FastQCFastQC Report
Thu 26 May 2016
SRR1545905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545905_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences810739
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90201.1125652028581332No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11500.14184589615153584No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8590.10595271721232111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTC207.855108E-444.012
GTTGACG351.4453872E-744.01
ATTCGCG302.526791E-644.01
CGCGATT207.855108E-444.023
TAACGAG254.4416753E-544.01
ATCGTTA254.4416753E-544.012
ACTACGG254.4416753E-544.02
GCGATAA302.526791E-644.09
TCGTACA207.855108E-444.034
TTACGAG351.4453872E-744.01
CGTTTTT46350.042.5760541
CGTTGAT1500.041.06666625
CGACCCG1250.040.4832
ACAACGG603.6379788E-1240.3333322
AATACGG501.3460522E-939.6000022
AGGGCGA5050.039.207926
ACCCGCT1300.038.9230834
ACGGGTA800.038.55
TCGACTG404.1225394E-738.51
TCGAAGG1600.038.52
Produced by FastQC (version 0.11.5)