FastQCFastQC Report
Thu 26 May 2016
SRR1545904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545904_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1399956
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71200.5085874127472577No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44030.314509884596373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCA302.5281133E-644.00000412
CACTCGA302.5281133E-644.00000433
CGCTATT302.5281133E-644.00000442
ATATACG302.5281133E-644.0000041
AACGCCA302.5281133E-644.00000417
AGATTCG302.5281133E-644.0000041
ACTCGAA302.5281133E-644.00000434
TCGTAAT600.044.00000424
CGTAATA600.044.00000425
AATACGC302.5281133E-644.00000411
TACCGCG302.5281133E-644.00000412
AACGTAG408.314601E-944.01
AGCCGTC207.8570715E-444.033
ACTATCG502.7284841E-1144.01
ATTAGCG502.7284841E-1144.01
CGAGTTA254.443339E-544.026
TTTACGT207.8570715E-444.030
CAACGAA254.443339E-544.041
CGACGAA408.314601E-944.043
CTATCGC207.8570715E-444.044
Produced by FastQC (version 0.11.5)