##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545904_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1399956
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75674164045156	31.0	31.0	33.0	30.0	34.0
2	32.183945066844956	33.0	31.0	34.0	30.0	34.0
3	32.25107431947861	34.0	31.0	34.0	30.0	34.0
4	35.91920531788142	37.0	35.0	37.0	35.0	37.0
5	35.90360197034764	37.0	35.0	37.0	35.0	37.0
6	35.94647974650632	37.0	35.0	37.0	35.0	37.0
7	36.1244389109372	37.0	35.0	37.0	35.0	37.0
8	36.08844992271186	37.0	35.0	37.0	35.0	37.0
9	37.942924634774236	39.0	39.0	39.0	35.0	39.0
10	37.6879173345448	39.0	38.0	39.0	35.0	39.0
11	37.36442788201915	39.0	37.0	39.0	35.0	39.0
12	36.21474389195089	37.0	35.0	39.0	33.0	39.0
13	35.863705716465375	37.0	35.0	39.0	33.0	39.0
14	36.751698624813926	38.0	35.0	41.0	33.0	41.0
15	37.01280754538	38.0	35.0	41.0	33.0	41.0
16	37.15564703462109	38.0	35.0	41.0	33.0	41.0
17	37.10721908402836	38.0	35.0	41.0	33.0	41.0
18	37.09553443108212	38.0	35.0	41.0	33.0	41.0
19	36.9873253159385	37.0	35.0	41.0	33.0	41.0
20	36.80282808888279	37.0	35.0	41.0	33.0	41.0
21	36.65258550983031	36.0	35.0	41.0	33.0	41.0
22	36.62437533751061	37.0	35.0	41.0	33.0	41.0
23	36.659205003585825	36.0	35.0	41.0	33.0	41.0
24	36.60407898533954	36.0	35.0	41.0	33.0	41.0
25	36.53034952527079	36.0	35.0	41.0	33.0	41.0
26	36.41744169102458	36.0	35.0	40.0	33.0	41.0
27	36.336772012834686	36.0	35.0	40.0	32.0	41.0
28	36.395371711682365	36.0	35.0	40.0	32.0	41.0
29	36.395193134641374	36.0	35.0	40.0	32.0	41.0
30	36.32291300583733	36.0	35.0	40.0	32.0	41.0
31	36.10338396349599	36.0	35.0	40.0	32.0	41.0
32	35.89175802668084	36.0	35.0	40.0	31.0	41.0
33	35.72416847386632	36.0	35.0	40.0	31.0	41.0
34	35.593686515861926	36.0	35.0	40.0	30.0	41.0
35	35.40982645168848	36.0	35.0	40.0	30.0	41.0
36	35.2255170876799	36.0	35.0	40.0	29.0	41.0
37	35.1581171122521	35.0	34.0	40.0	29.0	41.0
38	35.1480139375809	35.0	34.0	40.0	29.0	41.0
39	35.16333513338991	36.0	35.0	40.0	29.0	41.0
40	34.98438879507642	36.0	34.0	40.0	28.0	41.0
41	34.93472009120287	35.0	34.0	40.0	29.0	41.0
42	34.910529330921825	35.0	34.0	40.0	28.0	41.0
43	34.8507588809934	35.0	34.0	40.0	28.0	41.0
44	34.725332796173596	35.0	34.0	40.0	27.0	41.0
45	34.62612753543683	35.0	34.0	40.0	27.0	41.0
46	34.61118992311187	35.0	34.0	40.0	27.0	41.0
47	34.557652526222256	35.0	34.0	40.0	27.0	41.0
48	34.5519416324513	35.0	34.0	40.0	27.0	41.0
49	34.52154353422536	36.0	34.0	40.0	27.0	41.0
50	34.29627645440285	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	5.0
14	9.0
15	17.0
16	69.0
17	157.0
18	461.0
19	966.0
20	1629.0
21	2598.0
22	3958.0
23	5862.0
24	8747.0
25	13112.0
26	18393.0
27	22725.0
28	24183.0
29	25050.0
30	28606.0
31	34095.0
32	43274.0
33	59281.0
34	130725.0
35	319060.0
36	69908.0
37	84542.0
38	147823.0
39	354694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.525879384780666	23.314232733028753	30.659535013957584	13.500352868233001
2	31.18762303958124	24.173331161836515	31.15855069730763	13.480495101274611
3	28.61268497009906	24.273120012343245	33.65012900405441	13.464066013503281
4	25.0858598413093	27.12135238536068	33.24311621222381	14.549671561106207
5	22.815288480495102	31.852858232687314	31.726211395215277	13.605641891602307
6	21.185880127661154	39.81646566034932	29.09698590527131	9.900668306718211
7	87.283957495807	4.60793053495967	6.6754240847569495	1.4326878844763693
8	89.5816725668521	2.6521547820074343	6.337699184831523	1.4284734663089411
9	86.1734225932815	3.9471240524702202	7.72459991599736	2.1548534382509166
10	58.9888539354094	19.759906739926112	13.024623631028403	8.226615693636086
