TATCGTC	20	7.8574877E-4	44.000004	38
GCGTATC	20	7.8574877E-4	44.000004	23
>>END_MODULE