11	52.75537231170122	17.222684141501592	19.15360197034764	10.868341576449545
12	46.39381523419308	20.93415793067782	21.781684567229256	10.890342267899848
13	20.063416278797334	48.03186671581107	20.139418667443834	11.765298337947765
14	12.70004200132004	47.64485455257165	26.719768335576262	12.935335110532046
15	11.590435699407696	21.433745060559044	54.577429576358114	12.398389663675143
16	12.405889899396838	15.348268088425638	52.03427822017264	20.211563792004892
17	13.119340893570941	16.0872198840535	29.860867055821753	40.932572166553804
18	20.068987882476303	20.870584504084412	38.21255810896914	20.84786950447014
19	30.46731468703302	22.272842860775626	26.44218818305718	20.817654269134174
20	33.088539925540516	20.634219932626454	26.670052487363886	19.60718765446914
21	20.338924937640897	29.026555120303783	29.048984396652465	21.585535545402855
22	21.577392432333586	24.176831271839973	24.71484818094283	29.530928114883608
23	17.696056161765085	30.565746352028206	25.462157382089153	26.27604010411756
24	16.738954652860517	21.543105640462986	46.184451511333215	15.533488195343281
25	15.013114697890506	24.371123092440047	40.82885462114524	19.786907588524212
26	14.680175662663682	36.860158462123096	28.098740246122023	20.360925629091202
27	14.783250330724679	38.33699059113286	29.413995868441578	17.465763209700878
28	12.196383314904184	28.54996871330242	43.191857458377264	16.061790513416135
29	12.192883204900726	22.39041798456523	42.41483303760975	23.001865772924294
30	15.735423113297847	32.545737151739054	33.90156547777216	17.81727425719094
31	31.082691170293923	27.03370677364146	24.685347253770836	17.198254802293786
32	32.18551154464855	25.445442571052233	26.522690713136697	15.846355171162521
33	29.67186111563506	26.99320550074431	24.54348565240622	18.791447731214408
34	18.101640337267742	26.750555017443407	28.558540411270066	26.589264234018785
35	18.653514824751635	24.723848463808864	33.70320210063745	22.919434610802053
36	32.352945378283316	24.361622793859237	26.44740263265417	16.838029195203276
37	18.764518313432706	34.52265642634483	30.285594690118835	16.42723057010363
38	18.42807916820243	34.13150127575438	25.64123443879665	21.79918511724654
39	18.19978627899734	34.44136815728494	28.340319267176966	19.01852629654075
40	24.300120860941345	25.607233370191633	26.08446265454057	24.00818311432645
41	15.99671703967839	23.718388292203468	28.32639025797954	31.958504410138605
42	20.751009317435688	24.51641337299172	24.573486595292994	30.15909071427959
43	21.082876890416554	25.339153516253365	26.65776638694359	26.920203206386482
44	17.760272465706066	30.670463928866337	30.016943389649388	21.55232021577821
45	15.064259162430819	42.27732871604536	21.78361319927198	20.87479892225184
46	20.835511973233444	34.97967078965339	25.514301878059026	18.67051535905414
47	19.795836440573847	28.2733171613965	26.734125929672075	25.196720468357576
48	21.281025975102075	22.97150767595553	33.70998802819517	22.037478320747223
49	19.64054584572658	24.85806696781899	35.01788627642583	20.483500910028603
50	18.098925966244654	36.271282811745515	27.210855198306234	18.4189360237036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1369.0
1	2107.0
2	2845.0
3	7868.0
4	12891.0
5	9608.5
6	6326.0
7	6628.5
8	6931.0
9	7062.0
10	7193.0
11	7031.0
12	6869.0
13	6391.0
14	5913.0
15	5291.5
16	4670.0
17	4363.0
18	4056.0
19	4050.0
20	4044.0
21	4629.0
22	5214.0
23	4769.0
24	4324.0
25	5013.5
26	5703.0
27	9250.0
28	12797.0
29	15882.0
30	18967.0
31	21070.5
32	23174.0
33	26028.0
34	28882.0
35	34684.0
36	40486.0
37	42741.0
38	44996.0
39	57651.0
40	70306.0
41	100742.5
42	131179.0
43	155346.5
44	179514.0
45	177197.0
46	174880.0
47	152876.0
48	130872.0
49	122981.0
50	115090.0
51	107187.5
52	99285.0
53	86025.0
54	72765.0
55	64524.0
56	56283.0
57	47331.5
58	38380.0
59	34638.0
60	30896.0
61	25635.0
62	20374.0
63	16541.0
64	12708.0
65	10362.5
66	8017.0
67	6325.0
68	4633.0
69	3734.0
70	2835.0
71	2379.0
72	1923.0
73	1493.0
74	1063.0
75	837.5
76	612.0
77	447.5
78	283.0
79	223.5
80	164.0
81	143.5
82	123.0
83	89.0
84	55.0
85	49.0
86	43.0
87	29.0
88	15.0
89	10.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1399956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.617401008419858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10455772074054	10.90815885085439
2	6.934563398754051	1.888614612382897
3	2.1658007681234093	0.8847773269164064
4	1.0669733100410232	0.5811761371243882
5	0.6630866810694009	0.45147586197321177
6	0.4862500748457915	0.39728773557295877
7	0.38833819449088275	0.37017098448876656
8	0.34384162172900634	0.3745783397175432
9	0.27750039242107966	0.3400950711232545
>10	3.744390775294281	12.244306719340974
>50	1.417300435850825	14.207692369565454
>100	2.3989161453480943	55.85415167127148
>500	0.007420421130108997	0.6658265663649477
>1k	5.300300807220712E-4	0.3177923438162455
>5k	5.300300807220712E-4	0.5138954094870924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7120	0.5085874127472577	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4403	0.314509884596373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04364422881862001	0.0
2	0.0	0.0	0.0	0.13036123992468335	0.0
3	0.0	0.0	0.0	0.17986279568786448	0.0
4	0.0	0.0	0.0	0.23922180411384358	0.0
5	0.0	0.0	0.0	0.3646543177071279	0.0
6	0.0	0.0	0.0	0.4900868313004123	0.0
7	0.0	0.0	0.0	0.5708036538291203	0.0
8	0.0	0.0	0.0	0.7418090282837461	0.0
9	0.0	0.0	0.0	0.8165970930514959	0.0
10	0.0	0.0	0.0	0.9281720282637455	0.0
11	0.0	0.0	0.0	1.0607476234967386	0.0
12	0.0	0.0	0.0	1.179679932797888	0.0
13	0.0	0.0	0.0	1.2279671646823185	0.0
14	0.0	0.0	0.0	1.2467534693947524	0.0
15	0.0	0.0	0.0	1.276040104117558	0.0
16	0.0	0.0	0.0	1.3486852443933952	0.0
17	0.0	0.0	0.0	1.432402161210781	0.0
18	0.0	0.0	0.0	1.569906482775173	0.0
19	0.0	0.0	0.0	1.6249796422173268	0.0
20	0.0	0.0	0.0	1.6831243267645555	0.0
21	0.0	0.0	0.0	1.754340850712451	0.0
22	0.0	0.0	0.0	1.833414764464026	0.0
23	0.0	0.0	0.0	1.9347750929314922	0.0
24	0.0	0.0	0.0	2.0053487395318137	0.0
25	0.0	0.0	0.0	2.057278943052496	0.0
26	0.0	0.0	0.0	2.111923517596267	0.0
27	0.0	0.0	0.0	2.1664966613236416	0.0
28	0.0	0.0	0.0	2.223069867910134	0.0
29	0.0	0.0	0.0	2.2800002285786123	0.0
30	0.0	0.0	0.0	2.366217223969896	0.0
31	0.0	0.0	0.0	2.4429339207803675	0.0
32	0.0	0.0	0.0	2.5082216869673046	0.0
33	0.0	0.0	0.0	2.569366465803211	0.0
34	0.0	0.0	0.0	2.6445116846529464	0.0
35	0.0	0.0	0.0	2.766086934160788	0.0
36	0.0	0.0	0.0	2.8463751717911134	0.0
37	0.0	0.0	0.0	2.9223775604376137	0.0
38	0.0	0.0	0.0	2.997665640920143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCA	30	2.5281133E-6	44.000004	12
CACTCGA	30	2.5281133E-6	44.000004	33
CGCTATT	30	2.5281133E-6	44.000004	42
ATATACG	30	2.5281133E-6	44.000004	1
AACGCCA	30	2.5281133E-6	44.000004	17
AGATTCG	30	2.5281133E-6	44.000004	1
ACTCGAA	30	2.5281133E-6	44.000004	34
TCGTAAT	60	0.0	44.000004	24
CGTAATA	60	0.0	44.000004	25
AATACGC	30	2.5281133E-6	44.000004	11
TACCGCG	30	2.5281133E-6	44.000004	12
AACGTAG	40	8.314601E-9	44.0	1
AGCCGTC	20	7.8570715E-4	44.0	33
ACTATCG	50	2.7284841E-11	44.0	1
ATTAGCG	50	2.7284841E-11	44.0	1
CGAGTTA	25	4.443339E-5	44.0	26
TTTACGT	20	7.8570715E-4	44.0	30
CAACGAA	25	4.443339E-5	44.0	41
CGACGAA	40	8.314601E-9	44.0	43
CTATCGC	20	7.8570715E-4	44.0	44
>>END_MODULE